FastQCFastQC Report
Fri 14 Jul 2023
SRR8763888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992405
Total Bases49.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGCCGT167231.6850983217537197TruSeq Adapter, Index 4 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATG163271.6451952579843914TruSeq Adapter, Index 4 (97% over 37bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93900.9461862848333089No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTAT86610.8727283719852277TruSeq Adapter, Index 4 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGCC65420.6592066746943033TruSeq Adapter, Index 4 (97% over 35bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58700.5914923846615041No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55320.5574337090200069No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAAACTCGTATG46230.4658380399131403TruSeq Adapter, Index 4 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAAACTCGTATGCCGT10900.10983419067820094TruSeq Adapter, Index 4 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAAGCTCGTATGCCGT10010.10086607786135701TruSeq Adapter, Index 4 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)