FastQCFastQC Report
Fri 14 Jul 2023
SRR8887170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8887170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110330347
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG54531614.942575772013116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT44072573.994600868970348Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT21792261.975182766351673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC16380541.4846812726873777No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC15780931.4303344844913792Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG11833721.0725716289100404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC11035541.0002270726113098No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT8603400.779785456489138No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC7480350.6779956923365791Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC5961360.5403191562517247No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT5883290.5332431339131019Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG5759710.5220422265145237Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAAC5508420.4992660813438754Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA5344310.4843916606189954Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCGGCGTCCGGTGAGCTCTCGCAGATCGGAAGAGCACACGTCTGAACTC4664540.42277941897527066Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG4639420.42050262019025464Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCGGCGTCCGGTGAGCTCTCGCAGATCGGAAGAGCACACGTCTGAACT4579360.41505896831811834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4566030.41385077851699315No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC4355780.39479437148874374Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTGAAC4249670.3851768906337256Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT4155180.3766126104905661Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGTCT4095690.3712206216481853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTGA3931840.3563697665158254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT3832250.34734323821169527Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGTC3743490.3392983074729204No Hit
AAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG3594010.32574990451176594Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACGT3571700.32372779539975527No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG3303900.29945523510408245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3269440.29633188772623No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3232830.29301367102561543No Hit
CGGCGGCGTCCGGTGAGCTCTCAGATCGGAAGAGCACACGTCTGAACTCC3184450.2886286580789962Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC3067190.27800057585244425Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2979960.2700943195619606No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA2916960.2643841952205589Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2907870.26356030585129947No Hit
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC2823830.25594318125365817Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC2786710.25257873973694656Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2751510.2493883210573062Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTC2596050.2352979094681901No Hit
TTTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2594690.23517464329193127Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGCGGCGTCCGGTGAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG2592500.23497614849339685Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2553990.23148572169359713Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGGGCCCGTGCCTTGGAAAGCGTCGCAGATCGGAAGAGCACACGTCTG2551680.23127635046774575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTGA2543840.23056575721637132Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACT2477280.2245329655312332Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGTTTACTTTGAAAAAATTAGATCGGAAGAGCACACGTCTGAACTCCAG2400500.21757386478626775Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGGGCCCGTGCCTTGGAAAGCAGATCGGAAGAGCACACGTCTGAACTC2358640.21377980439053634Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2344890.21253354709380184No Hit
CGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTC2211240.20041992616954243Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA2142040.19414785308343135Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAAC2135220.19352970946425102Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGCGTCCGGTGAGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCA2128910.19295779066116778Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGAACT2107490.19101634838509118Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2102640.19057675944769756No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA2097730.19013173229664546Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAGATCGGAAGAGCACA2057000.1864400915914821No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2054990.18625791143392306No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2030290.18401918014451638No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGATCGGAAGAGCACACGTCT2017700.1828780616451791Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1983500.17977827985984673Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1972810.17880937145969458No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC1962790.1779011897787288Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1924790.17445698779502616Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1888730.17118862138628096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCT1875600.1699985589640174Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCC1809310.16399023924034245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCT1804120.16351983375888413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1792740.1624883859016595No Hit
AGATCCCCGAATCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1765520.1600212496385967Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCGGCGTCCGGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1724100.15626706947636085Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1716800.1556054201479127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1689890.15316638132208538Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGCGGCGTCCGGTGAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA1684750.15270050768534246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCT1672560.15159564394372838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGAACGTCTGCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1658020.1502777835004906Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTCCCGACCCGGGGAGGTAGTGACGAGATCGGAAGAGCACACGTCTGAA1653730.1498889512239094Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1624780.14726501313369386No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1612890.14618734046037216No Hit
GTACAGTGAAACTGCGAATGGCTCATAGATCGGAAGAGCACACGTCTGAA1576490.14288815750756226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGGGCCCGTGCCTTGGAAAGCGTAGATCGGAAGAGCACACGTCTGAAC1574390.14269782002951553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGAA1553410.14079625798693446Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1520760.13783696338777943Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAACT1515980.13740371903298737Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1504600.13637227117576273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1493400.13535713795951354Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCGGTGCGCCGCGACCGGCTCAGATCGGAAGAGCACACGTCTGAACTCC1485950.1346818930969192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1472090.13342566574181083Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAA1470850.13331327599286896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1469570.13319726076815475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1462120.13252201590556043No Hit
TGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAACT1430050.129615290705104Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG1406850.12751251475715925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTGACTCTAGATAACCTCGGGCCAGATCGGAAGAGCACACGTCTGAAC1399850.12687805649700348Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1339510.12140902629446093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT1333760.12088786415219013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1328060.12037123385463476No Hit
AGTGAAACTGCGAATGGCTCATAGATCGGAAGAGCACACGTCTGAACTCC1326500.12022984029951432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1318650.11951834067919682Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGT1317990.11945852032895356No Hit
GCGTTTACTTTGAAAAAATTAGAGTGAGATCGGAAGAGCACACGTCTGAA1304210.11820954392538982Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1292540.11715181136881588Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1290350.11695331657028142Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1255010.11375020872543798Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACT1230820.11155770225212833Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGCACACGTCTGAACT1230020.11148519273668196Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGA1218610.11045102577262808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA1216750.11028244114921527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1204840.10920295573800742Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCAGATCGGAAGAGCACACGTCTGAA1183920.10730683190908481Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACT1183790.10729504911282477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACGTCTGAACTCCAG1154740.10466204733317842Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATAAACGATGCCGACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1141760.10348558044506104Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAACGTCTGCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA1133200.10270972862978488TruSeq Adapter, Index 3 (100% over 36bp)
CGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1131470.1025529268026321Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1108740.10049275019501207No Hit
TTTGGTGACTCTAGATAACAGATCGGAAGAGCACACGTCTGAACTCCAGT1105960.10024077962883593Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)