FastQCFastQC Report
Mon 17 Jul 2023
SRR8763785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17346037
Total Bases884.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATCG6259143.6083976991401556No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG4149242.3920391729822783No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC2509731.4468607440419965No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG2422481.396561070404727No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT2169771.2508736145322414No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA1936021.1161166092289554No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1891171.0902605592274477Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAATA1422060.8198183827233853No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATAG1369570.7895578684629809No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1317030.7592685291747043No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA1039040.5990071392099533No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCTGTAGGCACCATCAATAGATCGG980080.5650166663428655No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAATA883200.5091652923373794No Hit
TAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAGATCGGAA801960.4623303870503677No Hit
GCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCGGAAGAG795020.45832947318168404No Hit
ATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGATCGGAAG783920.45193031699402003No Hit
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATCA779500.44938218453010337No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG735530.4240334550191493No Hit
ACGATTGTGGGCTACAGCGCGCGCTGTAGGCACCATCAATAGATCGGAAG729230.4204015015072319No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC672090.38746025965469805No Hit
AACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT654850.3775213900443081No Hit
GCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAGATCGGAAGA636640.36702331489319434No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA623120.359229027356508No Hit
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCA621880.35851416666527347No Hit
TATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATAGA572260.3299082090047427No Hit
AGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGAT561590.3237569480567809No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT558580.32202168137886483No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA554030.31939860384248No Hit
ATCCTGCCGTCCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG549420.3167409362726483No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGACGTCTGTAGGCACCATCAAT543940.31358171321783757No Hit
ACGATTGTGGGCTACAGCGCGCCTGTAGGCACCATCAATAGATCGGAAGA541770.31233070700817717No Hit
TGGAATCGGGTTAAAATTCCTGAACCGGGCTGTAGGCACCATCAATAGAT538570.31048590522434605No Hit
CCGACCTTAGCTCAGTTGGTAGAGCTGAGGACTGTAGGCACCATCAATAG519510.2994978045994022No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGCTGTAGGCACCATCAATAG515950.2974454626148901No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA501270.28898243443156496No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT474860.27375705470938405Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCATC474360.2734688044306604No Hit
TACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATC465440.26832641945823127No Hit
TGCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGA462580.26667762786393223No Hit
TGAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAG461690.26616454236780424No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGAT445080.256588868108606No Hit
GTCTAGCGGTTAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA437370.25214404881068797No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAGATC404230.2330388203368873No Hit
GAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGA402010.23175898909935452No Hit
GCTGGAATAGCTCAGTTGGTTAGAGCTTGTGGCTGTAGGCACCATCAATA372760.2148963477940235No Hit
ATAGCTCAGTGGTAGAGCATTTGACTGTAGGCACCATCAATAGATCGGAA361170.2082147063332103No Hit
TGGGCATTTGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAG359480.2072404203911245No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC337630.19464388321090287No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA309840.1786229327194448No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATC308610.17791383703378472No Hit
ATCACGATTGTGGGCTACAGCGCGCGCTGTAGGCACCATCAATAGATCGG304800.17571736990991083No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA302880.1746104888396122No Hit
AGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAGATCGGAAG302050.174131993376931No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT301760.1739648082152713No Hit
ATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATAGAT301310.17370538296442004No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG297150.17130714064543964No Hit
TAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAAT294100.16954881394522564No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC293260.16906455347696997No Hit
TCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAGC286560.16520199974207364No Hit
TGTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGG286470.1651501146919034No Hit
AGAGTGGCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCG286070.16491951446892453No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT281240.16213501677645448No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG280580.1617545264085393No Hit
TTCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA278550.16058423027692148No Hit
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT276510.15940816913972913No Hit
TTGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATC270300.15582810067798195No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA266980.1539141188272572No Hit
ACGGGGGTCCCAGTTTCGAACCCGTCGGCCTGTAGGCACCATCAATAGAT263090.15167153165878752No Hit
AACCTGCATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC262390.1512679812685745No Hit
ATAGCTCAGTTGGTTAGAGCACCCGTCTGTAGGCACCATCAATAGATCGG262340.1512391562407021No Hit
TTTCGCTTGCCGACCAGCAGTAGGGGGCCCTGTAGGCACCATCAATAGAT251210.14482270503631464No Hit
GTCTAGTGGTAGAATAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC247110.14245905275078105No Hit
ATCTCTCAGGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG243590.14042977078856686No Hit
ATCACGATTGTGGGCTACAGCGCGCCTGTAGGCACCATCAATAGATCGGA243280.14025105561575824No Hit
GGAATCGGGTTAAAATTCCTGAACCGGGCTGTAGGCACCATCAATAGATC241540.1392479446458001No Hit
GTGTAGTGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG239160.13787587331907572No Hit
ATTTGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGG227400.13109622676349647No Hit
TTGCCCCTCTTTTTAGATCCAAGGCCCTGTAGGCACCATCAATAGATCGG223250.12870374945009053No Hit
ACCCTGCTGTTGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG223090.12861150936089896No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCCTGTAGGCACCATCAATAGATCG222200.12809842386477097No Hit
TAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCAC222020.12799465376443048No Hit
TGTGTCTCGGTCGGATGTGGAACGGCTGTAGGCACCATCAATAGATCGGA215560.12427046016332144No Hit
ACGTGCCGTTGGTGGACCTCGTAAGGCCTGTAGGCACCATCAATAGATCG214310.12354983446651244No Hit
ACTGACCAGGGTTCGTTTCCCTGGATGCGCCTGTAGGCACCATCAATAGA211800.12210281806731993No Hit
CGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCATC211660.12202210798927732No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCAAT205920.11871299478953032No Hit
ACGTGGTCGTGGGTTCGTTTCCCACAGACGGCCTGTAGGCACCATCAATA203470.11730056842378464No Hit
GCCCAATGGATAAGGCTCTGGTCTGTAGGCACCATCAATAGATCGGAAGA201740.11630322245940097No Hit
CCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT201580.11621098237020941No Hit
TAGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAGATCGGAA196170.11309211435441997No Hit
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA194880.11234842863531308No Hit
TGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAG194860.11233689862416413No Hit
CGCTTGCCGACCAGCAGTAGGGGGCCCTGTAGGCACCATCAATAGATCGG194560.11216394845692997No Hit
ACCTCGGCTTACCTCTGGTTCAGAGGACTGTAGGCACCATCAATAGATCG192910.11121272253714207No Hit
CCGACCTTAGCTCAGTTGGTAGAGCGGAGGACTGTAGGCACCATCAATAG192170.1107861121246311No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGCTGTAGGCACCATCAATAGAT190820.11000783637207738No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT189700.1093621557477365No Hit
TTGACCGACGCGGATTGTGATGGTGGCTGTAGGCACCATCAATAGATCGG185050.10668142815560697No Hit
TGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCGG182320.10510758163377606No Hit
CCGACCTTAGCTCAGTTGGTAGAGCGAGGACTGTAGGCACCATCAATAGA182170.10502110655015898No Hit
TCCCCAGTGAAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT181840.1048308613662014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGGA180370.10398340554675399No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGACGCTGTAGGCACCATCAATA179550.10351067508964729No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGACTGTAGGCACCATCAATAG175840.1013718580215181No Hit
ATAGCTCAGTGGTAGAGCATTTGCTGTAGGCACCATCAATAGATCGGAAG174790.10076653243619854No Hit
TTGCCCCTCTTTTTAGATCCAAGGCCGCTGTAGGCACCATCAATAGATCG174660.10069158736373039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)