FastQCFastQC Report
Mon 17 Jul 2023
SRR8907186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8907186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59185502
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4335539573.25340418672127TruSeq Adapter, Index 8 (100% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG56333599.518140101270072TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAGAATCTCGTATG19437653.284191118291098TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATGAATCTCGTATG6325471.068753290290585TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3419930.5778323887495286TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATTGAATCTCGTATG2992100.5055461048552059TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGCATCTCGTATG2981600.5037720217359988TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTGAATCTCGTATG2646670.44718214943923257TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTGAATCTCGTATG2014040.3402927967055175TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATAGAATCTCGTATG1587870.2682869869043267TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGAATCTCGTATG1361850.23009858056116514TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTTAATCTCGTATG1304920.22047967084912112TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGGAATCTCGTATG1237660.20911540126837144TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATGCATCTCGTATG801640.13544533254106725TruSeq Adapter, Index 8 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGAATCTCGTATG798340.134887763560745TruSeq Adapter, Index 2 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAGCATCTCGTATG776900.1312652547916211TruSeq Adapter, Index 8 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGAGAATCTCGTATG760440.12848416830189258TruSeq Adapter, Index 15 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATAATCTCGTATG692900.11707258983796402TruSeq Adapter, Index 8 (97% over 36bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAGAATCTCGTATG616080.10409305981725053TruSeq Adapter, Index 8 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)