FastQCFastQC Report
Thu 28 Sept 2023
SRR8899091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43660252
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1134632825.987774875875658RNA PCR Primer, Index 1 (100% over 50bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG4832561.1068557277223228No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA3330160.7627441087605267No Hit
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3062980.7015488595897248RNA PCR Primer, Index 1 (100% over 30bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA3049820.6985346763458901No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG2631370.6026923527605842No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA2514690.5759678162187428RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG2171550.49737459142471285Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA2161370.4950429511950595No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2092810.47933988104328856No Hit
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT2002550.45866661511710927RNA PCR Primer, Index 1 (100% over 25bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA1969240.45103725008275264No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG1936970.44364608797951965No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA1735940.3976019194758656RNA PCR Primer, Index 1 (100% over 23bp)
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA1548660.35470706857120293No Hit
GGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1451820.3325267110231063RNA PCR Primer, Index 1 (98% over 50bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG1364820.3126001196694879No Hit
AATGCCTAAAGGAAAGATCCAAAAAGTGGAATTCTCGGGTGCCAAGGAAC1286240.29460205589285193RNA PCR Primer, Index 1 (100% over 24bp)
TAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCAA1034980.23705314389848234No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA936360.21446509287211626No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG899270.20596995179963687No Hit
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC882060.20202815137209928RNA PCR Primer, Index 1 (100% over 24bp)
TTTGAAGCGAACCCGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAA868080.19882615427872474RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA863890.19786647131583207RNA PCR Primer, Index 1 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAATGTGGAATTCTCGGGTGCCAA852610.1952828856782595No Hit
AATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGA849020.19446062748332282RNA PCR Primer, Index 1 (100% over 22bp)
TAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG737210.16885152197472428RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA711070.1628643829174417RNA PCR Primer, Index 1 (100% over 23bp)
TACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAAGGA657720.15064503063335502RNA PCR Primer, Index 1 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC638680.14628408466355164No Hit
AGTTCTACAGGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG584550.1338860801811222RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCAA578810.1325713832343432No Hit
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG575350.131778900405797Illumina Small RNA Adapter 2 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCGTGGAATTCTCGGGTGCCAAGGAA560000.12826311675892296RNA PCR Primer, Index 1 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAA555780.12729656255763253No Hit
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT551290.12626816721076187RNA PCR Primer, Index 1 (100% over 25bp)
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA550040.12598186561085356RNA PCR Primer, Index 1 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCA549950.12596125189566015No Hit
AGTTCTACAGTCCGACGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG544630.12474275228645038RNA PCR Primer, Index 1 (100% over 29bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC540150.12371664735237901RNA PCR Primer, Index 1 (100% over 31bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA524810.12020315411830423No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACCACGATCTCGTATGC500290.1145870619345028RNA PCR Primer, Index 1 (98% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG497970.11405568616507297Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGCTCTCGTATGC490810.11241575060079818RNA PCR Primer, Index 1 (98% over 50bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC484250.11091323980447935RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCACGATCTCGTATGC468760.1073653903784156RNA PCR Primer, Index 1 (98% over 50bp)
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCTGGAATTCTCGGGTGCCAA460000.10535898876625814No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG458940.1051162050095359No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAA456180.10448405107693837No Hit
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC455850.10440846745456256RNA PCR Primer, Index 1 (98% over 50bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG444690.10185236677058118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)