FastQCFastQC Report
Fri 14 Jul 2023
SRR8763786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33358480
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATCG7766512.3281966084785637No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC3988251.1955730596837746No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG3817401.1443566973075512No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT3625371.0867911247754694No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT3248360.9737733853580859No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA2918280.8748240327496937No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG2798570.8389381050935174No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA2063970.6187242344375403No Hit
TGCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGA1950730.584777843594792No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA1930230.5786324796573465No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAATA1842920.5524592247608404No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA1808200.5420510766677618No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGAT1781560.5340651012875887No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATAG1774130.5318377815775779No Hit
AGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGAAGAG1465080.43919267304745296No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1453840.4358232149666292Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAA1364250.4089664756907389No Hit
ATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGAA1269600.38059288073077674No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAAT1209190.36248354241560166No Hit
TCCCCGGCAGCGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1168250.35021080097174695No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1115060.33426582985795517No Hit
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA1099380.3295653758804358No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAATA1073560.3218252150577604No Hit
AACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1057390.31697787189344356No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAAT1034140.31000812986682846No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG1018460.3053076758893091No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAGA1015940.3045522457857792No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGACGTCTGTAGGCACCATCAAT984510.295130353661198No Hit
ATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATC982040.29438991225019845No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC977860.29313685755466073No Hit
GCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAGATCGGAAGA976180.29263323748564085No Hit
TGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCA947330.28398476189562594No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCTGTAGGCACCATCAATAGATCGG904830.2712443732448241No Hit
GCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCGGAAGAG861200.25816524014283626No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAGATC818330.2453139351673098No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT811830.2433654051383636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATCA766670.2298276180449469No Hit
TTGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGA759700.22773819430621536No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC727210.21799854189999066No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG703680.21094486319520553No Hit
AGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGAT696300.2087325321777251No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA696040.2086545909765673No Hit
TGAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAG680380.20396013247606007No Hit
TTGATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCA653880.19601612543497185No Hit
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCA652600.1956324149061948No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG639780.1917893141414117No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGCTGTAGGCACCATCAATAG600320.17996023799645547No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAA596800.17890503404231847No Hit
CCGACCTTAGCTCAGTTGGTAGAGCTGAGGACTGTAGGCACCATCAATAG570200.17093104961616956No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG569580.17074518982879316No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCTGTAGGCACCATCAATAGATC560440.16800525683424425No Hit
ATCACGATTGTGGGCTACAGCGCGCGCTGTAGGCACCATCAATAGATCGG558640.16746566390315146No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA541600.16235751748880645No Hit
ATCCTGCCGTCCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG538810.16152114844561263No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCCTGTAGGCACCATCAATAGATCG538410.16140123890536978No Hit
GCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT537320.16107448540820807No Hit
ATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATAGAT527310.15807374916363096No Hit
TGCACCGGTTGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAG522620.15666780980428363No Hit
ACGGGGGTCCCAGTTTCGAACCCGTCGGCCTGTAGGCACCATCAATAGAT521390.1562990879680369No Hit
TATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGA518300.15537278676966096No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCTGTAGGCACCATCAATA506530.15184444854801538No Hit
TACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATC501310.1502796290478463No Hit
GAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGA492900.14775853096424058No Hit
TAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAAT489600.14676927725723712No Hit
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCATC478260.1433698417913526No Hit
ATCAGAGTGGCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGA472460.14163115345783142No Hit
TAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCAC469100.14062391331979154No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAA462620.13868137876785752No Hit
TTGACCGACGCGGATTGTGATGGTGGCTGTAGGCACCATCAATAGATCGG458070.1373174077475952No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATC454700.1363071698710493No Hit
TTCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA453410.1359204616037661No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGCTGTAGGCACCATCAATAGAT452010.13550077821291617No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT441140.13224223645681696No Hit
TTTCGCTTGCCGACCAGCAGTAGGGGGCCCTGTAGGCACCATCAATAGAT430300.128992687916236No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA422480.12664845640448846No Hit
TGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCGG419110.12563821852794252No Hit
AACCTGCATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC415140.12444811634103232No Hit
GTCTAGCGGTTAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA414630.1242952316772227No Hit
GCTGGAATAGCTCAGTTGGTTAGAGCTTGTGGCTGTAGGCACCATCAATA411070.12322803676906141No Hit
GTGTAGTGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG405910.12168120369992878No Hit
TGGGCATTTGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAG404630.12129749317115168No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATC395410.11853357826855421No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT395290.11849760540648135No Hit
TGTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGG392660.11770920017938467No Hit
ACGATTGTGGGCTACAGCGCGCGCTGTAGGCACCATCAATAGATCGGAAG389140.11665399622524768No Hit
TCCATTGTCGTCCAGCGGTTAGGATGTCTGGCCTGTAGGCACCATCAATA386140.11575467467342636No Hit
TTGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCG383440.1149452852767872No Hit
ATCACGATTGTGGGCTACAGCGCGCCTGTAGGCACCATCAATAGATCGGA381470.11435473079109121No Hit
TCAGCGGCGGACCGGGCCCAAGTCCCCTCTGTAGGCACCATCAATAGATC379330.113713214750792No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCCTGTAGGCACCATCAATAGA374170.11216638168165935No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC361990.10851513618126485No Hit
TTCGCGCTTGGTTGAAAAGCCAGTGGCGCGACTGTAGGCACCATCAATAG361140.10826032840824881No Hit
TGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAG359580.10779268120130175No Hit
GTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT359400.10773872190819246No Hit
ATCAGAGTGGCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAG355650.10661456996841581No Hit
TATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATAGA353820.10606598382180484No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCAAT350710.10513368714641674No Hit
TCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAGC345250.10349692192210197No Hit
TCGGAGACGTCATCATCACGATTGTGGGCTCTGTAGGCACCATCAATAGA338990.1016203376173015No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT336320.10081994143618055No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)