FastQCFastQC Report
Thu 28 Sept 2023
SRR8899097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8899097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80379748
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC1146215614.26000489576056RNA PCR Primer, Index 6 (100% over 50bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT14622401.8191646980530467RNA PCR Primer, Index 1 (100% over 30bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA8821961.0975351652010654No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG6855040.8528317356755087No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA6684940.831669688738014No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA6617140.8232347282303No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG6453880.8029236419103976No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA5977500.7436574695407107RNA PCR Primer, Index 1 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA5891280.732930887018954RNA PCR Primer, Index 1 (100% over 23bp)
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG5647980.7026620685598567No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCA5350200.6656154234273041No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGTGGAATTCTCGGGTGCCAAGGAA4366920.5432861023649888RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA3988520.4962095676139716RNA PCR Primer, Index 1 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT3841060.4778641505569289RNA PCR Primer, Index 1 (100% over 25bp)
TAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCAA3143780.3911159313413125No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG3133220.389802167580819Illumina Small RNA Adapter 2 (100% over 21bp)
TACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAAGGA2533540.3151963103940062RNA PCR Primer, Index 1 (100% over 22bp)
TAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG2514080.31277530255506647RNA PCR Primer, Index 1 (100% over 29bp)
AATGCCTAAAGGAAAGATCCAAAAAGTGGAATTCTCGGGTGCCAAGGAAC2473740.3077566254624237RNA PCR Primer, Index 1 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG2450220.3048305152685973No Hit
AATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGA2106580.26207845289587123RNA PCR Primer, Index 1 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAATGTGGAATTCTCGGGTGCCAA2046060.2545491931624369No Hit
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2006440.24962009087164594RNA PCR Primer, Index 1 (100% over 31bp)
AGTTCTACAGTCCGACGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG1940440.24140906736856155RNA PCR Primer, Index 1 (100% over 29bp)
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCTGGAATTCTCGGGTGCCAA1904660.23695769735431366No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA1885700.23459889423888217No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAAGGAA1835560.22836100456547834RNA PCR Primer, Index 1 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAA1790420.22274516212715673No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA1789800.2226680282700065RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGT1759880.2189456976152749No Hit
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG1719280.21389467406640789Illumina Small RNA Adapter 2 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC1680720.20909744578945433RNA PCR Primer, Index 1 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA1653680.20573341434213005RNA PCR Primer, Index 1 (100% over 23bp)
AGTTCTACAGGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG1650260.20530793403333386RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1616160.2010655718900736No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG1604540.19961993411574266No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA1537280.19125215470941762No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA1414440.17596969823791933RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1355460.1686320290528903No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAA1324500.16478031257326162No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCA1033880.12862443908134671No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG1001680.12461845488741766Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT955900.1189229904030055No Hit
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT955400.11886078567949727RNA PCR Primer, Index 1 (100% over 25bp)
TAATCCACCTATAACTTCTCTGTTAACCCAACTGGAATTCTCGGGTGCCA897480.11165499050830566No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC837580.1042028646320215No Hit
AATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCAAG835540.10394906936010796No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCCATATCTCGTATGC823640.10246859694061246RNA PCR Primer, Index 6 (98% over 50bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACCTGGAATTCTCGGGTGCCAA816800.10161763632302007No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCAAGGA803900.10001275445650812RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)