FastQCFastQC Report
Thu 28 Sept 2023
SRR8899090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84887109
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG48720695.7394686394609105No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG48157755.673152327522427No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC30773393.6252135763040294RNA PCR Primer, Index 4 (100% over 50bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGG22361472.634259814408334Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG20933562.4660469942497394Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG15194481.7899631851050553No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT15116731.780803961647463No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA13890461.6363450426848676No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA13108351.544209733894931RNA PCR Primer, Index 1 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG11617301.3685587996641517No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA10259541.2086098962328897No Hit
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT10009301.1791307441039134RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT7187380.846698643017752RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA5455780.6427100727390775No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGT4646140.5473316331222918No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT4076550.48023192779483154No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCTGGAATTCTCGGGTGCCAA4029900.47473639372027615No Hit
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG3738810.44044496791615323RNA PCR Primer, Index 1 (100% over 29bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGTGGAATTCTCGGGT3151340.37123893570223954No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAA3029840.3569258083697962No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAA2910290.3428423978957747RNA PCR Primer, Index 1 (100% over 23bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2878140.3390550148197414RNA PCR Primer, Index 1 (100% over 31bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGCCA2742130.32303255845360457No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2718790.32028302436356976No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGTGC2698580.31790221528218143No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCTGGAATTCTCGGGTGCC2542870.2995590296283974No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA2465140.29040216224114784No Hit
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC2393630.28197803273050565RNA PCR Primer, Index 1 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG2352430.27712452782436026Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA2304230.2714463982982387RNA PCR Primer, Index 1 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG2138000.2518639196441476No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAA2122340.2500191165657438No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCA2041460.24049116809950494No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1975990.23277857183238507No Hit
AAATCCACCATCCGAGCTGAAAGTCGATTGGAATTCTCGGGTGCCAAGGA1939430.2284716752457667RNA PCR Primer, Index 1 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCAA1937170.22820543929703155No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA1850360.21797891597415572RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA1743310.20536804946437742RNA PCR Primer, Index 1 (100% over 22bp)
GGACGGCGCCGGACGCGCACGCCCCGCCGGGCCTGGAATTCTCGGGTGCC1686660.19869448021842753No Hit
AGCTCGTGTCGTGAGATGTTGGGTTTGGAATTCTCGGGTGCCAAGGAACT1674690.19728437211826827RNA PCR Primer, Index 1 (100% over 25bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCTGGAATTCTCGGGTGCCAAG1660240.1955821112956032No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCTGGAATTCTCGGGTGCCAAG1569520.18489497622071216No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA1488800.1753858763172156No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC1444720.17019309728170856No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCA1376100.1621094199355994No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA1368250.16118466232605472RNA PCR Primer, Index 1 (100% over 22bp)
CCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGTGCCA1264960.14901673704072074No Hit
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT1212450.14283087435572814RNA PCR Primer, Index 1 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGC1204620.14190847281652624No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCTGGAATTCTCGGGTGCC1203440.1417694646663017No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGA1193360.140582005213536RNA PCR Primer, Index 1 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGATGGAATTCTCGGGTGCCAAGG1158610.13648833299294008Illumina Small RNA Adapter 2 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGATGGAATTCTCGGGTGCCA1154280.13597824376372625No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAA1148240.13526671052020398No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCC1114470.13128848574640467No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG1032640.12164862393888336No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAG1009150.11888141932127763No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC993810.11707431336835843No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACTGGAATTCTCGGGTGCC969330.11419048326878467No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGC965760.11376992471259682No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC950560.11197931125207716No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTG920160.10839808433103784No Hit
CCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGAAC915820.1078868170666526RNA PCR Primer, Index 1 (100% over 24bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA904690.10657566392089052RNA PCR Primer, Index 1 (100% over 23bp)
CGGAATGCCTAAAGGAAAGATCCAAAAAGATATGGAATTCTCGGGTGCCA876020.10319823708450243No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA855330.10076088231488717No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACTGGAATTCTCGG849950.10012709939267693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)