FastQCFastQC Report
Fri 14 Jul 2023
SRR8891627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8891627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85335062
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length63
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCTGTAGGCACCATCAA7695050.9017454044856732No Hit
TCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCAAT6212580.7280219706174234No Hit
TCGCCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCTGTAGGCACCATCAA5691240.6669286769839108No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCAA3189980.3738182085108229No Hit
TAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCTGTAGGCACCATCA2827280.33131516327954386No Hit
TAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCCTGTAGGCACCATCAAT1508210.17673977901369545No Hit
TCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCA1469720.17222932351065734No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC1359360.1592967729958408No Hit
TGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCAA1243480.1457173605850313No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTGTAGGCACCATCAAT1110810.13017040990724305No Hit
CAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCTGTAGGCACCATCAA1088040.12750210458627192No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA940290.11018800220711153No Hit
TCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCTGTAGGCACCATCAA920010.10781148785009378No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)