FastQCFastQC Report
Fri 14 Jul 2023
SRR8763889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939900
Total Bases98.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATG234851.2106294138873137TruSeq Adapter, Index 4 (97% over 37bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192530.992473838857673No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTAT182700.9418011237692665TruSeq Adapter, Index 4 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGCCGT177470.9148409711840817TruSeq Adapter, Index 4 (96% over 33bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122970.6338986545698232No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109970.5668848909737616No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGCC68450.35285323985772465TruSeq Adapter, Index 4 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30840.15897726686942626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)