FastQCFastQC Report
Fri 14 Jul 2023
SRR8763788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10649591
Total Bases543.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3602913.3831440099436687Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC2895752.7191185088704346No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG1309701.2298124876345016No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAATA1304531.2249578411039448No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA996980.9361674077436402No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG964940.9060817452989509No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATAG877860.8243133468693774No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG799350.7505922058415202No Hit
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA789040.7409110828763282No Hit
ATGTACACGGATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA661120.6207937938649475No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA643550.6042955076866332No Hit
ATGAGAATCGGTCGCCCTCGGCGTTCGACTGTAGGCACCATCAATAGATC554070.5202735015833002No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATC536770.5040287462682839No Hit
GCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAGATCGGAAGA521530.4897183375399111No Hit
TGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCGG439270.41247593452180464No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA430870.40458830766364645No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAAT425510.39955525052558355No Hit
GTCTAGTGGTAGAATAGTACCCTCTGTAGGCACCATCAATAGATCGGAAG418030.39253150660903313No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC400480.37605200049466686No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA393340.36934751766523244No Hit
GAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGA387210.3635914280651717No Hit
TGTGCCGTTGGTGGACCTCGTAAGGCGCTGTAGGCACCATCAATAGATCG377810.3547647980096137No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC370380.34778800425293327No Hit
TAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAAT338270.31763661158442613No Hit
TGTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGG327420.3074484268926384No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA317500.29813351517443254No Hit
TCAGCGGCGGACCGGGCCCAAGTCCCCTCTGTAGGCACCATCAATAGATC311680.29266851656556575No Hit
ATGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCG307410.2886589729126687No Hit
CCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA301190.28281837302484203No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA294260.27631108086686146No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCCTGTAGGCACCATCAATAGATCG282450.26522145310557No Hit
AGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGAT279240.26220725284191665No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAA278520.2615311705397888No Hit
TGCTTGAGCTGCTCCCGCGGCGAGAGCGCTGTAGGCACCATCAATAGATC273490.25680798445686787No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCG256330.24069468958948753No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAAT256210.24058200920579953No Hit
GTCTAGTGGTAGAATAGTACCCTGCTGTAGGCACCATCAATAGATCGGAA238900.2243278638588092No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA231840.21769850128516674No Hit
ATGTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCG226630.21280629462671385No Hit
ATGAGAATCGGTCGCCCTCGGCGTTCGAACTGTAGGCACCATCAATAGAT223450.2098202644589825No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCACTGTAGGCAC222970.20936954292423063No Hit
TGAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAG212690.19971659005496079No Hit
GTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT208580.19585728691364768No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAA208060.1953690052509998No Hit
TGAGAATCGGTCGCCCTCGGCGTTCGACTGTAGGCACCATCAATAGATCG199710.18752832855271157No Hit
CGTGCCGTTGGTGGACCTCGTAAGGCGCTGTAGGCACCATCAATAGATCG197390.18534984113474406No Hit
TGCTCGCGGAGACGTCATCATCACGATTCTGTAGGCACCATCAATAGATC196510.18452351832103223No Hit
TGGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAA193580.18177223895265085No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG189840.17826036699437564No Hit
ATGAGAATCGGTCGCCCTCGGCGTTCGAATTCTGTAGGCACCATCAATAG189540.17797866603515572No Hit
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTCTGTAGGCACCATCAA184670.17340572046381875No Hit
TTGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCG179590.16863558422102784No Hit
CTCAGCGGCGGACCGGGCCCAAGTCCCCCTGTAGGCACCATCAATAGATC178500.16761207073586207No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAAT172060.16156489014460743No Hit
TCAGCGGCGGACCGGGCCCAAGTCCCCCTGTAGGCACCATCAATAGATCG168200.15794033780264424No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC167170.15697316450932247No Hit
TGTGTCTCGGTCGGATGTGGAACGGCTGTAGGCACCATCAATAGATCGGA165870.15575246035270274No Hit
CTCAGCGGCGGACCGGGCCCAAGTCCCCTCTGTAGGCACCATCAATAGAT163310.1533486121673593No Hit
TTGAGAATCGGTCGCCCTCGGCGTTCGACTGTAGGCACCATCAATAGATC159510.1497804000172401No Hit
TAGTAAGCGCGAGTCATCAGCTCGCGTTGACTCTGTAGGCACCATCAATA157650.1480338540700765No Hit
AGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGAAGAG156690.1471324110005727No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT154790.1453483049255131No Hit
GTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGGA152340.14304774709188361No Hit
GTGTAGTGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG148560.13949831500571244No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGAT147930.13890674299135056No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT143590.13483146911463548Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTAGCGGTTAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA142790.13408026655671565No Hit
TTGACCGACGCGGATTGTGATGGTGGCTGTAGGCACCATCAATAGATCGG141870.13321638361510785No Hit
TGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCA141380.13275627204838195No Hit
TGTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAA140650.1320707997142801No Hit
TGGTGTGCACCGGTTGGCTCGTCCCTTCTGTAGGCACCATCAATAGATCG136870.12852136762810892No Hit
ATGGTGTGCACCGGTTGGCTCGTCCCCTGTAGGCACCATCAATAGATCGG136000.1277044348463711No Hit
GTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA133010.12489681528614573No Hit
TGGAGCCCGAGGGCGAGTTCTATCGGGCTGTAGGCACCATCAATAGATCG130040.12210797578986836No Hit
TTGACCGACGCGGATTGTGATGGTGGCCCTGTAGGCACCATCAATAGATC129630.12172298447893444No Hit
GGGTCCATAGCTCAGTGGTAGAGCATTTGACTCTGTAGGCACCATCAATA123930.11637066625375565No Hit
AGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAATA123450.11591994471900377No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCTTCTGTAGGCACCATCAATAGAT121300.11390108784459423No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA121190.11379779749288024No Hit
GTCTAGCGGTTAGGACATTGGACTCTGTAGGCACCATCAATAGATCGGAA120870.11349731646971231No Hit
CATGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATC119160.11189162100215867No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAA118570.1113376091156928No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCAAT118360.11114041844423886No Hit
ACGTGCCGTTGGTGGACCTCGTAAGGCGCTGTAGGCACCATCAATAGATC117320.11016385511894307No Hit
ATCCTGCCGTCCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG114330.1073562355587177No Hit
TCAGCGGCGGACCGGGCCCAAGTCCCCTGCTGTAGGCACCATCAATAGAT110640.10389131376031249No Hit
ATCCTGCCGTCCACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC109920.10321523145818465No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA109570.10288658033909472No Hit
TCTGCTTGAGCTGCTCCCGCGGCGAGAGCGGCTGTAGGCACCATCAATAG108700.1020696475573569No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT106620.10011652090676534No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA106560.10006018071492136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)