FastQCFastQC Report
Fri 14 Jul 2023
SRR8763789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13112294
Total Bases668.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC3944773.0084514578455916No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2867502.186878970224432Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAATA1901611.4502496664580584No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG1577421.2030084133256926No Hit
TGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATAG1271650.9698150453307408No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA1262300.9626843327338451No Hit
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA1204320.9184662882025068No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1134490.8652109234280438No Hit
ATGTACACGGATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA1097480.8369855038332729No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1004330.7659453029347878No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA977500.7454835896754602No Hit
ATGAGAATCGGTCGCCCTCGGCGTTCGACTGTAGGCACCATCAATAGATC771790.5886002861131698No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATC730180.5568667084493377No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAAT660200.5034969472161012No Hit
GCGCAGCGGAAGCGTGGTGGGCCTGTAGGCACCATCAATAGATCGGAAGA659680.5031003728256855No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC638320.4868103170963067No Hit
TGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCGG596860.4551911358912484No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA585560.4465732693302941No Hit
GAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGA570290.43492770982712864No Hit
GTCTAGTGGTAGAATAGTACCCTCTGTAGGCACCATCAATAGATCGGAAG535370.4082962142246048No Hit
TGTGCCGTTGGTGGACCTCGTAAGGCGCTGTAGGCACCATCAATAGATCG530190.4043457231816187No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA523830.39949531332961263No Hit
TAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAAT503010.3836170848518192No Hit
CCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCA465490.35500271729721744No Hit
TCAGCGGCGGACCGGGCCCAAGTCCCCTCTGTAGGCACCATCAATAGATC452490.34508835753682765No Hit
TGTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGG448350.3419310152746728No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA443050.33788900706466773No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAA427900.3263349647285212No Hit
ATGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCG411190.3135911992211279No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAAT403670.30785612342127167No Hit
AGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAGAT395840.30188462827328305No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCACTGTAGGCAC395250.30143466886877307No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA393520.3001152963775827No Hit
TGCTTGAGCTGCTCCCGCGGCGAGAGCGCTGTAGGCACCATCAATAGATC391640.29868152742761866No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC391050.2982315680231087No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCCTGTAGGCACCATCAATAGATCG376200.2869063186045096No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCG352160.2685723794783735No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAA323090.246402345768025No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA318240.24270352693434114No Hit
GTCTAGTGGTAGAATAGTACCCTGCTGTAGGCACCATCAATAGATCGGAA314150.23958431682511083No Hit
GTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT313290.23892844379480813No Hit
TGAGAGCCCCGTTGTGCTCGGACCCTGTCGCCTGTAGGCACCATCAATAG311220.23734977266373067No Hit
ATGTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCG309440.23599226801961581No Hit
ATGAGAATCGGTCGCCCTCGGCGTTCGAACTGTAGGCACCATCAATAGAT309310.23589312442201188No Hit
TGGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAA293300.22368320905556266No Hit
TCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTCTGTAGGCACCATCAA284090.21665926648685574No Hit
CGTGCCGTTGGTGGACCTCGTAAGGCGCTGTAGGCACCATCAATAGATCG283290.21604915204006253No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG278070.21206815527473685No Hit
TGCTCGCGGAGACGTCATCATCACGATTCTGTAGGCACCATCAATAGATC275980.21047423128248954No Hit
TGAGAATCGGTCGCCCTCGGCGTTCGACTGTAGGCACCATCAATAGATCG272810.20805665278707144No Hit
ATGAGAATCGGTCGCCCTCGGCGTTCGAATTCTGTAGGCACCATCAATAG271430.20700420536635314No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC265810.20271815137763077No Hit
CTCAGCGGCGGACCGGGCCCAAGTCCCCCTGTAGGCACCATCAATAGATC262730.2003692107574769No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTCTGTAGGCACCATCAAT255790.19507646793154576No Hit
TGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCA252770.1927732858949014No Hit
CTCAGCGGCGGACCGGGCCCAAGTCCCCTCTGTAGGCACCATCAATAGAT246250.18780085315353667No Hit
TCAGCGGCGGACCGGGCCCAAGTCCCCCTGTAGGCACCATCAATAGATCG241540.1842088043480416No Hit
TTGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATCG234270.17866438931280826No Hit
TAGTAAGCGCGAGTCATCAGCTCGCGTTGACTCTGTAGGCACCATCAATA231440.17650610945727727No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAAT227740.17368433014085866No Hit
TTGAGAATCGGTCGCCCTCGGCGTTCGACTGTAGGCACCATCAATAGATC226530.1727615320400839No Hit
TGTGTCTCGGTCGGATGTGGAACGGCTGTAGGCACCATCAATAGATCGGA224640.17132013665953494No Hit
TGTGGACCTCGTAAGGCGGATGAGCCTTACGGGACTGTAGGCACCATCAA213210.16260312650097686No Hit
TGATGCATTTATTAGATAAAAGGTCGACGCTGTAGGCACCATCAATAGAT208430.1589576926813874No Hit
GTGTCTCGGTCGGATGTGGAACGGTCTGTAGGCACCATCAATAGATCGGA203890.1554952931958359No Hit
GTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA202120.1541454149823059No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATACTCGGCCTGTAGGCACCATCAA192820.1470528345383348No Hit
TGGTGTGCACCGGTTGGCTCGTCCCTTCTGTAGGCACCATCAATAGATCG190910.145596186296616No Hit
TTGACCGACGCGGATTGTGATGGTGGCTGTAGGCACCATCAATAGATCGG189520.14453611244531278No Hit
AGATAAAAGGTCGACGCAGGCCTGTAGGCACCATCAATAGATCGGAAGAG187280.14282779199429177No Hit
TGGAGCCCGAGGGCGAGTTCTATCGGGCTGTAGGCACCATCAATAGATCG186250.14204226964404548No Hit
GTGTAGTGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG186130.14195075247702651No Hit
GGGTCCATAGCTCAGTGGTAGAGCATTTGACTCTGTAGGCACCATCAATA185640.14157705737836568No Hit
ATGGTGTGCACCGGTTGGCTCGTCCCCTGTAGGCACCATCAATAGATCGG182310.1390374559935889No Hit
TTGACCGACGCGGATTGTGATGGTGGCCCTGTAGGCACCATCAATAGATC178520.13614703880190604No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCAAT176590.13467513769901743No Hit
TCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCAT175140.1335693052642047No Hit
AGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAATA174370.13298207010916627No Hit
GTCTAGCGGTTAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA170460.1300001357504644No Hit
ACGTGCCGTTGGTGGACCTCGTAAGGCGCTGTAGGCACCATCAATAGATC166720.12714785071170615No Hit
TATGGTGTGCACCGGTTGGCTCGTCCCTTCTGTAGGCACCATCAATAGAT165300.12606489756864817No Hit
TCTGCTTGAGCTGCTCCCGCGGCGAGAGCGGCTGTAGGCACCATCAATAG164530.1254776624136097No Hit
CATGGTGTGCACCGGTTGGCTCGTCCCTCTGTAGGCACCATCAATAGATC163500.12469214006336343No Hit
TCAGCGGCGGACCGGGCCCAAGTCCCCTGCTGTAGGCACCATCAATAGAT162160.1236701983649848No Hit
GTCTAGCGGTTAGGACATTGGACTCTGTAGGCACCATCAATAGATCGGAA156950.11969682803024398No Hit
TTGGACCTCGTAAGGCGGATGAGCCTTACGGGCTGTAGGCACCATCAATA155080.11827068551086484No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA154690.11797325471805316No Hit
TATGTACACGGATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACC154440.11778259395343027No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA152800.11653185933750418No Hit
CCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTCTGTAGGCACCATCAA152630.11640221001756061No Hit
CGTGCTTCGGCACATGCGCGCTCCTGGCGTCGGCCTGTAGGCACCATCAA152090.1159903827659752No Hit
TGGTGGCTGTAGTTTAGTGGTAAGAATTCCACGCTGTAGGCACCATCAAT148630.11335163778359454No Hit
ATGTGTCTCGGTCGGATGTGGAACGGCTGTAGGCACCATCAATAGATCGG140650.10726574617683222No Hit
GTGCCGTTGGTGGACCTCGTAAGGCGCTGTAGGCACCATCAATAGATCGG138020.10525999493299952No Hit
TGAGAATCGGTCGCCCTCGGCGTTCGAATTCTGTAGGCACCATCAATAGA135970.1036965766630919No Hit
TCTGGAGCCCGAGGGCGAGTTCTATCGGCTGTAGGCACCATCAATAGATC133660.10193487119797648No Hit
GAGAATCGGTCGCCCTCGGCGTTCGACTGTAGGCACCATCAATAGATCGG133050.10146965893229667No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGACTGTAGGCACCATCAATAG132750.1012408660147492No Hit
AGCGCGTACACCGAAAGGGAATCGGGTTCTGTAGGCACCATCAATAGATC132460.10101969952778667No Hit
TAAGCGCGTACACCGAAAGGGAATCGGGTTCTGTAGGCACCATCAATAGA131910.10060024584561635No Hit
GCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCGGAAGAG131790.10050872867859734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)