FastQCFastQC Report
Fri 14 Jul 2023
SRR8836124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8836124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11433229
Total Bases438.2 Mbp
Sequences flagged as poor quality0
Sequence length19-42
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAGATCG4885244.272843655978551No Hit
CCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAA3739343.2705896120859648No Hit
CCGCCGGTGAAATACCACTACTCTAAAAAAAAAAAAAAA2340042.046700892634968No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGAT2036421.7811416179978552No Hit
CCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAAGA1863761.6301256626627525No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAA1093960.9568250579079628No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAGATC1030750.9015388391153542No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAA1024950.8964659065256193No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAGATCGG959730.8394216541976024No Hit
CCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAA927660.8113718355505694No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA895800.7835056920490265No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGG883620.7728525336105837No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAA872350.7629953008025991No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAAGATC565620.49471588472512884No Hit
ATGCGTGCATTTATCATAAAAAAAAAAAAAAAAGAT555840.48616187080657614No Hit
CCGATCGCACAAAAAAAAAAAAAAAAGATCGGAAGAGCG541080.4732521320092513No Hit
CCGCCGGTGAAATACCACTACTCCAAAAAAAAAAAAAAA490430.428951436204068No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATC477240.41741488778017133No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGA476000.416330329778228No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA449850.3934584009469241No Hit
ATGCGTGCATTTATCAGATAAAAAAAAAAAAAAAAG417580.36523365358990006No Hit
CCGCCGGTGAAATACCACTACTCAAAAAAAAAAAAAAAA415570.363475620054492No Hit
CCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAAGAT407760.3566446539293493No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATC403980.3533385013105222No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAAATCG399790.3496737448362138No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCAAAAAAAAAAAA380010.33237329541811855No Hit
CCGCCGGTGAAATACCACTACTCTAAAAAAAAAAAAAAAA379870.3322508453211249No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAA379300.33175229849765103No Hit
ATGCGTGCATTTATCAGTAAAAAAAAAAAAAAAAGA367130.3211078864947077No Hit
CCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAAAA359590.31451307412805257No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAA340760.2980435360824138No Hit
CCGGGCCACCCCTCCCACGGCGCGACCGCTAAAAA333490.2916848774742463No Hit
CCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGAT332790.2910726269892784No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAAGA328370.2872067024984805No Hit
ATGCGTGCATTTATCAGATAAAAAAAAAAAAAAAA321870.28152151942377784No Hit
AAAATAGGGAGTTGCTTTAAAAAAAAAAAAAAAAGATC321520.2812153941812938No Hit
CTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAAAA297140.259891584433409No Hit
CCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAA295020.25803734010750595No Hit
ATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAA292690.25599942063611253No Hit
CCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAAAG281820.24649204524810972No Hit
CTGAGAAGACGGTCGAACTTGACTATCCAAAAAAAAAAAAAA279840.24476025101920024No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA278140.243273356984278No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTAAAAAAAAAAAAA278050.24319463906478214No Hit
CTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAAA260540.22787963050508306No Hit
ATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAA234970.2055149949327526No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAA222410.19452947194532708No Hit
CTGAGAAGACGGTCGAACTTGACTATCCAAAAAAAAAAAAA218430.19104839061650913No Hit
CCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAA214570.18767226651368568No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCG204020.17844477706166822No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199830.1747800205873599No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGAAAAAAAAAAA198140.17330187298793717No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAA193690.16940970919064072No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAAAT189050.16535136311885296No Hit
CCGCCGGTGAAATACCACTACATAAAAAAAAAAAAAAAA171100.14965151139717398No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAATCGG167810.14677393411782447No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGA166520.14564564393838345No Hit
CCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAA165760.14498091484041822No Hit
CCCAAGTCCTTCTGATCGAGGTAAAAAAAAAAAAAA165110.14441239653294796No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAAAA161370.14114122965611903No Hit
AGCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAA158000.13819368089277317No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAGTTCG156560.13693419418083902No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAGATGG154460.13509744272593507No Hit
CGGAGCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAA150640.1317563043651098No Hit
CCGATCGCAAAAAAAAAAAAAAAAGATCGGAAGAGCGGT148530.12991080647470632No Hit
AGGTGGAGCACGAGCGCACGTGTTAAAAAAAAAAAAAAA144220.12614109277440344No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCAAAAAAAAAA142960.1250390419014611No Hit
ATGCGTGCATTTATCACAAAAAAAAAAAAAAAAGAT142030.1242256234000036No Hit
CCGGGCCACCCCTCCCACGGCGCAAAAAAAAAAAA141920.12412941260950866No Hit
ATGCGTGCATTTATCAGATAAAAAAAAAAAAAAAAA139620.12211773244461385No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAAGAT138350.12100693513617194No Hit
CCGCCGGTGAAATACCACTACTAAAAAAAAAAAAAAAAG137990.1206920634581884No Hit
CCGGGCCACCCCTCCCACGGCAAAAAAAAAAAAAA132880.11622263491792213No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAAATC131310.11484944454449394No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAAA127670.11166574202266044No Hit
ATGCGTGCATTTATCATAAAAAAAAAAAAAAAAGA126500.11064240906921395No Hit
CCGCCGGTGAAATACCACTACTCCAAAAAAAAAAAAAAAA126400.11055494471421852No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCAAAAAAAAAAAAAA124480.10887562909830635No Hit
CCGCCGGTGAAATACCACTACTCTGATAAAAAAAAAAAA123200.1077560853543649No Hit
ATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGAT122100.10679397744941521No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAGATCGGA119090.10416130036405288No Hit
ATGCGTGCATTTATCAAAAAAAAAAAAAAAAAGATCG118570.10370648571807668No Hit
CCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAA116420.10182600208567501No Hit
CCGCCGGTGAAATACCACTACTCTGAAAAAAAAAAAAAA115230.10078517626122944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)