FastQCFastQC Report
Fri 7 Jul 2023
SRR8874817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24388204
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG339933713.93844745599143No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT14792876.065584001183523No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG9561473.9205305974970526No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT6782172.7809222852162465No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC6728112.758755831302707No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG4969922.037837636588574No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA4540091.8615925961583724Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4285341.757136359856593No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC2474711.014715966784598No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2404520.985935659714836No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2323420.9526818785015904No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC2268820.9302940060694918No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG1826770.7490383465711539Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1795060.7360361591202041No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT1592090.6528114985424921Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1408340.5774676970883137No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1265330.5188286927565473No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1141200.4679311358884812No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1016310.41672195295725756No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC984760.40378537099328843No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA957120.39245202311740546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA891360.3654881679684162No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT847630.34755736830805584No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC807030.33090997598675165No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG783070.3210855543114204No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT782600.3208928381934152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC744090.30510241754579387No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA727550.2983204503291837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC660170.27069233962451683No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT610930.25050225100626516No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC606070.2485094843392322No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC587650.24095665265060107No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC545830.22380901849107052No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA525180.21534181032764857No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG523560.2146775547719709No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC520400.21338184640410587No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC503620.20650147095702498No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT503300.20637025998306394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG482480.19783334598972518No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT478420.19616860675759476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA478100.19603739578363377Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT471930.19350748419194788Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA461810.18935793714043067Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC455520.1867788214335094Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG438440.17977543569834006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA428540.17571609619142106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG422900.17340350277535813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC405990.16646982287010556No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT394990.16195944564019554No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG387160.1587488771210869Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG380930.15619436347178334TruSeq Adapter, Index 10 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC372540.15275417574824288No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT371740.15242614831334034No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA361270.14813308925905327No Hit
GCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACT357120.1464314469404963Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT354010.14515624028731267No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA347730.1425812249233277No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG344030.14106409803690342Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA341970.14021942739202936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC338990.13899752519701739No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC335760.13767311442859836No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG329440.13508169769286824No Hit
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG325970.13365887869397844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC319000.13080093966739004Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACAC313090.12837763699204746No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTC303260.12434699988568244No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT293380.120295861064636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAA287740.11798326764857305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA283980.1164415387045311Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA274820.11268562457489695No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA266960.10946275502697943Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC264810.10858118129567884No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT250000.10250857340704546No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG244970.1004461009100957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)