FastQCFastQC Report
Fri 14 Jul 2023
SRR8763891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249506
Total Bases114.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATG593812.63973512406724TruSeq Adapter, Index 4 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGCCGT492012.187191321116725TruSeq Adapter, Index 4 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTAT336161.4943725422381626TruSeq Adapter, Index 4 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGCC216390.9619445335998215TruSeq Adapter, Index 4 (97% over 35bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127220.5655463910743069No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81880.3639910273633411No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81070.3603902367897663No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAAACTCGTATG26980.11993744404326995TruSeq Adapter, Index 4 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25450.11313595073762861No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGC25060.11140223675775926TruSeq Adapter, Index 4 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)