FastQCFastQC Report
Thu 28 Sept 2023
SRR8899093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81687139
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG63251447.743133224435734No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG36513394.469906823398479No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG34180994.184378400129793No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA28238323.4568868913379376No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG27488573.3651037772298524Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC19387882.373431146854097RNA PCR Primer, Index 5 (100% over 50bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG18756662.296158272846353No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA17072482.089983834542179No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC14509481.7762257532363817No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG12553391.5367645572701476No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA12529841.5338816065035648No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA12043751.474375299152049No Hit
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT11649521.4261143360645794RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT11159011.3660669398643035RNA PCR Primer, Index 1 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA10768621.3182760630164805RNA PCR Primer, Index 1 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC9440751.1557204861832657No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC8888481.08811253629534No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA7061040.8644004535401835No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC6206320.7597670913655086RNA PCR Primer, Index 1 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA5646150.6911920369741434No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA4694590.5747036874433809RNA PCR Primer, Index 1 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGT4615100.5649726574461128No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA4185720.5124086914097946No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT4141460.5069904578247011No Hit
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG4087780.5004190439329745RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG4006490.49046766101087225Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3894200.47672131104995613No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC3711410.454344471533028No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGG3542400.4336545560739984Illumina Small RNA Adapter 2 (100% over 21bp)
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCTGGAATTCTCGGGTGCCAA3003700.36770782240274075No Hit
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2998190.3670332976161645RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT2820470.3452771188375198No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2700150.3305477499952593No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA2608560.31933545866993823RNA PCR Primer, Index 1 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC2456820.30075970710640265RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA2379340.29127473787519964No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG2365970.28963800531684675No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGA2353250.28808084464801736RNA PCR Primer, Index 1 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAA2292830.28068433147107774No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA2155490.26387140330621695RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCA2044610.250297663136421No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAA1908090.23358511797065143No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC1724080.21105892813800226No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA1678930.20553174227340731No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGAA1662530.20352408229158325RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT1606720.1966919174388027RNA PCR Primer, Index 1 (100% over 25bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCC1582420.19371715295353897No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGTGGAATTCTCGG1549150.18964429639285077No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA1522460.18637695219072367No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG1479280.1810909303605308No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1422630.17415593414282757RNA PCR Primer, Index 1 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCAA1416430.1733969407350648No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC1354390.16580210992577424No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCA1305050.1597619914194816No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA1247020.15265805795940535RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGTGGAATTCTCGGGT1196200.14643676038158224No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGTGC1186230.1452162500145831No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA1183780.14491632520022524RNA PCR Primer, Index 1 (100% over 22bp)
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA1115190.13651965458112078RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC1112520.1361927977426165No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCA1109160.1357814722829257No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGCCA1073840.13145765822450947No Hit
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT1069640.130943501399896RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1042390.12760760295448711No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGGAATTCTCGGG1008750.12348945162591629No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG980200.11999440940146038Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCTGGAATTCTCGGGTGCC939520.1150144333002041No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAA916750.11222696880104957No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGATGGAATTCTCGGGTGCCA904060.11067348068096743No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC883040.10810024819206852No Hit
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC877660.10744163778339698No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC872180.10677078554556796RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCC851080.10418775959334309No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAG848250.10384131582818684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)