FastQCFastQC Report
Fri 14 Jul 2023
SRR8907189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8907189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53731143
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG3449468964.19868827283275TruSeq Adapter, Index 11 (100% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG685105912.750629555749446TruSeq Adapter, Index 11 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATACATCTCGTATG14105842.62526334122466TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAACATCTCGTATG9745921.8138307610541617TruSeq Adapter, Index 11 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC8336441.5515098943642425TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAACATCTCGTATG2713990.5051055772254835TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTACATCTCGTATG2527640.4704236423930159TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTACATCTCGTATG2427470.45178082290190624TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTCATCTCGTATG1924070.35809214034400866TruSeq Adapter, Index 11 (97% over 49bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC1596660.2971572743204067TruSeq Adapter, Index 11 (100% over 42bp)
GGGAGAGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA1415640.26346731540775153TruSeq Adapter, Index 11 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGGTACATCTCGTATG1062880.19781451513138293TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCATACATCTCGTATG858500.15977698445759847TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAACATCTCGTATG811650.1510576464007103TruSeq Adapter, Index 6 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTACATCTCGTATG773500.14395748104595504TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAAATCTCGTATG712360.13257860529786236TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATAAATCTCGTATG696870.1296957334408464TruSeq Adapter, Index 11 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGATCTCGTATG665850.12392254525462078TruSeq Adapter, Index 6 (97% over 38bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT619110.11522367949626533TruSeq Adapter, Index 11 (100% over 41bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATACATCTCGTATG602650.11216027918855179TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTACATCTCGTATG588590.10954354721246112TruSeq Adapter, Index 11 (97% over 49bp)
GGGAGAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC578510.10766754021964506TruSeq Adapter, Index 11 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAACATCTCGTATG572610.10656948057107217TruSeq Adapter, Index 6 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACGGCTACATCTCGTATGC558840.10400672101838593Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAAGATCTCGTATG542050.10088190381507425TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)