FastQCFastQC Report
Fri 14 Jul 2023
SRR8907187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8907187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64728958
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG3646846256.340258095920525TruSeq Adapter, Index 9 (100% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG57569078.893866327957882TruSeq Adapter, Index 9 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAAGATCTCGTATG8374891.2938397679752547TruSeq Adapter, Index 9 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC7767071.1999374375839635TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATG6658371.028653975860387TruSeq Adapter, Index 9 (97% over 49bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC4632340.7156518725359367TruSeq Adapter, Index 9 (100% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG4173860.6448211324520318TruSeq Adapter, Index 9 (97% over 49bp)
GGGAGAGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA4145300.6404088877809527TruSeq Adapter, Index 9 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGATCTCGTATG2997040.4630137874303492TruSeq Adapter, Index 9 (97% over 49bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT2004970.30974853635060834TruSeq Adapter, Index 9 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCATATCTCGTATG1881910.29073695269434124TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAGATCTCGTATG1830170.2827436214870012TruSeq Adapter, Index 9 (97% over 49bp)
GGGAGAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG1673790.25858441904780854TruSeq Adapter, Index 9 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGATCTCGTATG1559740.24096479353182235TruSeq Adapter, Index 10 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAAAGATCTCGTATG1502320.23209395708177474TruSeq Adapter, Index 7 (97% over 35bp)
GGGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA1414640.21854824234927434TruSeq Adapter, Index 9 (100% over 40bp)
GGGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT1351850.20884779266800496TruSeq Adapter, Index 9 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCACATCTCGTATG1308220.20210737827727737TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAAGATCTCGTATG1192320.18420194559597267TruSeq Adapter, Index 19 (97% over 38bp)
GGGAGAGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA992430.15332086760920824TruSeq Adapter, Index 9 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCAGATCTCGTATG931180.1438583330817715TruSeq Adapter, Index 9 (97% over 49bp)
GGGAGAGCGACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA882740.13637482006121587TruSeq Adapter, Index 9 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAAGATCTCGTATG853580.13186988117435786TruSeq Adapter, Index 6 (97% over 38bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC811420.12535656761228875Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA809600.12507539515775923TruSeq Adapter, Index 9 (100% over 35bp)
ATCCCGGACGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT792980.1225077653806817TruSeq Adapter, Index 9 (100% over 36bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT779330.1203989719717101TruSeq Adapter, Index 9 (100% over 48bp)
GGCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC764390.11809088599881369Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA759540.11734160775460035TruSeq Adapter, Index 9 (100% over 35bp)
GGGAGAGCGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC737770.11397835262541998TruSeq Adapter, Index 9 (100% over 37bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT690860.10673120985510072TruSeq Adapter, Index 9 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGATCTCGTATG687240.10617195475323424TruSeq Adapter, Index 9 (97% over 49bp)
GGGAGAGCGACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG686910.10612097293455582Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)