FastQCFastQC Report
Tue 18 Jul 2023
SRR8907188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8907188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74345267
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG2976577340.037213128846524TruSeq Adapter, Index 10 (100% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG41442005.574262044145998TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTTATCTCGTATG11669241.5696009269830182TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATTATCTCGTATG7601521.0224618602822424TruSeq Adapter, Index 10 (97% over 49bp)
GGGAGAGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT5977730.8040498395143298TruSeq Adapter, Index 10 (100% over 38bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC5763430.775224870737232TruSeq Adapter, Index 10 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC5692100.7656304469254244TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG4993100.6716096668265379TruSeq Adapter, Index 10 (97% over 38bp)
ATCCCGGACGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG3712570.49936870897242186TruSeq Adapter, Index 10 (100% over 36bp)
GCCCACCCAGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3221880.43336719740343393Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA3100350.41702049439139144TruSeq Adapter, Index 10 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCTTATCTCGTATG3009990.4048663918309689TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCATATCTCGTATG2840820.3821117489563929TruSeq Adapter, Index 10 (97% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC2687390.3614742549784642Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTGATCTCGTATG2645960.35590160702496365TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTAATCTCGTATG2601790.3499604083740798TruSeq Adapter, Index 10 (97% over 49bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT2497320.3359084042296869TruSeq Adapter, Index 10 (100% over 41bp)
ATCCCAGCGGTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA2124130.2857115302309695TruSeq Adapter, Index 10 (100% over 35bp)
GGGAGAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT2083380.28023034741404584TruSeq Adapter, Index 10 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATAATCTCGTATG2006430.269879991149941TruSeq Adapter, Index 7 (97% over 38bp)
GGCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2004810.2696620889128019Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGATCTCGTATG1903290.2560068820520881TruSeq Adapter, Index 10 (97% over 38bp)
GCCCACCCAGGGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1874600.2521478603338663Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT1797330.24175446165254877TruSeq Adapter, Index 10 (100% over 41bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1658050.223020249560742Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG1642560.22093672755254212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCACCTGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA1613380.21701179713296345TruSeq Adapter, Index 10 (100% over 35bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT1613170.2169835505466676TruSeq Adapter, Index 10 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTTATCTCGTATG1593150.21429070931980107TruSeq Adapter, Index 10 (97% over 49bp)
GGGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA1545970.20794464293201073TruSeq Adapter, Index 10 (100% over 40bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1514400.20369823945887502Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC1405320.18902615549151233TruSeq Adapter, Index 10 (100% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATAATCTCGTATG1346270.18108348444024017TruSeq Adapter, Index 7 (97% over 38bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC1297400.17451010028654548No Hit
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG1295520.17425722608542116TruSeq Adapter, Index 10 (100% over 36bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACACG1269070.17069950128768788No Hit
TTCCCGGACGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG1190260.16009896097353446TruSeq Adapter, Index 10 (100% over 36bp)
GCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1160190.1560543188310831Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGAGCGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC1152350.1549997796093731TruSeq Adapter, Index 10 (100% over 37bp)
ATCCCGGACGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA1137350.15298216630253006TruSeq Adapter, Index 10 (100% over 35bp)
GGGAGAGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT1129680.1519504933649643TruSeq Adapter, Index 10 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTGATCTCGTATG1096860.1475359554495917TruSeq Adapter, Index 10 (97% over 38bp)
ATCACGTTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT1036280.13938748784102153TruSeq Adapter, Index 10 (100% over 38bp)
TTCCCGGGCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA999420.13442953940833918TruSeq Adapter, Index 10 (100% over 35bp)
CTGTCCCCGCCGGCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC989650.13311540060781543Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC981450.13201243866674123Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCCCGGCGTGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA963370.12958054209422637TruSeq Adapter, Index 10 (100% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT958610.1289402861381882No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC957630.12880846873547444Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATTATCTCGTATG953620.1282690934447784TruSeq Adapter, Index 7 (97% over 38bp)
GGGAGAGCGACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA947010.12737999851422957TruSeq Adapter, Index 10 (100% over 35bp)
GCCCCACGTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG936060.12590714080023413TruSeq Adapter, Index 10 (100% over 36bp)
TAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC910080.12241263455278195TruSeq Adapter, Index 10 (100% over 37bp)
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA878610.11817968183502522Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCGAGGGGGGCCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT875620.11777750424919452Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCGCGAGGGGGGCCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAG858150.11542765728449128Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA789920.10625020688943118TruSeq Adapter, Index 10 (100% over 35bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA788390.10604441033213317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAGCGACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT785310.10563012706646141Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC775570.1043200234925513TruSeq Adapter, Index 10 (100% over 44bp)
GGCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT772780.10394474741747851Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT760280.10226340299510929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGGGCGCGCCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC753660.10137296298902256Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCTCACACGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA747890.10059685440365694TruSeq Adapter, Index 10 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)