FastQCFastQC Report
Fri 14 Jul 2023
SRR8763653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13327072
Total Bases666.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAGGCGATCTCGTATG5433764.077234669400751TruSeq Adapter, Index 1 (97% over 36bp)
CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA526660.39518057679886476No Hit
GCCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGG501610.3763842500438206No Hit
GGAACGGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGT501420.3762416830943811No Hit
GTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGTG433310.32513518348216325No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAGGCGAACTCGTATG345570.25929926693575306TruSeq Adapter, Index 1 (97% over 36bp)
GTCGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAG299750.22491812154987983No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAACAGGCGATCTCGTATGCC295370.22163157818911758TruSeq Adapter, Index 1 (97% over 34bp)
GTGGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCC277210.20800517923216744No Hit
GCCGGCGATGCGCCCCGGTCGGATGTGGAACGGCGACGAGCCGGTCCGCC272910.20477866406064288No Hit
CGGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGAT268580.20152963831815424No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAGGCGATCTCGTAT257320.1930806706829527TruSeq Adapter, Index 1 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAACAGGCGATCTCGTATGCCGT238480.17894403211748236TruSeq Adapter, Index 1 (96% over 32bp)
GGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGAT223360.1675987043515635No Hit
CGGCGATGCGCCCCGGTCGGATGTGGAACGGCGACGAGCCGGTCCGCCGA223130.16742612330750523No Hit
GTCAAATAGGAAGAACTAGAAGATTCCACCTCTGGAAAGCGCCTACACCT206170.15470014718912No Hit
CGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCA200530.15046815984786457No Hit
CGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGGCG191270.14351989694360473No Hit
GGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCCAA191090.14338483351781997No Hit
CCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACC173530.13020864598015228No Hit
GGACGCTTTCAGAGGCGAAAGGCCATGGGGAGATACCGTCTGTGATCCAT171770.12888802581692363No Hit
GGCGGCCAAAGCCCGGGCTCTCGATACGCCCGTGGAACGCCGTCTCCCCG170140.12766495146120618No Hit
CCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGTG165430.12413079181983859No Hit
CCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGGC161050.12084424845907639No Hit
GGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGATT158770.1191334450658029No Hit
GTGAAATCCGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCT158420.11887082173788811No Hit
GGTTGATTTCCCTTCCTTTAGCTACTAAGATGTTTCAGTTCGCTAAGTTT157770.11838309270033208No Hit
GGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCG156490.11742264167252943No Hit
CTTCCTTTAGCTACTAAGATGTTTCAGTTCGCTAAGTTTGAAAAGTCCAA149960.11252284072600494No Hit
CGTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGT148590.1114948579853099No Hit
GCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGATGAGCCTGCGTAGT147610.1107595126671485No Hit
GTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCGAAAA146930.11024927305862833No Hit
GCCCGATCCAGCAATATCGCGTGAGTGAAGAAGGGCAATGCCGCTTGTAA146570.10997914620705883No Hit
CCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAG145080.1088611211825073No Hit
GGTGAATCGGGTTGAAAGTGAAAGCCGCCAAAAACTGGCGGAATGCTCTC144210.10820831462454769No Hit
GACGCTTTCAGAGGCGAAAGGCCATGGGGAGATACCGTCTGTGATCCATG141710.10633243371087062No Hit
CTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAAGCTCCGTGGCT141460.10614484561950292No Hit
GCCGCTACTAACGGGGTCTCGGTTGATTTCCCTTCCTTTAGCTACTAAGA139660.10479421136165544No Hit
CGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCTGGGTCCCT139000.10429897880044468No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAGGCGAGCTCGTATG138110.10363116519517565TruSeq Adapter, Index 1 (97% over 36bp)
GCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGA137790.103391052438225No Hit
CCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGA136580.10248312607600531No Hit
GGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCGGA136070.10210044636961516No Hit
CAGCTCTCTGGGTCCCTACCGACGCTGAGGTGCGAAAGCATGGGGAGCGA134460.10089237906120714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)