FastQCFastQC Report
Fri 14 Jul 2023
SRR8763784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33420664
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATCG11140483.3334107305587946No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG8367082.503564860351069No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG5049501.5108915849188393No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4523671.3535547947222113Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA3887861.163310220287664No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC3425341.0249167999774034No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT3081360.9219924535311447No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG2535890.7587790595662611No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAATA2307500.6904410995544553No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATAG2235070.6687688790384296No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCTGTAGGCACCATCAATAGATCGG1782330.533301791969184No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1656980.49579505661527257No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1616460.48367082114227294No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA1613830.4828838828576236No Hit
GCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCGGAAGAG1552850.4646376864325616No Hit
TAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAGATCGGAA1498620.4484111985327401No Hit
AACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1493680.44693307110834185No Hit
ATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGATCGGAAG1483960.4440246908319954No Hit
ACGATTGTGGGCTACAGCGCGCGCTGTAGGCACCATCAATAGATCGGAAG1390400.4160300345917723No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAATA1380620.41310370135075714No Hit
ATCCTGCCGTCCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1251590.37449585083049214No Hit
GCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1228540.36759892023689295No Hit
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATCA1182110.3537063177440161No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1156110.3459266997208673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA1086900.32521795497540085No Hit
ACGATTGTGGGCTACAGCGCGCCTGTAGGCACCATCAATAGATCGGAAGA1061940.317749521673178No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA972070.29085897276008643No Hit
AGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGAT968480.28978478704073624No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT929980.2782649680449198No Hit
TATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATAGA922070.27589816886941565No Hit
TGGAATCGGGTTAAAATTCCTGAACCGGGCTGTAGGCACCATCAATAGAT904780.2707247228840217No Hit
GTCTAGCGGTTAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA873960.26150288336581223No Hit
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCA856060.2561469155729521No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGACGTCTGTAGGCACCATCAAT841100.25167064304886344No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGCTGTAGGCACCATCAATAG825750.2470776762544275No Hit
CCGACCTTAGCTCAGTTGGTAGAGCTGAGGACTGTAGGCACCATCAATAG820770.2455875801869167No Hit
TACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATC786340.23528557062780078No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA778110.2328230223073964No Hit
TGCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGA775460.23203009970119087No Hit
TGAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAG757130.226545468994871No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGAT739680.22132414843702686No Hit
ATAGCTCAGTGGTAGAGCATTTGACTGTAGGCACCATCAATAGATCGGAA709730.21236262690651508No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAGATC700990.20974747838642585No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT700530.20960983899063168No Hit
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCATC698030.20886179879609812No Hit
GAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGA659290.1972701679416064No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC615230.18408670755314735No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC614070.18373961690288382No Hit
AACCTGCATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC613440.18355111077386133No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA612670.183320714393945No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA591590.17701323947363823No Hit
GCTGGAATAGCTCAGTTGGTTAGAGCTTGTGGCTGTAGGCACCATCAATA584980.17503542119929155No Hit
TCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAGC579770.17347650543388368No Hit
AGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAGATCGGAAG579710.17345855246921485No Hit
TGGGCATTTGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAG576350.17245318644776178No Hit
ATCTCTCAGGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG552580.1653408202781369No Hit
ATCACGATTGTGGGCTACAGCGCGCGCTGTAGGCACCATCAATAGATCGG545240.16314457426698645No Hit
GTCTAGTGGTAGAATAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC527770.15791726938758607No Hit
ACCCTGCTGTTGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG510970.1528904392803207No Hit
ATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATAGAT503980.15079891889640493No Hit
TGTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGG501930.15018552593688744No Hit
AGAGTGGCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCG491190.14697194526117136No Hit
TAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAAT470570.14080210973665874No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG470250.14070636059175845No Hit
ATAGCTCAGTTGGTTAGAGCACCCGTCTGTAGGCACCATCAATAGATCGG468750.14025753647503833No Hit
GTGTAGTGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG464560.13900382110900011No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATC456410.13656521007482078No Hit
ATCACGATTGTGGGCTACAGCGCGCCTGTAGGCACCATCAATAGATCGGA451480.13509007481120064No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG443660.13275020508269972No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT443440.13268437754558077No Hit
ACGGGGGTCCCAGTTTCGAACCCGTCGGCCTGTAGGCACCATCAATAGAT434280.1299435582728099No Hit
TTCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA425170.1272176998039297No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA421110.12600288252800723No Hit
TCCCCAGTGAAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT421060.12598792172411655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCTTGCCGACCAGCAGTAGGGGGCCCTGTAGGCACCATCAATAGAT420370.1257814626304253No Hit
GGAATCGGGTTAAAATTCCTGAACCGGGCTGTAGGCACCATCAATAGATC420350.12577547830886904No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCCTGTAGGCACCATCAATAGATCG406010.12148471975302465No Hit
TTGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATC403340.12068581282526285No Hit
ATTTGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGG400980.11997966288162319No Hit
GCCCAATGGATAAGGCTCTGGTCTGTAGGCACCATCAATAGATCGGAAGA399920.11966249383914096No Hit
TTGCCCCTCTTTTTAGATCCAAGGCCCTGTAGGCACCATCAATAGATCGG397980.11908201464818292No Hit
ACCCTGCTGTCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC386700.1157068572904476No Hit
TGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAG383300.114689522625882No Hit
TGTGTCTCGGTCGGATGTGGAACGGCTGTAGGCACCATCAATAGATCGGA382670.11450101649685955No Hit
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT379570.11357344665563797No Hit
ACGTGCCGTTGGTGGACCTCGTAAGGCCTGTAGGCACCATCAATAGATCG372030.11131735742892482No Hit
TAGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAGATCGGAA365520.10936946076235948No Hit
TCCCCGGCAGCGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG362090.10834314961545946No Hit
ACTGACCAGGGTTCGTTTCCCTGGATGCGCCTGTAGGCACCATCAATAGA344610.10311285257528097No Hit
ATAGCTCAGTGGTAGAGCATTTGCTGTAGGCACCATCAATAGATCGGAAG341270.10211347087538415No Hit
CGCTTGCCGACCAGCAGTAGGGGGCCCTGTAGGCACCATCAATAGATCGG337670.10103629299525586No Hit
TTGACCGACGCGGATTGTGATGGTGGCTGTAGGCACCATCAATAGATCGG337500.1009854262620276No Hit
GTCTAGTGGTAGAATAGTACTGTAGGCACCATCAATAGATCGGAAGAGCA336290.10062337480787335No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCAAT336090.10056353159231066No Hit
ACCTCGGCTTACCTCTGGTTCAGAGGACTGTAGGCACCATCAATAGATCG335850.10049171973363547No Hit
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA334660.10013565260103749No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)