FastQCFastQC Report
Tue 11 Jul 2023
SRR8874825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82677722
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG840138110.161601936734542No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT54734826.620262227350676No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG27385173.3122792134984076TruSeq Adapter, Index 7 (100% over 49bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT24075912.9120190321644324No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG21271122.572775287640363No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG20533842.4836001166069863No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC17724002.1437455666715155No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC14940711.8071022808296533No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC11889341.4380342990098347No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC9742391.1783573330673043No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC8551051.034262893697047No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG7148660.8646416261928456No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC6955640.8412955548049571No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT6640410.8031679924611371No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG5831000.705268584927872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5760790.6967765754358834No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT5467750.6613329283552346Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG4800850.5806703285801706No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4751290.5746759689387668No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4519910.5466901954555545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC4158880.5030230513608007No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3724270.4504562909945681No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC3260980.39442063969783786No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT3094900.3743330035145381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2667090.3225887138012825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT2570480.3109035829506768Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACT2478650.2997966005884874Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2464200.29804885045091106No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2391440.28924841446405597No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA2246410.2717068087579868No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG2191730.26509317709551794No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2167920.26221332029443195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA2124420.25695192714671067No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA2105670.25468408527269293Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC2077480.2512744606098364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC2076990.25121519434219536No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA2063670.24960411947489314No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG2019460.2442568507148758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC2010390.24315982000568423No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1930870.23354175142851663No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT1840140.2225678157896029No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1769410.21401291148297483No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1725860.20874547075692287No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG1707510.20652600950955086Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1703430.20603252711776454No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1694160.20491130609525016No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1621090.19607337512274467No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG1574220.19040437519553333No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1557170.18834215098475984No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1526730.1846603852970211No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1493720.1806677740830837Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTC1457380.17627239415232074Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCCGGGGCTACGCCTGTCTGAGAGATCGGAAGAGCACACGTCTGAACT1451810.17559869392627922Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1447820.1751160971754882No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC1362090.16474691936964592No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC1362040.1647408717913152No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1356760.1641022475195918No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA1347350.1629640932777514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTC1332970.16122480974983805No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1314750.15902107220612585No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT1271770.15382257387304407No Hit
GCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTC1255620.15186920607222343Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1252200.1514555517144026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1228370.14857327588198427No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAA1180840.14282444792080748Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTC1140800.13798154719357167No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1106050.1337784802537254No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACAC1091910.1320682251017995No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG1030970.12469743663232521No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC1030070.12458858022237236No Hit
CTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAAC1021520.12355444432782027Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA1019370.12329439845959955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC982220.11880104775987901No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC971850.11754678001408893No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA947940.11465482805634146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT936780.1133050085729261No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA911670.1102679147352415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTGAACTCC907120.10971758510714652Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT853730.10325998096561005No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGAGATCGGAAGAG841770.10181340022890327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)