FastQCFastQC Report
Fri 14 Jul 2023
SRR8763787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13360061
Total Bases681.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATCG3158792.364352977130868No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC2112371.5811080503300097No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT1851341.3857272058862606No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT1418061.0614173093970156No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA1356911.015646560296394No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG1346711.0080118646164864No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA976220.7307002565332599No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG969920.7259847092015522No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA889230.6655882783768727No Hit
TGCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGA860810.6443159204138365No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAATA821630.6149897070080743No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATAG783560.5864943281321844No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGAT769520.5759853940786648No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAA654780.49010255267547054No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA654270.4897208178914752No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAAT585630.4383438069631569No Hit
AGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGAAGAG530410.39701166035095203No Hit
TGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCA525380.39324670748135054No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA521950.39067935393408754Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA516980.3869593110390738No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAAT506790.37933210035493103No Hit
ATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGAA497890.3726704541244235No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAATA488640.36574683304215455No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAGA461730.34560470943957516No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGACGTCTGTAGGCACCATCAAT460210.3444669900833537No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG457830.34268556109137527No Hit
TCCCCGGCAGCGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG439350.32885328891836646No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG414220.3100434945618886No Hit
ATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATC412590.30882344025225633No Hit
AACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT396700.29692978198228287No Hit
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATCA368130.2755451490827774No Hit
GCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAGATCGGAAGA362030.2709793016663622No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCTGTAGGCACCATCAATAGATCGG360400.26975924735672985No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC352700.26399580061797623No Hit
ACCGGTTGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAGATC341420.25555272539549034No Hit
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCA340860.25513356563267187No Hit
TTGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGA334930.2506949631442551No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG322290.24123392849778155No Hit
TTGATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCA319520.23916058467098317No Hit
GCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCGGAAGAG317940.23797795534017396No Hit
TGAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAG298120.22314269373470674No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG291210.21797056166135767No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAA288580.21600200777526388No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT287690.21533584315221316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGAT286310.21430291373669627No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC286270.21427297375363782No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGCTGTAGGCACCATCAATAG270130.20219219058954896No Hit
CCGACCTTAGCTCAGTTGGTAGAGCTGAGGACTGTAGGCACCATCAATAG260000.1946098898799938No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA259940.1945649799054061No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA255380.19115182183674162No Hit
TAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCAC253490.18973715763722934No Hit
TCACCGGTTGGCTCGTCCCTTCTGCCGGCTGTAGGCACCATCAATAGATC246780.1847147254791726No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCTGTAGGCACCATCAATA239400.17919079860488663No Hit
TGCACCGGTTGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAG238110.17822523415125124No Hit
GCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT236730.17719230473573436No Hit
TGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCATC236160.17676565997715132No Hit
ATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATAGAT229730.17195280770050378No Hit
ACGGGGGTCCCAGTTTCGAACCCGTCGGCCTGTAGGCACCATCAATAGAT229420.1717207728318007No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAA228770.17123424810710072No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATC223740.1674692952374993No Hit
ATCACGATTGTGGGCTACAGCGCGCGCTGTAGGCACCATCAATAGATCGG221420.16573277622010857No Hit
TAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAAT220370.16494685166482398No Hit
GAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGA217040.16245434807520714No Hit
TTCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA216260.16187051840556715No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCCTGTAGGCACCATCAATAGATCG215930.16162351354533486No Hit
TATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGA211570.15836005539196266No Hit
TACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGATC211240.15811305053173036No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG207390.15523132716235352No Hit
ATCAGAGTGGCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGA206520.154580132530832No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT200090.14976728025418445No Hit
ATCCTGCCGTCCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG199860.14959512535159833No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA193960.1451789778504754No Hit
GCTGGAATAGCTCAGTTGGTTAGAGCTTGTGGCTGTAGGCACCATCAATA193350.14472239310883386No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGCTGTAGGCACCATCAATAGAT188330.14096492523499707No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT186130.13931822616678172No Hit
TGGGCATTTGGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAG184870.13837511670044023No Hit
TTTCGCTTGCCGACCAGCAGTAGGGGGCCCTGTAGGCACCATCAATAGAT184440.13805326188256176No Hit
TTGACCGACGCGGATTGTGATGGTGGCTGTAGGCACCATCAATAGATCGG182630.13669847764916643No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC181950.13618949793717258No Hit
TCCATTGTCGTCCAGCGGTTAGGATGTCTGGCCTGTAGGCACCATCAATA179940.13468501378848496No Hit
TGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCGG170490.12761169279092363No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCCTGTAGGCACCATCAATAGA166270.12445302457825605No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCAAT163110.12208776591663767No Hit
GTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT162970.12198297597593305No Hit
TCAGCGGCGGACCGGGCCCAAGTCCCCTCTGTAGGCACCATCAATAGATC162880.1219156110140515No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATC162320.12149645125123305No Hit
TGTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGG162260.12145154127664536No Hit
TTCGCGCTTGGTTGAAAAGCCAGTGGCGCGACTGTAGGCACCATCAATAG161340.12076292166630079No Hit
TATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATAGA158040.11829287306397779No Hit
ATCAGAGTGGCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAG152900.11444558524096558No Hit
TTGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCG152830.11439319027061329No Hit
TCGGAGACGTCATCATCACGATTGTGGGCTCTGTAGGCACCATCAATAGA152670.11427343033837944No Hit
ACGTCATCATCACGATTGTGGGCTACAGCGCGCCTGTAGGCACCATCAAT151210.11318062095674562No Hit
GTGTAGTGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG151210.11318062095674562No Hit
AACCTGCATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC151050.11306086102451178No Hit
ATGTACACGGATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA150120.11236475641840259No Hit
GTCTAGCGGTTAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA148590.11121955206641647No Hit
TGTGCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATA148290.110995002193478No Hit
ATCACGATTGTGGGCTACAGCGCGCCTGTAGGCACCATCAATAGATCGGA148040.11080787729936263No Hit
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT147600.11047853748571956No Hit
ACGATTGTGGGCTACAGCGCGCGCTGTAGGCACCATCAATAGATCGGAAG144510.10816567379445348No Hit
TGTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAA142950.10699801445517353No Hit
TCGGCGACGTGGGCGGTTCGCTGCCCGCGACGCTGTAGGCACCATCAATA142330.10653394471776738No Hit
TGGAATCGGGTTAAAATTCCTGAACCGGGCTGTAGGCACCATCAATAGAT139980.10477497071308282No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGACTGTAGGCACCATCAATAG139240.10422108102650131No Hit
CCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAGAT139140.10414623106885515No Hit
TTGCACCGGTTGGCTCGTCCCTTCTGCCGGCCTGTAGGCACCATCAATAG136640.10227498212770138No Hit
TTCGCTTGCCGACCAGCAGTAGGGGGCCCTGTAGGCACCATCAATAGATC136280.10200552228017523No Hit
TTGCCCCTCTTTTTAGATCCAAGGCCGCTGTAGGCACCATCAATAGATCG135250.10123456771641987No Hit
TTGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCCTGTAGGCACCATC133940.10025403327125527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)