FastQCFastQC Report
Fri 7 Jul 2023
SRR8874835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8874835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26404155
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC19915907.542714394760976No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG7014832.6567144451318363Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT6079592.3025126159121547No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA4930011.8671341688457743Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4069681.5413028744907762No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4034111.527831509851385No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA3382371.2809991457783823Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA3282171.2430505729117254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2298630.8705561681485356No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2031370.7693372501411235No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1603040.607116569342969No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT1218090.4613251209894806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG990420.3751000552753913No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA890620.3373029737175835No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG739500.280069557234458No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT712790.26995372508607074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC663790.2513960397520769No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT651180.24662027624061442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG588890.2230292921701149No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC586590.22215821714423353No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT548340.2076718607355547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT547260.20726283420166258No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG533970.20222953546515687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG529800.20065023857040684No Hit
TAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC514390.1948140358970018Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA468340.17737359896576882Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGAGATCGGAAGAGCACA454850.1722645545748387No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA447740.16957179656004898Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT444780.1684507608745669Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC443130.16782585922556506Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT442540.1676024095450129Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT439190.1663336698334031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA424960.16094436652110247Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCTCGCTGGCCCTTGAAAATCCGGAGATCGGAAGAGCACACGTCTGA418130.158357652422507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG389250.1474199799236143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC383390.14520063224897747No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC380490.14410232025982275No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT362240.13719052929359035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC352140.1333653737451549No Hit
GCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGAGATCGGAAGAGCACAC349420.13233523284498216No Hit
GACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTGAAC347280.1315247543426404Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTAGATCGGAAGAGCACACGTCTGAAC344520.13047946431158278Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG337990.128006368694624No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA328160.12428346977966158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAGACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA321600.12179901231453914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG320300.12130666556078012No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT313620.118776760703003No Hit
CGCCGCCGGGCGCACTTCCACCGTGGCGGTGAGATCGGAAGAGCACACGT311870.11811398622678894No Hit
CCGCCGCCGGGCGCACTTCCACCGTGGCGGTGAGATCGGAAGAGCACACG304750.11541744092927798No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC303350.11488722134830674No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA301470.11417521219671677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG301300.11411082839045597Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC295130.11177407495146123No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG286180.10838445691596646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT283340.10730886862313906No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG282190.10687333111019837Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA281640.1066650305605311Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA281110.10646430457630628No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA278880.10561974052947348Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG278850.10560837868130982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)