FastQCFastQC Report
Fri 14 Jul 2023
SRR8763651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821068
Total Bases143.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTACCATCTCGTATG101160.3585875987392009TruSeq Adapter, Index 2 (97% over 36bp)
GTGGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCC93520.3315056567229149No Hit
GGAACGGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGT80230.28439583873908747No Hit
GTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGTG77990.27645558348823923No Hit
CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA75140.2663530265842582No Hit
GCCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGG73260.2596888837844391No Hit
GTCAAATAGGAAGAACTAGAAGATTCCACCTCTGGAAAGCGCCTACACCT58530.20747461599649494No Hit
CCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACC57090.2023701661923782No Hit
CCCGTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGA51600.18290945131418315No Hit
GCCCGATCCAGCAATATCGCGTGAGTGAAGAAGGGCAATGCCGCTTGTAA49320.17482740579099829No Hit
GGGGAATCTTGGACAATGGGCGAAAGCCCGATCCAGCAATATCGCGTGAG48360.17142443925492046No Hit
CGTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGT47240.16745431162949634No Hit
GCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGATGAGCCTGCGTAGT46560.16504387699977457No Hit
GTCGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAG46460.16468940131893312No Hit
GGTGAATCGGGTTGAAAGTGAAAGCCGCCAAAAACTGGCGGAATGCTCTC41030.1454413718492429No Hit
GTGAAATCCGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCT40200.14249922369825896No Hit
CCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGA39110.13863543877708726No Hit
CCACCCTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACAAGTACC37940.1344880733112424No Hit
CCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGTG37880.13427538790273755No Hit
GGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCCAA36650.12991533702838784No Hit
GCTGACATGAGTAACGAAAAATCCTGTGAAAAACACGATCGCCTGCCAGT36630.12984444189221955No Hit
GGGCAATGCCGCTTGTAAAGCTCTTTCGTCGAGTGCGCGATCATGACAGG36370.1289228051220318No Hit
CAGCTCTCTGGGTCCCTACCGACGCTGAGGTGCGAAAGCATGGGGAGCGA35570.1260869996753003No Hit
GCCGGCGATGCGCCCCGGTCGGATGTGGAACGGCGACGAGCCGGTCCGCC34280.1215142633924457No Hit
CCCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGT34120.1209471023030994No Hit
GCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGA33070.11722510765426426No Hit
CGGGTGCGTAACGCGTGGGAATCTGCCGAACAGTTCGGGCCAAATCCTGA32770.11616168061173995No Hit
GTCATGATCGCGCACTCGACGAAAGAGCTTTACAAGCGGCATTGCCCTTC32650.11573630979473022No Hit
ATCGAATTAAACCACATGCTCCACCGCTTGTGCAGGCCCCCGTCAATTCC32590.11552362438622535No Hit
GTGAAAAGAACCCTATTTAGGGAGTGCAATAGAGAACCTGAGATCCGATG32410.11488556816071076No Hit
GTCCGATGCTTCGGGCGAAACCAATTCCCAGGGTGTGACGGGCGGTGTGT31970.11332587516500843No Hit
CTCTGTCTCACTCAAGTGAATTGGTTTCGAGAGCATTCCGCCAGTTTTTG31770.11261692380332554No Hit
CTCCTCTGTCTCACTCAAGTGAATTGGTTTCGAGAGCATTCCGCCAGTTT31590.11197886757781095No Hit
GTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAGGGGGCAGCCCTCGGCC31130.11034827944594032No Hit
CCCTGATACATTTTCGGTGCCATGGAGCTAGACCAGTGAGCTATTACGCT30670.10871769131406969No Hit
ACCGAACCCACGTATGTGGCAAAATACGGGGATGACTTGTGGCTAGGGGT30590.10843411076939655No Hit
CCTCTGTCTCACTCAAGTGAATTGGTTTCGAGAGCATTCCGCCAGTTTTT29990.1063072566843479No Hit
GGCGGTTGACGGCAACGTTAGGAAGTCCGGAGACGTCGGCGGGAGCCTCG29820.10570464802691748No Hit
GCGGTTATGAGTACGACCGGGCGTGGACGGCATTCGGTCCTCCGGATTTT29700.10527927720990775No Hit
GCGGGACACTGCGAGGTGGGTAGTTTATCTGGGGCGGATGCCTCCTAAAG29470.10446398314397243No Hit
AACAAAGTGGCGAACGGGTGCGTAACGCGTGGGAATCTGCCGAACAGTTC29240.10364868907803712No Hit
GGCCGAGTCAGAAAGGCTTTGATGACTCAAGGTTCATATTAGGGAAAGGA29090.10311697555677496No Hit
CCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAG28830.10219533878658721No Hit
CCCTTCTTCACTCACGCGATATTGCTGGATCGGGCTTTCGCCCATTGTCC28300.10031661767812758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)