FastQCFastQC Report
Fri 14 Jul 2023
SRR8919681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8919681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98790101
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length43
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT39288333.9769500792392143No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG38097183.8563762577791065No Hit
ACCCCGCCCCGAGTGTTACAGCCCCCCGGCTGGAATTCTCGGG21943982.2212731617715424No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGG15700321.5892604462465325No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG15691771.588394974917578No Hit
GCGACCCCGGGGGCCGGGCGGCGTCGGCTTGGAATTCTCGGGT14960071.5143288496081202No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG12640471.2795279964335697No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGT7677350.7771375798067056No Hit
CGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTG7389660.7480162410199378No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA5025010.5086552143518914Illumina Paired End PCR Primer 2 (100% over 22bp)
TCGGGTCTTCCCGGAGTCGGGTTGCTTGGGTGGAATTCTCGGG4476700.4531526898631271No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG4457380.4511970283338409No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGG4347120.4400359910554196No Hit
TTGGTAATTCTAGAGCTAATACATGCCGACTGGAATTCTCGGG4300130.4352794416112602No Hit
GCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT4195890.42472777712819626No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCG4025300.40745985268301327No Hit
GCGACCCCGGGGGCCGGGCGGCGTCGGCTTTGGAATTCTCGGG3877780.39252718245525425No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGG3674830.3719836261732337No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGT3610830.365505244295681No Hit
CCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT3590130.36340989265716006No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGTGGAATTCTCGG3288700.33289772626105524No Hit
TACCCCGCCCCGAGTGTTACAGCCCCCCGGCTGGAATTCTCGG3188390.32274387491515977No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGGT2991850.3028491690680628No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCG2968280.30046330249222036No Hit
TACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGG2930480.29663700819579075No Hit
TCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGGTG2891950.2927368198560704No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGG2865720.29008169553344215No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGT2814410.28488785531254796No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGGTGGAATTCTCGGG2761730.27955533722958736No Hit
TACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTCGG2741020.27745897334389813No Hit
TGGTAATTCTAGAGCTAATACATGCCGACTGGAATTCTCGGGT2733430.27669067774310707No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCG2470300.2500554180018502No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGG2359990.2388893194875871No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG2325420.2353899810265403No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGG2292050.2320121122256976No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGTGGAATTCTCGGGT2172840.21994511373158734No Hit
TACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCG2032340.20572304101602246No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGGCTGGAATTCTCGG2024960.20497600260576715No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG1987610.20119525943191413No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCG1942270.1966057307705354No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTTGGAATTCTCGG1885380.1908470566296921No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTTGGAATTCTCGGGTG1827770.1850155006927263No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGG1792650.18146048863741923No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGTGGAATTCTCGGG1719070.1740123739725704No Hit
TCTAGATAACCTCGGGCCGATCGCACGCCCTTGGAATTCTCGG1687460.17081266067336037No Hit
TCGGGGGGGCGTCGTCACGCGCTCTCCCTCCCCTTGGAATTCT1664710.16850979836532406No Hit
ACGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT1628260.16482015743662415No Hit
TCGACAACCCGCAGTTTTATCCGGTAAAGCGTGGAATTCTCGG1573080.1592345775615717No Hit
CCGGGGGGGCGTCGTCACGCGCTCTCCCTCCTGGAATTCTCGG1561580.15807049331794892No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGTGGAATTCTCGGG1550200.1569185560403466No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGG1502330.15207292884537085No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG1475260.14933277576059975No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGG1465110.14830534488470662No Hit
TCGCCGCGCTCTCCCCCCGGCCTCTCCCCTTGGAATTCTCGGG1382770.13997050170036773No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGT1356980.13735991625314767No Hit
GGGGGGGGCGTCGTCACGCGCTCTCCCTCCCCTTGGAATTCTC1355050.13716455254965274No Hit
ACCCCGCCCCGAGTGTTACAGCCCCCCGGTGGAATTCTCGGGT1313230.13293133489153938No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGG1301980.13179255682712585No Hit
GGGGGGGGGCGTCGTCACGCGCTCTCCCTCCCCTTGGAATTCT1286430.13021851248031419No Hit
AGTGCCGTTCCGAAGGGACGGGCGATGGCTGGAATTCTCGGGT1278780.12944414339651297No Hit
TTTGGTAATTCTAGAGCTAATACATGCCGACTGGAATTCTCGG1266690.12822033656995654No Hit
CGGGGGGGCGTCGTCACGCGCTCTCCCTCCCCTTGGAATTCTC1263810.12792880938546666No Hit
GCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGGTG1254810.12701778693393584No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGTGGAATTCTCGG1242820.12580410257906305No Hit
GGGGGGCCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCG1231030.12461066316755764No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGT1188270.12028229427561775No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGGT1152410.11665237592985152No Hit
TTGGGGGGGACGCGTGCATTTATCAGATTGGAATTCTCGGGTG1150960.11650560009043821No Hit
TCGCGACCCCGGGGGCCGGGCGGCGTCGGCTTGGAATTCTCGG1148920.11629910166809122No Hit
CGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGGT1148120.11621812189462181No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGG1137180.11511072349242764No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTGGAATTCTCGGG1099510.11129758840918687No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGG1088390.1101719695579621No Hit
TTGGGGGGGACGCGTGCATTTATCAGATCTGGAATTCTCGGGT1079680.10929030227431391No Hit
TGGCGCTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGG1057350.10702995434734905No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGTG1050670.10635377323887946No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTC1043580.10563608999650682No Hit
TGCGCTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGG1035330.10480098608260356No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGT1002530.10148081537035782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)