FastQCFastQC Report
Mon 10 Jul 2023
SRR8874828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37279385
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG461007212.366276965137702TruSeq Adapter, Index 4 (100% over 49bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC6117231.640914945351164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG3801091.0196225071846008No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3727890.9999869901287267No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT3690350.989917081518378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG3599240.9654773006582592Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG3434320.9212383734334674No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT3020830.8103218440969453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC2524870.677283168700342No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT2431120.6521352216513229No Hit
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT2377190.6376687812848844Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAGGTGCGTGGCGGGGGCGGGCGGGCAGATCGGAAGAGCACACGTCTGA2319090.6220837602337056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG2228330.5977378650425698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGAGATCGGAAGAGCACAC2124580.5699074703083219No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC2030690.5447219689917095No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1993270.5346842497535836No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT1920250.5150970167560436Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC1853610.4972211853816795No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1807940.4849704468032399No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC1692390.4539747638004222No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA1613090.4327029536565584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1555440.4172386427512149Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGT1538680.4127428604307716No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1483020.39781235661478853No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGAGATCGGAAGAGCACACGTC1428160.38309644861362385No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT1377910.36961714899534953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1369440.36734511580596085No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGAGATCGGAAGAGCACACGTCTGAAC1330920.35701232732245985Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC1320120.3541152838224128No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACG1319810.35403212794417077No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTCTG1288330.34558778263107076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT1274860.3419745255990677No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1261270.33832907919484184No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT1227350.32923021664654606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT1222470.32792118217615446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1183090.3173577031917238No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT1137000.30499430181050463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC1113830.29877907052382974No Hit
TGCGGGCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCT1096080.29401772588254876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC1070300.28710237575002917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGA1069550.28690119217363697Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCAGATCGGAAGAGCACA1036390.2780061956494186No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTC980450.26300058329824916No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCAGATCGGAAGAGCACA939950.2521366701730729No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGAGATCGGAAGAGCACACGTCTGAA933540.25041722120684123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG932220.25006313811239106Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT915160.24548688236139088No Hit
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACAC911470.2444970591655415No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAGATCGGAAGAGCACA906030.24303780762477706No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG895120.24011125720019255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG881340.2364148442899474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG869680.2332871102889707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA860650.23086486002920917No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA800480.214724572307188No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG792570.2126027561881721No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGAGATCGGAAGAGCACAC791490.2123130518381674No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC783800.21025024956822652No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA774290.207699241819574No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAGATCGGAAGAGCACACG767510.20588054228898892No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACAC729720.19574357248650964No Hit
TGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCAC727070.19503272384992404No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG713470.19138459499801297No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA705430.1892279070590891Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGATCGGAAGAGCAC700780.18798056888545772No Hit
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA696870.1869317318405333Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC690020.18509425517615163No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT688810.18476967900623897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGAGATCGGAAGAGCACACGTCTGAACT683540.18335602907612344Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG676340.18142466674275876No Hit
GGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCT672160.18030340361033317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG665170.1784283726783583Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG641910.1721889993625163TruSeq Adapter, Index 4 (100% over 50bp)
CCGTGGCGCAATGAAGGTGAAGGAGATCGGAAGAGCACACGTCTGAACTC606770.1627628781966226Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA606600.16271727658597374No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG603930.16200106305401765No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC594000.15933739250258555No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA584500.15678906720161825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGTGCGTGGCGGGGGCGGGCGGGCGAGATCGGAAGAGCACACGTCTG582990.15638401760114873Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC582600.15627940214142483TruSeq Adapter, Index 4 (100% over 50bp)
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGAGATCGGAAGAGCAC574880.15420855252842824No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA574530.15411466685944522No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA564260.15135979308671535Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC552940.14832326230703646No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGAGATCGGAAGAGCACACG551600.1479638143172158No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT538850.1445436935185492No Hit
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGAGATCGGAAGAGCACACGTC522580.14017935113468208No Hit
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT516980.138677180430954Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCAGATCGGAAGAGCA511270.1371455028026884No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT510470.1369309069878701No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTC508910.13651244514897443No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG507140.13603765190868894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACGT506700.13591962421053888No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT499930.13410360712763905Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC495710.13297161420447254No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA491730.1319040000257515No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT490210.13149626797759673No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC488440.13102147473731124TruSeq Adapter, Index 4 (100% over 50bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCAGATCGGAAGAGCACAC485680.13028111917618812No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC485190.13014967923961193No Hit
CGGGCGTGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTGA483550.1297097578192344Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGA472690.12679661963307604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT470370.12617429177010298Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT461230.12372253458580393No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACG445450.119489632138513No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA441220.11835495676766127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCGCGCGGGCGGGGCCGGGGGTGGGGAGATCGGAAGAGCACACGTCTG441020.1183013078139567Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACGTC431090.11563763726252457No Hit
GCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCAGATCGGAAGAGCAC428450.11492947107362421No Hit
GGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGAGATCGGAAGAGCACAC423320.11355337541110186No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA417100.1118848929508896No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT416400.11169712161292361Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGAGATCGGAAGAGCACACGTCT413360.11088165751661408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACAC408920.10969065074437252No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCAGATCGGAAGAGCACACGT403850.10833064976796157No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACG387350.10390461108733418No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC382160.1025124207387005No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCAGATCGGAAGAGCACAC373410.10016527901412536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)