FastQCFastQC Report
Thu 28 Sept 2023
SRR8899094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28359495
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC682872124.079134695452087RNA PCR Primer, Index 2 (100% over 50bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT7520342.6517891097849238RNA PCR Primer, Index 1 (100% over 30bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG4603011.6230930769394871No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG4010441.4141436580587914No Hit
TAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG2543100.8967367014116436RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA2471710.8715634745964271RNA PCR Primer, Index 1 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA2389560.8425961040561548No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG1658280.5847353769874958Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA1444860.5094801582327189No Hit
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT1396720.49250524383456057RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1186320.4183149241550317No Hit
AGTTCTACAGGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG1137740.4011848588982279RNA PCR Primer, Index 1 (100% over 29bp)
AGTTCTACAGTCCGACGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG1090420.38449908928209053RNA PCR Primer, Index 1 (100% over 29bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1005310.35448797660184006RNA PCR Primer, Index 1 (100% over 31bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA999950.3525979570510688No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGT995550.35104644846461475No Hit
TTTGAAGCGAACCCGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAA994690.3507431990590806RNA PCR Primer, Index 1 (100% over 23bp)
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCTGGAATTCTCGGGTGCCAA966920.3409510641850287No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG898040.3166629024952666No Hit
GGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC845590.29816821491355894RNA PCR Primer, Index 2 (98% over 50bp)
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC824770.2908267583749288RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA794010.2799803028932638No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA757260.26702168004049437No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA736470.2596908019694991RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGTGC729500.25723307132232076No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG720260.25397490329076733No Hit
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA668030.2355577911383824RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGTATCTCGTATGC640340.22579386551135694RNA PCR Primer, Index 2 (98% over 50bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA600540.21175976511570463No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT585880.2065904205981101No Hit
AATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGA580580.20472155798260866RNA PCR Primer, Index 1 (100% over 22bp)
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA546830.1928207818933306No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGGGGAATTCTCGGGTGCCAA507550.17897004160335012No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCAA502550.17720696366419783No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAA491570.17333524450981938No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC488690.17231971161686765No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA451050.1590472608909291RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCA449910.1586452791208024No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATGTGGAATTCTCGGGTGCCAA425450.15002030184246934No Hit
AATGCCTAAAGGAAAGATCCAAAAAGTGGAATTCTCGGGTGCCAAGGAAC419100.14778119285974592RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA412100.1453128837449327No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTG403500.14228038968959075No Hit
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT399630.14091576736468686RNA PCR Primer, Index 1 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAA385920.13608140765553123No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT382110.1347379422658972No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC355800.12546062615007778No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA351610.12398316683706816RNA PCR Primer, Index 1 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGGGGAATTCTCGGGTGCCA340590.12009734305917649No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA334160.11783002482942662RNA PCR Primer, Index 1 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGTGGAATTCTCGGGTGCCAAGGAA318240.1122163846711657RNA PCR Primer, Index 1 (100% over 23bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC307440.1084081363225967RNA PCR Primer, Index 2 (98% over 50bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCTGGAATTCTCGGGTGCC301050.10615492271636008No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA299870.10573883632272014RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTCTCTCGTATGC294340.10378887212201769RNA PCR Primer, Index 2 (98% over 50bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGGAATTCTCGGG289340.10202579418286538No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCC286950.10118304292795058No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG286230.10092915970471267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)