FastQCFastQC Report
Fri 7 Jul 2023
SRR8874834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42730093
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6209911.4532872652535533No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC5051931.182288557153386No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4945311.1573365871213994No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG3798970.8890619545340095No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG3711050.8684862913825158No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3250140.7606208579981326No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA3173540.7426943816855255No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA2772380.6488120678791875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA2708450.6338507149984438Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2415710.5653416200147283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2359580.5522056785600724No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC1962950.459383507543501No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1924010.4502704920394159No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1846220.43206552347077737No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA1732810.4055245093896706Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1595500.3733902474773458No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATG1583180.3705070335325505TruSeq Adapter, Index 7 (97% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1532490.35864419953403803No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1301970.3046962710799623Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1223400.28630876136871497TruSeq Adapter, Index 7 (100% over 49bp)
TCGGGCCCTCCGTCTCCCGAAGTTCAGACGTAGATCGGAAGAGCACACGT1208190.28274920908784357No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC1147320.2685039791511804No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1077160.25208463740062537No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1018490.2383542670969614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGTGGTATG998940.2337790371764461TruSeq Adapter, Index 7 (97% over 43bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT989740.231625987802086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG970060.2270203343578026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCCCTCCGTCTCCCGAAGTTCAGACGTAGATCGGAAGAGCACACGTC948610.22200045293606077No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC914390.21399204537186473No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC908150.2125317162309944No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGTGGTATG887510.20770139676503865TruSeq Adapter, Index 7 (97% over 43bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA871470.20394760198626294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA833390.19503584979325927Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATG808180.18913602645330072TruSeq Adapter, Index 7 (97% over 49bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG807490.1889745477502237No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC806620.18877094416808315No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGGATG680040.1591477931021587TruSeq Adapter, Index 7 (97% over 45bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC646790.15136639183069414No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT629650.14735516723541883Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA629280.14726857720623263No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG609450.1426278196960629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT607060.1420684949129411Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG603400.14121195570531522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT592260.1386048937454922Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT584130.13670225337445438No Hit
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT542170.12688247601052496No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA540540.12650101182789375No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC527170.12337206942189431No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT523180.12243830126931855No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG517160.12102945809174812No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT510260.11941467106097803No Hit
GACCGTGAAAGCGGGGCCTCACGATCCTTCTGAAGATCGGAAGAGCACAC507620.11879683950137904No Hit
TCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGAGATCGGAAGAGCACACG500950.11723587870496795No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG469360.10984296242931182TruSeq Adapter, Index 7 (100% over 49bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC457330.10702761634523006No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC441690.10336743240881782No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC441150.10324105777162712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)