FastQCFastQC Report
Thu 28 Sept 2023
SRR8899089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78940064
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT39239504.970796578021522No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG27574893.493142594867924No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA16666192.111246071449853No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG15623741.9791901866205732No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC12721181.6114985668113975RNA PCR Primer, Index 1 (100% over 50bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA10615251.3447227506681525No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG9320271.1806767726968146No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA8371461.060483052053264No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGG8269661.0475871922272573Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA8056521.0205869607605083No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA7808430.989159319658013RNA PCR Primer, Index 1 (100% over 23bp)
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCTGGAATTCTCGGGTGCC6295220.7974683172286254No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA6251160.791886867484678No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA5841810.74003106964798RNA PCR Primer, Index 1 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC5343200.6768679589618777No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCTGGAATTCTCGGGTGCCAAG4783980.6060268712221971No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG4073910.5160763487599909No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG3954420.500939548262844Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA3790550.4801807609378173RNA PCR Primer, Index 1 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC3443670.4362385619550549No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACTGGAATTCTCGG3351220.42452714505019906No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA3081300.3903341147531879RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCA2908080.3683908845070103No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAA2569720.325527985383949No Hit
GGAAGGACGGACGGCGCCGGACGCGCTGGAATTCTCGGGTGCCAAGGAAC2468430.3126967315354596RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2387520.3024471832199173No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCTGGAATTCTCGGGTGCCAA2335540.295862440648642No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2279740.28879378663792316Illumina Small RNA Adapter 2 (100% over 21bp)
TACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAG2106480.26684548925625395No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1996480.2529108666544785No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCTGGAATTCTCGGG1949820.24700005310357995No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1931470.24467550469682922RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1902880.24105376960424052No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCTGGAATTCTCGGGTGCCAAG1885000.23878876003951555No Hit
GGGGGGCGGGAAAGATCCGCCGGGCCGCCGACTGGAATTCTCGGGTGCCA1814810.22989720403570996No Hit
GGACGGCGCCGGACGCGCACGCCCCGCCGGGCCTGGAATTCTCGGGTGCC1767530.22390784988469228No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAA1751210.22184045860413792RNA PCR Primer, Index 1 (100% over 23bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCTGGAATTCTCGGGTGCC1603530.20313259436931796No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGT1567680.19859117418501207No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACTGGAATTCTCGGGTGCC1544450.19564843524829167No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC1499830.1899960456074624No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGG1476520.18704317239975887Illumina Small RNA Adapter 2 (100% over 21bp)
AAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCTGGAATTCTCGGGTGCCA1442180.18269303658025915No Hit
AAAAGCGACGTCGCTATGAACGCTTGGCCGCCTGGAATTCTCGGGTGCCA1435000.1817834857595251No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA1414690.1792106477136882RNA PCR Primer, Index 1 (100% over 22bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT1369320.1734632492823923No Hit
AGAACTAGTACCGCAAGGGAAAGATGAAAGACTTGGAATTCTCGGGTGCC1323410.16764744452196037No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG1280140.16216607070397104No Hit
GGGAGAGCCTCGATCAGAAGGACTTGGGCCCTGGAATTCTCGGGTGCCAA1259880.1595995665774986No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACTGGAATTCTCGGGTGCCAAGG1258850.15946908783859107Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCTGGAATTCTCGGGTG1199110.1519013209819541No Hit
AGACGAACGGAAGGACGGACGGCGCCGGACGCGCTGGAATTCTCGGGTGC1188870.1506041342961161No Hit
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT1151710.14589676542446178RNA PCR Primer, Index 1 (100% over 25bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA1141530.1446071794418611No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1119250.14178478497306513RNA PCR Primer, Index 1 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA1105610.14005689177044497No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA1098400.13914354059809225RNA PCR Primer, Index 1 (100% over 23bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1053330.13343414568298298RNA PCR Primer, Index 1 (100% over 30bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGCCA1024530.1297858081290636No Hit
AATGCCTAAAGGAAAGATCCAAAAAGTGGAATTCTCGGGTGCCAAGGAAC990610.12548887723222518RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC956720.12119574668700549No Hit
GCGGCGCTCCCCCGGGGAGGGGGGAGGACTGGAATTCTCGGGTGCCAAGG953570.12079670976704554Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG949230.12024692556621185No Hit
CACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGG911290.11544074755247222No Hit
GGGAGAAAGCCTACAGCACCCGGTATTGGAATTCTCGGGTGCCAAGGAAC905780.11474274963851054RNA PCR Primer, Index 1 (100% over 24bp)
AAAGCGACGTCGCTATGAACGCTTGGCCGCCTGGAATTCTCGGGTGCCAA900760.11410682413432044No Hit
GGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTGGAATTCTCGGGTGCCA895100.11338982446226545No Hit
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC880590.11155172106270397RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCTGGAATTCTCGGGTGC879910.11146557975934754No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGTGGAATTCTCGG879380.11139844021408445No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCTGGAATTCTCGGGTGCCAAGGA876740.11106400927164183RNA PCR Primer, Index 1 (100% over 22bp)
GGGAGAGCCTCGATCAGAAGGACTTGGGCCCCTGGAATTCTCGGGTGCCA869340.11012658920570421No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGG860210.10897001552975685Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACTGGAATTCTCGGGTG860170.10896494839426529No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA840770.10650738768086127No Hit
CTACTGGCAGGATCAACCAGGTAGGTAGGCTGGAATTCTCGGGTGCCAAG835300.10581445690239115No Hit
GGGAGAGCCTCGATCAGAAGGACTTGGGCCCCCCTGGAATTCTCGGGTGC821930.10412076686433899No Hit
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC800450.10139971510537413No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA797210.10098927713055819No Hit
AATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGA793940.1005750388041236RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)