FastQCFastQC Report
Fri 7 Jul 2023
SRR8874819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116625368
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT104828758.988503255998301No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT62261505.338589799776666No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG40067573.435579298665107No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC24094342.0659604692522815No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG23385422.0051743802428987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG20209581.7328631280288866No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC15303421.3121862132087765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT13893181.191265694441367No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA13437481.152191862751507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC10738810.9207953796124355No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT9200450.7888892577813774No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT8687350.7448936838510125No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA7238570.6206685667221218Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAA7091400.6080495282981658Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG6780310.5813752287581206No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG5632690.4829729669105953No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG5532010.47434019672289485TruSeq Adapter, Index 12 (97% over 49bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA5246590.44986696204894294Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT5010990.4296655252569064No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA4901890.4203107852144141Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA4511310.386820644373015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG4296070.3683649684175059No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT4236020.36321600288541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC4210900.36106209757040164No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4121270.35337680563631746Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC4056440.347817980732974No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT3879690.3326626159070298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT3847870.32993422151516816No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTC3647300.31273641940405283No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG3476140.29806036710640865TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG3379660.28978772439972067TruSeq Adapter, Index 12 (100% over 49bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT3160090.2709607741602153Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2706360.2320558594078777No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGA2572490.22057722467379481Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2472290.21198561191249576No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2380240.20409281795363768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2366930.20295155681738125No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG2149690.18432439158519953No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTC2116800.18150425043031804No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2083250.17862751781413458No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTATG2058540.17650876780084415TruSeq Adapter, Index 12 (97% over 43bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC1987270.17039774742661476Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTG1972220.16910729061965318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAAC1963660.16837331651549428Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG1933420.16578039865220404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTATG1859770.1594653060387342TruSeq Adapter, Index 12 (97% over 43bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1796060.1540025151303274No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT1752350.15025461698864692Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1750260.15007541069452401No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG1716520.14718238659705665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC1707460.14640554017372961No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1634500.14014961136071183No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1614400.13842614413015183Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA1549560.132866461780425No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1482170.12708813060294052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1481740.1270512604084559Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTGA1431650.12275631147418972Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA1407520.12068729335113439Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTCT1406410.12059211680258107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC1388380.11904614097337726Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCT1349830.11574068516551218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1341830.11505472805882165TruSeq Adapter, Index 12 (100% over 49bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC1327230.1138028563391114No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCT1303180.11174069778712295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG1286100.11027617936433863No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1280690.10981230087093916No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTG1276480.10945131594354326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC1272780.10913406078169889No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1202620.10311821695602282No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1201000.10297931064191797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCT1194040.1023825279590972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1193460.10233279606886214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)