FastQCFastQC Report
Fri 14 Jul 2023
SRR8907185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8907185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95271970
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG5882127461.74037757380266TruSeq Adapter, Index 7 (100% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG77476828.1321736078303TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC11939071.253156620987264TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATCATCTCGTATG7018860.7367182603655619TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATCATCTCGTATG6964540.7310166883292116TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATAATCTCGTATG5925980.6220066615605828TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATCATCTCGTATG5491460.576398283776435TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAACATCTCGTATG5105770.5359152329903538TruSeq Adapter, Index 7 (97% over 49bp)
GGGAGAGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT3842560.4033253432252949TruSeq Adapter, Index 7 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCTCATCTCGTATG3718880.39034356065063TruSeq Adapter, Index 7 (97% over 49bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC3313180.34776020691080495TruSeq Adapter, Index 7 (100% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATAATCTCGTATG3231570.33919420370965353TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTTCATCTCGTATG2806550.29458297125586885TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCATCTCGTATG2346900.2463368816662445TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATGATCTCGTATG2204090.23134716328422728TruSeq Adapter, Index 7 (97% over 49bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA2037860.21389921925619887No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACATCATCTCGTATG1688840.17726514944531954TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAGCATCTCGTATG1678430.17617248808857422TruSeq Adapter, Index 7 (97% over 49bp)
ATCCCGGACGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG1477350.1550665951381083TruSeq Adapter, Index 7 (100% over 36bp)
GGCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1463960.153661145035628Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC1339240.1405702012879549TruSeq Adapter, Index 7 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGATCATCTCGTATG1309120.13740872577737187TruSeq Adapter, Index 7 (97% over 49bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1302000.13666139159293128TruSeq Adapter, Index 7 (100% over 35bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT1290480.13545222167653298TruSeq Adapter, Index 7 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAACATCTCGTATG1267220.13301079005713853TruSeq Adapter, Index 19 (97% over 38bp)
GGGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT1259320.1321815849929418TruSeq Adapter, Index 7 (100% over 41bp)
GGGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA1224630.1285404300971209TruSeq Adapter, Index 7 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATAATCTCGTATG1020040.1070661181877524TruSeq Adapter, Index 7 (97% over 38bp)
GCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1006190.10561238525874923Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)