FastQCFastQC Report
Fri 14 Jul 2023
SRR8763782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3698963
Total Bases188.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGAT588541.5910945851580567No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA478281.293011041202629Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG368630.9965766080925925No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG347650.9398580088527514No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAA321410.8689192078969159No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT280940.7595101654166316No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA271730.7346112951116299No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA268480.7258250488042189No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATCG265790.7185527403220848No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG228300.6172000098405959No Hit
ATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATC196930.5323924570210624No Hit
TCCCCGGCAGCGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG195020.527228847652707No Hit
AACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT176830.47805290293522806No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG169170.4573443962537609No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC157190.42495694063444267No Hit
TGCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGA149140.40319408439608617No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT141830.38343178885541707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC140570.38002542874854384No Hit
ATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGAA136980.37032000590435754No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA136290.3684546182267841No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAAT127120.34366388633787365No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAATA118680.3208466805426277No Hit
TTGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGA115510.3122767110673991No Hit
TGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCA108650.2937309727077562No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC98400.26602050358438295No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT96510.26091096342407316No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG95860.259153714162591No Hit
AGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGAAGAG95430.25799122618961046No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA90230.2439332320977528No Hit
AACCTGCATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC89750.24263557110465825No Hit
TTGCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAG86730.23447112068977172No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAATA83840.2266581201271816No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAA80660.2180611160479302No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG79420.2147088251491026No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATAG79030.2136544755922133No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCCTGTAGGCACCATCAATAGA76320.20632809790203363No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGCTGTAGGCACCATCAATAG75040.2028676685871148No Hit
TGAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAG74150.20046158882908535No Hit
TTTCGCTTGCCGACCAGCAGTAGGGGGCCCTGTAGGCACCATCAATAGAT73190.19786626684289624No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAAT73190.19786626684289624No Hit
TTGATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCA69530.18797160177055028No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG68970.1864576639452733No Hit
ATCCTGCCGTCCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG68300.1846463454757455No Hit
ATGTACACGGATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA67100.1814021929930091No Hit
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA65420.17686037951717817No Hit
GCTGGAATAGCTCAGTTGGTTAGAGCTTGTGGCTGTAGGCACCATCAATA64240.17367029624248742No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA63940.17285925812180333No Hit
GCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT63400.17139938950457195No Hit
TTGCCCCTCTTTTTAGATCCAAGGCCGACTCTGTAGGCACCATCAATAGA62870.16996655549136339No Hit
TGGGCATTTGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAG61190.16542474201553245No Hit
TGGAATCGGGTTAAAATTCCTGAACCGGGCTGTAGGCACCATCAATAGAT61010.164938119143122No Hit
CCGACCTTAGCTCAGTTGGTAGAGCTGAGGACTGTAGGCACCATCAATAG60640.16393783879427828No Hit
TCGGAGACGTCATCATCACGATTGTGGGCTCTGTAGGCACCATCAATAGA60270.1629375584454346No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA59310.16034223645924547No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT56010.15142081713172043No Hit
TATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGA55780.15079902123919595No Hit
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATCA55250.1493661872259874No Hit
GCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCGGAAGAG53960.1458787233070458No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA53420.1444188546898144No Hit
CCAGGAGACCCGGGTTCGTTTCCCGGCAATGGACTGTAGGCACCATCAAT51970.14049883710650796No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT50520.1365788195232015No Hit
GCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAGATCGGAAGA50170.1356326083824034No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG49330.13336170164448793No Hit
GTCTAGCGGTTAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA46090.12460248994109971No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC45040.12176385651870537No Hit
GAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGA44870.12130426825031772No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAA44270.11968219200894953No Hit
GTGTAGTGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG43390.11730314685494286No Hit
GTGTAGTGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA41090.11108518792969813No Hit
ACGTCATCATCACGATTGTGGGCTACAGCGCGCCTGTAGGCACCATCAAT40110.10843579673546343No Hit
CCAGGAGACCCGGGTTCGTTTCCCGGCAATGGCTGTAGGCACCATCAATA39990.1081113814871898No Hit
TGTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAA39530.10686778970214084No Hit
TTCTCGGAATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC38920.10521867885674985No Hit
TTCTCGGAATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT38590.10432653692399735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAAT38520.10413729469583773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)