FastQCFastQC Report
Fri 14 Jul 2023
SRR8887173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8887173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38551678
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC13009553.3745742532919056No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT10962012.843458590829691No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA8370382.1712102907686663Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5977461.5505057912135498No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA5233821.3576114637604102Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA4949581.2838818585276626Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4869341.2630682379117195No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4317061.119811179165794No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC4124371.0698289189902448No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC3433460.8906123359922233No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3219460.8351024305608695No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2682670.6958633551566809No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2661320.6903253342176182Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2642720.6855006415025566No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA2470920.6409370819086008No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG2393930.6209664855573861No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG1845070.47859654773003657No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1785750.46320940945813044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1678880.4354881777130427No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1574210.40833760854715584No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1520960.39452498020968113No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1482390.38452022762796473No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT1462560.3793764826527136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1422140.36889185472030556No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1391670.36098817799837407No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1295210.33596721782123207No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACGT1275710.33090907223286103No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT1237250.3209328527801047No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA1191750.3091305130739056No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1177730.3054938360919076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1106770.2870873739918662Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1106650.2870562469420916No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1084130.2812147372677267No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1038350.26933976777872026No Hit
AACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGAA1004830.26064494520835124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCATGGTGACCACGGGTGACGGGGAATCAGATCGGAAGAGCACACGTCT990850.2570186439096114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT900660.23362407208319183No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT888320.23042317379803806No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC886250.22988623218942633No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG842600.21856376783391893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT839570.21777780982711054No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTGA830240.21535768170713607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC810170.21015168263233572No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT776860.2015113323990722No Hit
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGAA767630.19911714348724327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC767030.19896150823837033No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA725990.18831605721546024Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG706030.18313859126961998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT696190.18058617318810352Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG687820.1784150614663258No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC676180.17539573763819047No Hit
GGACCGGCGCAAGACGGACCAGAGCGAAAGCAGATCGGAAGAGCACACGT671630.17421550366757058No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC669120.17356442954311874No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA667470.17313643260871808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACT660460.1713180941177191Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGAGCACACGT658120.17071111664711455No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC655710.1700859817308082No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG643720.16697587067416364No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA609750.1581643216671399No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGATCGGAAGAGCACACGTC606110.15722013449064395No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG597470.15497898690687342No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT594560.1542241559498396No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT591110.1533292532688201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACG582160.15100769413979853No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC577710.14985339937732411No Hit
CATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACG574530.14902853255829746No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG573850.14885214594290813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA571760.1483100164926673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG561950.14576538017359453No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT555380.14406117419843567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGT553750.14363836510566413No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG541720.1405178783657614No Hit
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC538050.13956590942682182Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTG531360.1378305764018884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG529200.13727028950594575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCAC529130.13725213206024392No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG522250.13546751453983402Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA521910.13537932123213936No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCT511150.1325882624356844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT510200.1323418399583022No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC497870.129143535593963No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC496520.12879335628399885No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACAC488550.12672600139480308No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAGATCGGAAGAGCACACGT476240.12353288487209299No Hit
ATGGTGACCACGGGTGACGGGGAATCAGATCGGAAGAGCACACGTCTGAA470040.12192465396707246Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC463640.12026454464576095Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCATATGCTTGTCTCAAAGATTAAGCCATGCAGATCGGAAGAGCACACG458140.11883788819775885No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG454560.11790926454615022Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA449330.11655264396014098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA448560.11635291205742068Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG447430.11605979900537663No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC447090.11597160569768196No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG446370.1157848433990344No Hit
AAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTGA443180.1149573826591932Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGT439500.11400281979943908No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC432870.11228305029939292No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT432520.11219226307088372Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA426520.11063591058215418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATACCGGCACGAGACCGATAGTCAACAGATCGGAAGAGCACACGTCTG421280.10927669607533037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA405410.10516014374264072No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT403810.10474511641231285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC393000.10194108801178511Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTGAA392620.10184251902083225Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)