FastQCFastQC Report
Fri 29 Sept 2023
SRR8899092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243382145
Total Bases12.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC157813446.48418313512686RNA PCR Primer, Index 6 (100% over 50bp)
TTTGAAGCGAACCCGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAA65055092.6729606643905615RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG44289071.8197337360142012No Hit
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT38256011.5718494879729161RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT27102011.1135578577467136RNA PCR Primer, Index 1 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG25335991.0409962489236833No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG23454000.9636697055159901Illumina Small RNA Adapter 2 (100% over 21bp)
AGCTCGTGTCGTGAGATGTTGGGTTTGGAATTCTCGGGTGCCAAGGAACT20538390.8438741469716278RNA PCR Primer, Index 1 (100% over 25bp)
GCGGGCGTAGCTCAGTTGGTTAGAGTGCCGGCCTGTCTGGAATTCTCGGG19184850.7882603713596164No Hit
TAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG15662380.6435303625087205RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG12813820.5264897307894135No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA12781500.5251617779932048RNA PCR Primer, Index 1 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG11399270.46836919774866803No Hit
AGATACCCTGGTAGTCCACGCCGTAAACTGGAATTCTCGGGTGCCAAGGA9780710.40186637355833965RNA PCR Primer, Index 1 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA8814520.36216789855311693No Hit
GAAGGGGGCTAGCGTTGCTCGGAATCTGGAATTCTCGGGTGCCAAGGAAC8316500.34170542789817226RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA8147740.33477147635460275No Hit
AGTTCTACAGGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG7935160.3260370640582529RNA PCR Primer, Index 1 (100% over 29bp)
AGTTCTACAGTCCGACGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG7502930.3082777497913826RNA PCR Primer, Index 1 (100% over 29bp)
AGTACGAGTAAAGATGCTCGTTTCGCGCGGCAGGACTGGAATTCTCGGGT6562780.2696491971504319No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA6506110.26732076011574313RNA PCR Primer, Index 1 (100% over 23bp)
ATCTCAGTACCCGGAGGAAAGGACTGGAATTCTCGGGTGCCAAGGAACTC6504150.26724022832488387RNA PCR Primer, Index 1 (100% over 26bp)
AACCCTGACCGACAGCTAAGGCCCCCTGGAATTCTCGGGTGCCAAGGAAC6479450.26622536340946457RNA PCR Primer, Index 1 (100% over 24bp)
GGTTTGAAGCGAACCCGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGG6453890.26517516311642336Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA6320390.2596899620553513No Hit
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA5840300.23996419293617452RNA PCR Primer, Index 1 (100% over 28bp)
AACGGAGGCGTACGAAGGTGGGCTCTGGAATTCTCGGGTGCCAAGGAACT5781260.23753837817478354RNA PCR Primer, Index 1 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA5407250.22217118679761821No Hit
AACGCGCAGAACCTTACCATCCTTTGACTGGAATTCTCGGGTGCCAAGGA4990400.20504380056310212RNA PCR Primer, Index 1 (100% over 22bp)
ACGAAGGGGGCTAGCGTTGCTCGGAATCTGGAATTCTCGGGTGCCAAGGA4928300.20249225759761463RNA PCR Primer, Index 1 (100% over 22bp)
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA4881300.20056113812293008No Hit
AGAGCATTCGACTGTTAATCGAAGGGTCTGGAATTCTCGGGTGCCAAGGA4733220.19447687914822182RNA PCR Primer, Index 1 (100% over 22bp)
AGCGAACCAGTACCGTGAGGGAAAGGTGAAAAGCTGGAATTCTCGGGTGC4141530.1701657284678792No Hit
AGGTGGAGGCGCAGCGAAAGCGAGTCTGAACTGGAATTCTCGGGTGCCAA4111680.1689392621632125No Hit
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC3974420.16329957154416566RNA PCR Primer, Index 1 (100% over 24bp)
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA3808680.15648970469875678No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC3777400.15520448305688161No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA3706960.1523102691037586RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA3617460.14863292457217844No Hit
ATCTCAGTACCCGGAGGAAAGGACATCTGGAATTCTCGGGTGCCAAGGAA3528040.1449588670524701RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACGGACGGGATAACCGCTGAAGGCTGGAATTCTCGGGTGCCAAGGA3491040.14343862406176097RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT3401170.13974607710027373RNA PCR Primer, Index 1 (100% over 25bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3393880.13944654814345564RNA PCR Primer, Index 1 (100% over 31bp)
AAGACACGGGAGAGTCGGTCGCCGCCAGACCCTGGAATTCTCGGGTGCCA3378380.1388096895932937No Hit
AAGCCCCGGCTAACTTCGTGCCAGCTGGAATTCTCGGGTGCCAAGGAACT3204250.13165509737782943RNA PCR Primer, Index 1 (100% over 25bp)
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG3120760.1282246896131185No Hit
TTCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACT3088710.1269078304819772Illumina DpnII expression Sequencing Primer (100% over 25bp)
GTGGAATCTGCTGTGAATCTGCCGGGACCTGGAATTCTCGGGTGCCAAGG3022460.12418577377563994Illumina Small RNA Adapter 2 (100% over 21bp)
AACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGC2961680.12168846650603725No Hit
TACGAGTAAAGATGCTCGTTACGCGCGGCAGGACTGGAATTCTCGGGTGC2845450.11691284913279074No Hit
ACGTCGAGCAGAGACGAAAGTCGGTCTGGAATTCTCGGGTGCCAAGGAAC2821130.11591359752376247RNA PCR Primer, Index 1 (100% over 24bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC2786530.11449196488920747RNA PCR Primer, Index 1 (100% over 27bp)
AGTCCACGCCGTAAACGATGAATGCCTGGAATTCTCGGGTGCCAAGGAAC2644770.10866737985237168RNA PCR Primer, Index 1 (100% over 24bp)
AATGCCTAAAGGAAAGATCCAAAAAGTGGAATTCTCGGGTGCCAAGGAAC2471010.10152799006681447RNA PCR Primer, Index 1 (100% over 24bp)
ACGTGAGAGTACTGCTCGGGAGACTGGAATTCTCGGGTGCCAAGGAACTC2468600.10142896883417639RNA PCR Primer, Index 1 (100% over 26bp)
TCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTC2453570.1008114214787613RNA PCR Primer, Index 1 (100% over 26bp)
AGTGGGGAATATTGGACAATGGGCGCTGGAATTCTCGGGTGCCAAGGAAC2448870.10061830953129285RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)