FastQCFastQC Report
Fri 14 Jul 2023
SRR8887171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8887171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76488071
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC17384102.272785778582388No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT14101691.8436456581575968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA13041361.7050188126721093No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT12221401.59781778259253No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG11990461.567624839172634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC10136561.325247174817626No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT9536071.2467395079162082Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA8772641.1469291727856492No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC8538741.116349240916273Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC8147191.06515825193186No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC6397300.8363787864384761No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC5233630.684241337449862No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATAAGATCGGAAGAGCACACG4279010.5594349477057671No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC4002170.5232410685321114No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT3812170.49840059373441387Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA3617700.47297571408226513No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC3548220.4638919446667703Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3358320.43906454380317683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACAGTGAAACTGCGAATGGCTCATAGATCGGAAGAGCACACGTCTGAA3243680.4240765857462924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC3187070.41667543164998894Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG3110900.40671701604293303No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2988710.3907419759611927No Hit
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG2898300.37892183213771985Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2864050.3744440097070823No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2827360.36964718328430585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2738870.35807805899563083No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC2729020.3567902764863818No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTC2532780.3311339882005914No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT2467070.32254310610081927No Hit
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA2447600.31999761113076053Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATAAGATCGGAAGAGCACACGT2414530.3156740611225507No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2393970.31298606027075776No Hit
ATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACGTCTGAACTCCAG2283510.2985445926594227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATAAGATCGGAAGAGCACAC2269120.2966632535418497No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA2260320.29551274734069316Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGGTGACTCTAGATAACAGATCGGAAGAGCACACGTCTGAACTCCAGT2235450.2922612599290156Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2152130.28136805803352005Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAAC2098090.2743029040436907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2031490.2655956639303925No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1993100.26057658062784717Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1967900.25728194923362624Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1961640.2564635209587126No Hit
AGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGAACTCC1941010.25376636835304683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1915560.2504390521235658No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA1901250.248568172153276No Hit
TTTTGGTGACTCTAGATAACAGATCGGAAGAGCACACGTCTGAACTCCAG1844670.24117094023720378Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT1840130.24057738362887987Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1754590.22939394039627437No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCT1721120.22501809465164838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA1691510.22114690276343876No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATAAGATCGGAAGAGCACA1664580.21762609230921776No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC1664050.21755680045846626Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAA1654750.2163409245867895Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGAA1636400.21394185767869608Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGA1617420.2114604249857471Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCCGACCCGGGGAGGTAGTGACGAGATCGGAAGAGCACACGTCTGAA1525550.19944940172435516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGAAC1502520.19643847470019213Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1496050.19559259116366Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG1486030.19428258296643408No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1479990.19349291734654936No Hit
AGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1474240.1927411661355664Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1464840.1915122163297856No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGATCGGAAGAGCACAC1451830.18981129750284853No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC1449880.1895563557878195No Hit
GTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACT1445210.18894580306516032Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1408920.1842012723788001Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1404690.18364824496619872No Hit
GCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAAC1403870.1835410387065455Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT1385900.18119165274804747No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGTC1374180.17965938767105266No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG1373660.1795914032137116No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1355600.17723025071451992No Hit
AAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG1331190.17403890339972097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGA1311300.17143849790642518Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1296390.16948917433151112Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1293160.16906688625995026No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1290100.16866682387636628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC1207360.1578494507986742No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1199520.1568244543649166Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGATCGGAAGAGCACA1194470.156164220692662No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACA1190930.15570140342537858No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA1180980.15440054698202546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1167120.15258849971520394Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT1141310.14921411732294831No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATAAGATCGGAAGAGCACACGTC1141140.14919189163497143No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCGAGATCGGAAGAGC1132790.14810021813728314No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACGTCT1115690.14586457540549036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACAC1082630.14154233279069098No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1060210.13861115676456268No Hit
CGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1053520.13773651057300162Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCT1049840.13725538979797253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1033840.13516356034132432Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTG996940.13033927865692937Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACG985970.12890506808571497No Hit
TTGGTGACTCTAGATAACCTCGGGCCAGATCGGAAGAGCACACGTCTGAA965670.12625105946259255Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACGT962040.12577647565461547No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCAC958540.12531888796097368No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGAGATCGGAAGAGCACAC921100.12042400703241686No Hit
TCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAA920240.12031157119912202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGGGCCCGTGCCTTGGAAAGCGTCGCAGATCGGAAGAGCACACGTCTG895130.11702870634559472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT891920.11660903306085468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGAGATCGGAAGAGCACACG884530.11564286933056528No Hit
ATTCGAACGTCTGCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA843370.11026163805333776Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTG835250.10920003460408878Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA822780.10756971502131357Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA818350.10699053974050411Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGTTTACTTTGAAAAAATTAGAGTGTAGATCGGAAGAGCACACGTCTGA799410.1045143366211968Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC799020.10446334827819098Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCAC793160.10369721573969357No Hit
AAATTAGAGTGTTCAAAGCAGGCCCAGATCGGAAGAGCACACGTCTGAAC786660.10284741002293024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCCCCGAATCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC782090.10224993123437509Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGA777980.1017125925426986Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT776820.10156093490709159Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGATCGGAAGAGCACACGT769980.10066667781437448No Hit
ATTACCCACTCCCGACCCGGGGAGGTAGTGACGAGATCGGAAGAGCACAC768660.100494101884201No Hit
AGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG767130.10029407069240902Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)