FastQCFastQC Report
Mon 17 Jul 2023
SRR8907190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8907190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63112705
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2000587031.698641343292135TruSeq Adapter, Index 12 (100% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG29477334.670585740224571TruSeq Adapter, Index 12 (100% over 49bp)
GGGAGAGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT6836951.0832921834042766TruSeq Adapter, Index 12 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTAATCTCGTATG6097160.9660748972809833TruSeq Adapter, Index 12 (97% over 49bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC6032760.9558709296329478TruSeq Adapter, Index 12 (100% over 42bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG5260320.8334803586694629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4462970.7071428803439815TruSeq Adapter, Index 12 (100% over 50bp)
GGGAGAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA4323320.6850157983246005TruSeq Adapter, Index 12 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGTAATCTCGTATG3459390.5481289385393955TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTAATCTCGTATG2866580.4542001487656091TruSeq Adapter, Index 12 (97% over 49bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT2644270.41897586230854783TruSeq Adapter, Index 12 (100% over 41bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT2334050.3698225262251079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAACT2291100.36301724034804084Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT2050350.3248711966948652TruSeq Adapter, Index 12 (100% over 35bp)
ATCCCGGACGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT1982550.31412851025795835TruSeq Adapter, Index 12 (100% over 36bp)
GGGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA1844700.29228663230327395TruSeq Adapter, Index 12 (100% over 40bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT1802080.28553363383806796TruSeq Adapter, Index 12 (100% over 36bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1796540.28465583910561276Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT1742430.2760822880274265TruSeq Adapter, Index 12 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGTAATCTCGTATG1735180.27493354943350945TruSeq Adapter, Index 12 (97% over 49bp)
GCCCACCCAGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1486340.235505671956225Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTGCGCTCGTGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1433280.22709848991577844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1350270.21394582913218502Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA1326310.21014944613766753TruSeq Adapter, Index 12 (100% over 39bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACACG1287210.20395418006564606No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTATAATCTCGTATG1259470.1995588685352656TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTAATCTCGTATG1162680.18422281218971678TruSeq Adapter, Index 12 (97% over 49bp)
GGGAGAGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT1162650.1842180587886385TruSeq Adapter, Index 12 (100% over 38bp)
GGGAGAGCGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG1153410.1827540112565291TruSeq Adapter, Index 12 (100% over 37bp)
CTGTCCCCGCCGGCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1138940.18046128746977333Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAGAGCGACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1111960.1761863954333759TruSeq Adapter, Index 12 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAAATCTCGTATG1089910.17269264564084205TruSeq Adapter, Index 12 (97% over 49bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1084110.17177365476570844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1070560.16962670194535318TruSeq Adapter, Index 12 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCTAATCTCGTATG1068200.16925276772719536TruSeq Adapter, Index 12 (97% over 49bp)
GGGAGAAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT1060780.16807709319383474TruSeq Adapter, Index 12 (100% over 38bp)
CCACGAGGAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG1018620.16139698021182897TruSeq Adapter, Index 12 (100% over 37bp)
GCCTCACCTGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1012380.1604082727875473TruSeq Adapter, Index 12 (100% over 35bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT964770.15286462527632114TruSeq Adapter, Index 12 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTAATCTCGTATG959910.15209457430164022TruSeq Adapter, Index 12 (97% over 49bp)
AGGACGAAGGGCACTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT948590.15030095762810358Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCCAGCGGTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT947090.15006328757418969TruSeq Adapter, Index 12 (100% over 35bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC927090.1468943535220048No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC893810.14162124725916914No Hit
GGGAGAGCGACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC893140.14151508796842094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCCACGTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT842670.13351828288773235TruSeq Adapter, Index 12 (100% over 36bp)
TAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG816200.12932419866966563TruSeq Adapter, Index 12 (100% over 37bp)
TTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC813090.1288314294245509TruSeq Adapter, Index 12 (100% over 44bp)
ATCACGTTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT797400.12634540066061184TruSeq Adapter, Index 12 (100% over 38bp)
GGGGAGAGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG791540.12541690298332167TruSeq Adapter, Index 12 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATAATCTCGTATG779510.12351078915093246TruSeq Adapter, Index 7 (97% over 38bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT775340.1228500664010519TruSeq Adapter, Index 12 (100% over 48bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC768960.12183917643840492TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTTTAATCTCGTATG764410.12111824394153284TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCTAATCTCGTATG760400.12048287266406978TruSeq Adapter, Index 9 (97% over 37bp)
GGGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT747620.11845792380472363TruSeq Adapter, Index 12 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTTATCTCGTATG721980.11439535034982258TruSeq Adapter, Index 12 (97% over 49bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT715160.11331474383802753TruSeq Adapter, Index 12 (100% over 43bp)
TTCCCGGACGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT659160.10444172849190983TruSeq Adapter, Index 12 (100% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG650840.1031234519262009TruSeq Adapter, Index 12 (100% over 50bp)
GGGAGAAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA650750.10310919172296608TruSeq Adapter, Index 12 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTAATCTCGTATG650050.1029982790311396TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)