FastQCFastQC Report
Fri 7 Jul 2023
SRR8874815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38452067
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG22544635.8630476223813925No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC17161054.462972042569259No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA11200252.912782295942634Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC8038812.0906054283115654No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG7460021.9400829609497974No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA7035591.8297039792425203No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC6137411.596119657234551No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT6097591.5857639070482221No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT4987871.2971656374155387Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4685901.2186340983958028No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4443341.1555529641618485No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC4224761.098708165675463No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3724190.9685279077455056No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT3313290.8616675925380032No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG3201480.8325898319068258No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA3034980.7892891687721235No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2862560.744448926503743No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA2659600.691666328366691Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC2546970.6623753152203755No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2424310.630475859724264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2307830.600183600012972No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1967130.5115797806136143No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1837050.4777506499195479No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA1782930.4636759839204483Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1774210.46140822546678706No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG1731050.45018386137733507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC1558030.40518758068324395No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG1514970.3939892229980771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTC1488950.38722235660309234No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC1425480.3707160918033353No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1336560.34759119711301867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC1320630.3434483769104012No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1316080.3422650855154289No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1268470.32988343643528967No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1208050.31417036696622835No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1159390.3015156506410956No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1094520.28464529618134704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT1077110.28011758119531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG1026350.2669167303801899No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC992740.25817597789996566No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG960870.24988773685430227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC960470.2497837112371618No Hit
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGT905950.23560501962092184No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA891290.23179248075272518Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG881290.22919184032421455Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA862270.22424542222918734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG854330.22218051372894987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA804470.20921372055239582No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA790850.20567164828876427Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT738150.1919662732305132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG736040.19141753810009746No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG732450.19048390818626212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT727280.18913937708472212No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT725910.18878308934601615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT694110.18051305278335233No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC691480.179829084350654Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA680910.17708020741771827No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT678890.1765548780511591No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA674680.17546000843075615No Hit
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC673030.1750309027600519Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG663080.17244326553368378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG651490.16942912327703996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA648650.16869054139534292No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC643300.16729919876608976No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA630690.1640197911857378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT619730.16116948927609015No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA615800.16014743758768546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC597100.15528423998637056No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCAGATCGGAAGAGCACACG561420.1460051549374446No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT541380.14079347151870925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC530830.13804979586663052No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC529290.1376492972406399No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG504110.1311008846416501TruSeq Adapter, Index 2 (100% over 49bp)
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG498260.12957950999097134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA492750.128146557114862No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA488180.12695806443903263Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACTC487470.12677341896860836Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT482700.12553291348420878No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC474860.12349401138825646No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT456020.11859440482094241No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT454930.11831093501423473Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC454570.11821731195880834No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT450890.11726027628111643No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC445540.11586893365186325No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC420170.10927110888473174No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG418470.10882900001188492No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGT412330.10723220678877939No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA405930.10556779691453257No Hit
GCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA385510.10025728915951385Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)