FastQCFastQC Report
Thu 28 Sept 2023
SRR8899096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68279276
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG50812267.441827590556175No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC48833777.152063240975197RNA PCR Primer, Index 6 (100% over 50bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG31119554.557685995381673No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG15298002.2405041318832963Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG14922192.1854640052129435No Hit
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT14604042.138868607804219RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGG14316692.0967840959532142Illumina Small RNA Adapter 2 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA13593591.9908808054730984No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA12140311.7780373066638842RNA PCR Primer, Index 1 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT9708481.4218779941369033RNA PCR Primer, Index 1 (100% over 25bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG7368361.0791502827300044RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA7021511.0283515601424948No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT6845841.0026234021579257No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA6822560.9992138756714409No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGT5079940.7439944149378502No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG4955960.725836635994793No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCTGGAATTCTCGGGTGCCAA4635830.6789512530859291No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA4278730.6266513429345677RNA PCR Primer, Index 1 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC3435820.5032010005495665RNA PCR Primer, Index 1 (100% over 24bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC3361820.4923631586251735RNA PCR Primer, Index 1 (100% over 27bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAA3164140.46341147495471396No Hit
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT2984940.4371663226188866RNA PCR Primer, Index 1 (100% over 25bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2847070.416974251455156RNA PCR Primer, Index 1 (100% over 31bp)
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA2329510.34117379920665825RNA PCR Primer, Index 1 (100% over 23bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA2318390.33954519377153325RNA PCR Primer, Index 1 (100% over 28bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2309720.3382754087784996No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGTGC2104970.3082882718322907No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA2002060.29321634869121926No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA2000750.2930244895976929RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGGAATTCTCGGG1996310.29237421908222927No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCAA1922810.28160960581948763No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG1836680.268995236563434Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA1836150.2689176141820836No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCTGGAATTCTCGGGTGCC1696600.24847949471520467No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG1681780.24630899718385998No Hit
CCGTCAGCTCACATAGTGACGCAGGCAGTGCGACCTGGAATTCTCGGGTG1597540.2339714322688483No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGCCA1484580.21742761302858574No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGATGGAATTCTCGGGTGCCA1471440.21550316380038945No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1448280.21211121219270107RNA PCR Primer, Index 1 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGATGGAATTCTCGGGTGCCAAGG1425300.2087456229032071Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTCTACAGTCCGACGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG1330470.19485707493442078RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT1296900.18994050259115225RNA PCR Primer, Index 1 (100% over 25bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT1292370.18927705091659144No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGC1252820.1834846637799733No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGTGGAATTCTCGG1206370.17668172111256716No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGTGGAATTCTCGGGT1200390.17580590631921755No Hit
TCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTC1130990.16564176808201658RNA PCR Primer, Index 1 (100% over 26bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCA1094940.16036198157695755No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA1069780.15667711532266393RNA PCR Primer, Index 1 (100% over 22bp)
GTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT1069730.1566697924564988RNA PCR Primer, Index 6 (100% over 39bp)
AGTTCTACAGGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG1050100.15379483520006862RNA PCR Primer, Index 1 (100% over 29bp)
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCTGGAATTCTCGGGTGCC1039630.1522614270250903No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCTGGAATTCTCGGGTGCCAAG1024130.1499913385138999No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC1013670.1484593949121546No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCC997610.1461072902999147No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAG986680.1445065117562172No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAA949200.13901729127883547No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAA911310.13346802329889965No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACTGGAATTCTCGG897110.13138832930800262No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA887630.1299999138830939RNA PCR Primer, Index 1 (100% over 22bp)
GGACGGCGCCGGACGCGCACGCCCCGCCGGGCCTGGAATTCTCGGGTGCC859670.12590496712355298No Hit
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC857650.12560912333048171RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAATGGAATTCTCGGGTGCCAAG855710.12532499612327466No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTG837060.122593567043681No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA826880.12110263149246046No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGGAATTCTCGGGTG792750.11610404304814245No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGTGGAATTCTCGGGTGCCAAGGA791740.11595612115160682RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC790970.11584334901266383No Hit
TCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTC783360.11472880878233097RNA PCR Primer, Index 1 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC759550.11124165991449586No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCTGGAATTCTCGGGTGCCAAG747710.10950760520659299No Hit
CCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGAAC746610.10934650215096012RNA PCR Primer, Index 1 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTGGAATTCTCGGGTGCCA743700.10892031134014954No Hit
TAATCCACCTATAACTTCTCTGTTAACCCTGGAATTCTCGGGTGCCAAGG730160.10693727918263222Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCA726670.10642614312430612No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC712800.10439478005009895No Hit
TACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAG706520.10347502805975856No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGG702130.10283208041046012Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)