FastQCFastQC Report
Fri 14 Jul 2023
SRR8763892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028508
Total Bases52.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATG212692.0679469678407947TruSeq Adapter, Index 4 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGCCGT176341.7145223955477253TruSeq Adapter, Index 4 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTAT95470.9282377968863634TruSeq Adapter, Index 4 (97% over 38bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89680.8719426586861746No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAATCTCGTATGCC69300.6737915504789461TruSeq Adapter, Index 4 (97% over 35bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57630.5603262201169072No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53820.5232822690732595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29000.2819618320907567No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAAACTCGTATG22590.21963854437690322TruSeq Adapter, Index 4 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAAGCTCGTATG13690.13310543039042963TruSeq Adapter, Index 4 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAAGCTCGTATGCCGT12790.12435489077381995TruSeq Adapter, Index 4 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTCCGGAAACTCGTATGCCGT12750.12396597790197061TruSeq Adapter, Index 4 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)