FastQCFastQC Report
Fri 7 Jul 2023
SRR8874806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45102990
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG24344495.397533511636368No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA16982913.7653623407228656Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC15886513.5222742439026766No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA15400193.4144499067578447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG9939782.203796244993957No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG7757301.7199081479963967TruSeq Adapter, Index 4 (100% over 49bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT7601381.6853383777882573No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA7259501.6095385250512215No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC6901861.5302444472084888No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA5838571.2944973271173374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG5116371.1343749050783551Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC5028231.1148329634022045Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC4899151.0862140181837168No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA4713831.0451258331210416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4236450.9392836262074865No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC4155230.9212759508848527No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3579260.7935748827295043No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG3345880.7418310848127807Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT3028600.6714854159336221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA2907340.6446002803805246Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA2643900.5861917358472244No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2076140.46031094612574464No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG1848730.4098907855111158Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1845340.40913917236972536No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1802940.39973846523257106No Hit
ATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1772790.3930537642848069Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1770470.392539385969755No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1637530.36306462165812065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA1612680.3575550091025007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1606260.3561316001444693No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1604270.3556903877104378No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1534740.3402745582942506No Hit
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC1468960.3256901593442031Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT1467190.3252977241641851Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1373510.3045274825460574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1235290.2738820641380982Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1221490.2708224000227036Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA1217190.2698690264215299Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC1211810.268676200846108No Hit
GGGGGGGGATGCGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1200440.266155303672772Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1174770.2604638849885562Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTC1170790.2595814601204931No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA1126230.24970184903484227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1094600.24268901019644154No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1047340.23221076917516997No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG1043710.23140594448394664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA1024830.22721996923042131No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG1017320.22555489115023195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1006870.22323797158458897No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1004980.22281893062965447No Hit
GATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT969250.2148970611482742Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT959930.21283067929642802Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG862660.19126448157871573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC859530.1905705142829777No Hit
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA840410.18633132747961942Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG833100.1847105923576242Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT814820.18065764597868125No Hit
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA798930.17713459795015807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAG787660.17463587225591917Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG769910.17070043471619065No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT760740.16866731008299007No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA754780.16734588992880517No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC745520.16529281096441722Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA745000.1651775192731125No Hit
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA730850.1620402549808782Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA718180.15923112857928046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC712270.15792079416464408No Hit
GGGGGGGATGCGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT690720.15314284041922718Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT657130.14569544059052406No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC639260.14173339727587905No Hit
GGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCT633640.14048736015062416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA629670.1396071524304708Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA628090.1392568430607372Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCGGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC626250.13884888784535127TruSeq Adapter, Index 4 (100% over 38bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG622610.13804184600621822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC620390.13754963917026344No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG609390.1351107764695866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT593800.13165424287835462No Hit
CCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA574900.1274638333290099Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC565540.1253885828855249No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT552920.12259054222347565Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCCCGTCCTTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC540450.11982575878007201Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC532920.11815624640406322No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT525470.1165044712113321Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA520080.11530942848800046No Hit
ATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT514260.11401904840455145Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT498220.11046274315738269No Hit
TGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACTC492920.1092876547652384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT492420.10917679736975311Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT486920.10795736601941469Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAAC478710.10613708758554588Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT470930.10441214651179445Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGT458390.10163184303302288No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT457660.10146999123561432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT454420.10075163531286951No Hit
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC454020.10066294939648127Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG451640.10013526819397117TruSeq Adapter, Index 4 (100% over 35bp)
GCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT451350.10007097090458969Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)