FastQCFastQC Report
Fri 29 Sept 2023
SRR8899095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82527477
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG37456834.538710180125827No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC19774542.3961159021013088RNA PCR Primer, Index 3 (100% over 50bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA17073432.068817637548764RNA PCR Primer, Index 1 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGG14485931.7552856971502957Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA12286071.488724779505861No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG11491661.3924647181447216No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA9739801.1801887509538187No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA9661581.1707106955420437No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCTGGAATTCTCGGGTGCCAA8418711.020109944715746No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA8192910.9927493603130506No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA8018140.9715721710479529RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA7164390.8681217771870089RNA PCR Primer, Index 1 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG6652480.8060927392703402No Hit
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC5789560.7015312003298004RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG4527810.5486427265915327Illumina Small RNA Adapter 2 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT4465780.5411264420454778RNA PCR Primer, Index 1 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGT3930550.4762716785828797No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT3800700.46053752497486383No Hit
GGGAGAGCGACGCTCAGACAGGTGGAATTCTCGGGTGCCAAGGAACTCCA3702900.44868692641603475RNA PCR Primer, Index 1 (100% over 28bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3351050.4060526411100633RNA PCR Primer, Index 1 (100% over 30bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC3176640.3849190736801514RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA3003360.36392243034401744RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAA2793390.33847999497185643RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCA2429200.29435045009312477No Hit
GGGAGAAAGCCTACAGCACCCGGTGGAATTCTCGGGTGCCAAGGAACTCC2297850.278434538838501RNA PCR Primer, Index 1 (100% over 27bp)
CTACTGGCAGGATCAACCAGGTAGGTAGGCTGGAATTCTCGGGTGCCAAG1887040.22865596630319862No Hit
GGGAGAGCCTCGATCAGAAGGACTTGGGCCCTGGAATTCTCGGGTGCCAA1713250.20759752536721798No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG1675500.2030232912609215No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGA1644030.19921001583508968RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGAAC1600920.19398630107158127RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCC1575340.19088672733809614RNA PCR Primer, Index 1 (100% over 27bp)
TACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAAGGA1571650.19043960352744094RNA PCR Primer, Index 1 (100% over 22bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGCCA1470040.17812734054622803No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAA1456730.17651454436199474No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1442080.17473937801345849No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT1406830.17046807331817498No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCTGGAATTCTCGGGTGCC1356110.16432224142754298No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAAGGAA1355650.16426650241591656RNA PCR Primer, Index 1 (100% over 23bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA1323420.1603611364491368RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT1292010.15655513132916932RNA PCR Primer, Index 1 (100% over 25bp)
GGGAGAAAGCCTACAGCACCCGGTATTGGAATTCTCGGGTGCCAAGGAAC1237380.14993551783971296RNA PCR Primer, Index 1 (100% over 24bp)
GGGAGAGCGACGCTCAGACAGGCGTAGCCCTGGAATTCTCGGGTGCCAAG1235550.1497137735108514No Hit
GGAAGGACGGACGGCGCCGGACGCGCTGGAATTCTCGGGTGCCAAGGAAC1226100.14856870033722222RNA PCR Primer, Index 1 (100% over 24bp)
AAAAGCGACGTCGCTATGAACGCTTGGCCGCCTGGAATTCTCGGGTGCCA1203700.14585445281454565No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA1173400.1421829483530679RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG1073390.13006456019490334Illumina Small RNA Adapter 2 (100% over 21bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCTGGAATTCTCGGGTGCCAAG1045490.1266838679679981No Hit
AATGCCTAAAGGAAAGATCCAAAAAGTGGAATTCTCGGGTGCCAAGGAAC1039340.1259386616169061RNA PCR Primer, Index 1 (100% over 24bp)
AAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTGGAATTCTCGGGTGCCAA982440.1190439882222499No Hit
TCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTC968480.11735243039115324RNA PCR Primer, Index 1 (100% over 26bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCTGGAATTCTCGGGTG909780.1102396478205677No Hit
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC905670.1097416318688623RNA PCR Primer, Index 1 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT891030.10796767723797009RNA PCR Primer, Index 1 (100% over 25bp)
AGACGAACGGAAGGACGGACGGCGCCTGGAATTCTCGGGTGCCAAGGAAC889010.10772291027387158RNA PCR Primer, Index 1 (100% over 24bp)
CCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGTGCCA857450.10389872938924329No Hit
AACCCAAAAGGTCAGAAGGATCGTGAGGTGGAATTCTCGGGTGCCAAGGA842640.1021041755584022RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA835620.10125354977227766No Hit
CCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGAAC835160.10119781076065126RNA PCR Primer, Index 1 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCAA826330.1001278640809533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)