FastQCFastQC Report
Fri 7 Jul 2023
SRR8874833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42993134
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC20312504.724591605720113Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG16203753.7689157529199893TruSeq Adapter, Index 7 (100% over 49bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG6706821.5599746694437302No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG6012551.398490745057106Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT5988621.3929247400294196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG5699461.3256674891390798Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG5382381.2519161780576404No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT5333551.2405585505815881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC5229841.216436094191226No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA5205751.2108328739188914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA4816541.1203044653595153Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTCTG4231750.9842850721233767Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG3921620.9121502982313408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA3854270.896485006187267No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT3765460.8758282194547622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGA3444550.8011860684545584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG3329640.7744585449388267No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA3317470.7716278603927781Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC3169510.7372130629044163Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC2968250.690400937042645No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG2960340.6885611083853529No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2956090.6875725784493868No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2919050.6789572493133439Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG2860310.6652946026218978Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA2581510.6004470388225246Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA2449630.5697723734213002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC2440830.5677255349656528No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC2320400.5397140855095607No Hit
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT2305990.5363623875384381Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA2107440.49018059488289456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT2078290.4834004424985627No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT2027530.4715939061339422No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1954070.4545074569348678No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1918470.44622706500065795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG1906070.44334288354042767No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT1856620.4318410469913637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT1836910.4272565940412718Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAA1819520.42321176213857775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT1794830.4174689846988126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1785350.41526398145341065No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1752520.4076278784421717No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGAGATCGGAAGAGCACACGTCTGAA1709670.39766117073484336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1510360.3513026056672212No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1483010.3449411247851808Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT1422900.3309598225614351Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1408870.3276965107963518No Hit
GGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC1390080.32332604550298655Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGGTGCGTGGCGGGGGCGGGCGGGCAGATCGGAAGAGCACACGTCTGA1384770.32209096457122666Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG1373330.3194300745788851Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGAGATCGGAAGAGCACACGTCTGAAC1353560.31483166591205003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT1350810.31419202889466025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1337540.3111054895416557No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC1264920.2942144203769839No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG1252010.2912116153244376Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTGA1232850.2867550897778236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGCGCGCCGCGGCTGGACGAGGAGATCGGAAGAGCACACGTCTGAAC1225760.28510598924935315Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1139810.26511442501493376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA1135820.26418636985152094Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT1105510.2571364069434901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCAGATCGGAAGAGCACA1100790.25603855722637014No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1041240.24218750835889283No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT1034230.24055701545274646Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC1005350.23383966379375834No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGAGATCGGAAGAGCACACGTCTGAAC971530.22597329145625905Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG936880.21791386503714755TruSeq Adapter, Index 7 (100% over 50bp)
GGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGA930070.21632989118681137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGTGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTGA927200.21566234273593549Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC912970.21235251191504206No Hit
CCGTGGCGCAATGAAGGTGAAGGAGATCGGAAGAGCACACGTCTGAACTC901940.20978698598711135Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT893110.20773316967309247No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT880270.20474664629007971No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG858080.19958535704794164No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT848230.19729429354929093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTC804180.18704847150710158No Hit
CCGTGGCGCAATGAAGGTGAAGGGAGATCGGAAGAGCACACGTCTGAACT796130.18517607951074236Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGAACT752160.17494886509087706Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC751030.17468603242554961No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG749550.17434179141255438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT748170.17402080992746424No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG730280.16985968038524477Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTC712480.16571948441813988No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT682140.15866254365173751No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT678860.15789963113645075No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA674140.15680178141933082No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG650500.15130322902256904Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT647620.15063335461890265Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC643680.14971692921944235TruSeq Adapter, Index 7 (100% over 50bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCAGATCGGAAGAGCACAC629490.14641640220971097No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG612310.1424204153156176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG608090.14143886323802307Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCT601110.13981534819024824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGCGGCGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACT591890.13767081971739953Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCAGATCGGAAGAGCACACGTCTGA588110.13679160956258735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT579390.13476337872926408No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGAGATCGGAAGAGCACACGTC578760.13461684370346205No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC577770.13438657437720172No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG577200.13425399506814276No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT566010.13165125389556387No Hit
CGGTGGGGGGCGGGGCGGACTAGATCGGAAGAGCACACGTCTGAACTCCA560360.13033709056892664Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTG553260.1286856640876657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG551050.12817162852096337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCT544420.12662952182085632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT540960.12582474215534042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC540850.12579915667464484No Hit
GGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAACT531240.1235639160429663Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAGGTGCGTGGCGGGGGCGGGCGGGAGATCGGAAGAGCACACGTCTGAA531050.12351972293994663Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGAAC516480.12013080972417597Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCT512970.11931440029470752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT500320.11637207001471445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAAC498960.1160557404352053Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT487600.11341345806518781No Hit
TGCGGGCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCT486780.11322272993636612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGCCCGGTGGGGGGCGGGGCGGAAGATCGGAAGAGCACACGTCTGAA485420.11290640035685698Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA481840.11207370925785498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGAGATCGGAAGAGCACACGTCT481820.11206905735227396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC478260.11124101815885298TruSeq Adapter, Index 7 (100% over 50bp)
GATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAAC475380.11057114375518659Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAA472440.10988731363477712Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAACTC466680.1085475648274443Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG459750.10693567954362201No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA448150.1042375743066323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC444260.10333277867112456Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT441270.10263731878676256TruSeq Adapter, Index 7 (100% over 41bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCAGATCGGAAGAGCACACG434250.10100449992782567No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTC430920.10022995764858639No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)