FastQCFastQC Report
Fri 7 Jul 2023
SRR8874832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47505780
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC10333502.175208995621164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG8228561.732117649683891TruSeq Adapter, Index 11 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG7764941.634525314603823No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG7270921.5305337582079486No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG6882441.448758445814383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC6456301.3590556770144602No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC6424371.352334389625852No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG5870971.2358433015940375No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT5805131.221983935428489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA5742061.2087076562052028No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT5477601.1530386407717124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG4213770.8870015396021284Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG4207690.8857216953389672No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA4171690.878143670096565Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT3670530.772649138694281Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA3651410.7686243652877608Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA3636060.765393179524681Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG3484050.7333949679386382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG3280820.6906149104382667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTCTG3223510.6785511152537649Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC3104300.6534573266663551No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT3040450.6400168568961503No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2913570.6133085279307066No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC2701530.5686739592529583No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT2552540.5373114597844725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA2537940.5342381495472761No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT2535910.5338108331238852Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC2400930.5053974484789009No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC2386630.5023872884520578No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCAGATCGGAAGAGCACACGTCTGA2363170.4974489420024258Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT2311200.4865092205622137No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGA2276570.47921958128042524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT2213950.4660380273726692Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2207170.46461083261868347No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2192310.46148279219918087No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC2184720.459885091877241No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2029230.42715433785109935Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG1934170.4071441411971344No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA1896680.39925246990997726Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1847930.3889905607275578No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1828970.38499946743322605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA1777280.3741186861893437Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCAGATCGGAAGAGCACA1653550.34807343443261013No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1644860.3462441833393747Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC1608450.3385798528094897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC1558050.3279706174701268No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1557810.3279200973018441No Hit
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG1484480.3124840808844734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT1452460.30574384843275915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1426220.3002203100338527No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT1412030.29723330508413925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1334200.28085003551146825No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG1295180.27263629815150914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1229190.25874535688078376No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1223960.2576444382136237No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT1203450.25332706883246625No Hit
GGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCT1203330.253301808748325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGAGATCGGAAGAGCACAC1159280.24402925286144128No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC1106240.23286429567096886No Hit
GCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC1093060.230089896429445No Hit
GGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGA1092980.23007305637335076Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT1090940.22964363494294798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGT1075750.22644612929205668No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1067230.22465266331802153No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA1063980.22396853603919353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1051500.22134148728849418No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1046770.220345818971923Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTC1014450.21354243630985534No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1008240.21223522695554098No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT999310.21035545569402292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGTGCGTGGCGGGGGCGGGCGGAGATCGGAAGAGCACACGTCTGAAC972970.20481086722499872Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT965400.2032173769170825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGAGATCGGAAGAGCACACGTCTGAACT961780.20245536437881873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG959730.2020238379414042No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG958710.2018091272262028No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA940670.19801169457695467No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCAGATCGGAAGAGCACAC938350.19752333295022206No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG923070.19430688223622475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC878130.1848469807252928No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAA865810.18225361208678187Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA852720.179498157908364No Hit
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT849820.17888770587494826Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGAGATCGGAAGAGCACACGTCTGAACTC846460.17818042351899074Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGCGCGCGCCGCGGCTGGACGAGGAGATCGGAAGAGCACACGTCTGAA837800.1763574874467907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT834480.17565862511888028No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT832940.17533445403906642No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGAGATCGGAAGAGCACACGTCTGAA825450.17375780378724442Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGAGATCGGAAGAGCACACGTC820280.172669515162155No Hit
CGGGCGTGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTGA810550.17062134333969467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC803900.16922151367686206No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA765450.16112776171657428No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT760140.16001000299331997No Hit
GGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTGA703120.14800725301215978Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT701660.14769992198844015No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC692480.1457675255516276No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG692170.1457022703342625Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCAGATCGGAAGAGCACACG676080.1423153140523111No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC671700.1413933209811522Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG671590.14137016590402263Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTC667550.14051974307126416No Hit
TGCGGGCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCT667420.14049237798011105Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA658930.13870522702711122Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG655380.13795794953792992No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGT651120.13706121655091233No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACGTC637670.13422998212007042No Hit
GGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC636790.13404474150303394Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGGGCGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC629950.13260491670697755No Hit
GGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTG614850.12942635611919223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG613580.12915902022869638Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC594950.1252373921657533No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC591910.12459747003417268No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGAGATCGGAAGAGCACAC586660.12349234135298905No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG582410.12259771337298324Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAA580760.12225038721603981Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC573350.12069057702031205No Hit
CGCGGGCGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG570120.12001065975550765No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT559000.1176698919584101No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA553160.11644056786353156Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACAC550420.11586379594230428No Hit
GATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAAC547970.11534806922441859Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT546390.1150154781165576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTC542930.11428714569048229No Hit
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTC540900.11385982926709129No Hit
GCCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACG532550.11210214841225637No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCT532380.11206636329305614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGAGATCGGAAGAGCACACGTC518190.10907935834334265No Hit
TCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAA502640.10580607244002731Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCT495760.1043578276159238Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACG482010.10146344297472855No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC478380.100699325429453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)