FastQCFastQC Report
Fri 14 Jul 2023
SRR8919682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8919682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101652889
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length43
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT32684213.2152760557547957No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG21867702.151212839607539No Hit
ACCCCGCCCCGAGTGTTACAGCCCCCCGGCTGGAATTCTCGGG10640041.046703158628379No Hit
GCGACCCCGGGGGCCGGGCGGCGTCGGCTTGGAATTCTCGGGT10346021.017779238915679No Hit
TCGGGTCTTCCCGGAGTCGGGTTGCTTGGGTGGAATTCTCGGG10072930.9909142867548013No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG9918860.9757578065489116No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA7142080.7025948864079996Illumina Paired End PCR Primer 2 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGG6498660.6392990955721878No Hit
TACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGG6458890.6353867621017637No Hit
TACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTCGG6252640.6150971272444603No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGT4859200.4780188785387103No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGGTGGAATTCTCGGG4774460.4696826668644902No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGG4447170.4374858445980812No Hit
ATACCGTCGTAGTTCCGACCATAAACGATGTGGAATTCTCGGG4415600.43438017782259No Hit
TACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCG4281470.4211852749212076No Hit
CGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTG3671900.3612194435516732No Hit
TTGGTAATTCTAGAGCTAATACATGCCGACTGGAATTCTCGGG3636540.3577409393647435No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTGGAATTCTCGGG3561130.35032255699097736No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGTGGAATTCTCGG3244250.3191498079311843No Hit
TTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCTGGAATTCTC3147720.3096537669480304No Hit
TACCCCGCCCCGAGTGTTACAGCCCCCCGGCTGGAATTCTCGG2975470.29270884765508237No Hit
GCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT2960730.2912588150839471No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGTGGAATTCTCGGGT2801520.2755966925839166No Hit
TCGCCGCGCTCTCCCCCCGGCCTCTCCCCTTGGAATTCTCGGG2779680.2734482047037542No Hit
TCGGGTCTTCCCGGAGTCGGGTTGCTTGGTGGAATTCTCGGGT2616880.25743291958972264No Hit
TATACCGTCGTAGTTCCGACCATAAACGATGTGGAATTCTCGG2588210.25461253737707346No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGGCTGGAATTCTCGG2465720.2425627076865469No Hit
GCGACCCCGGGGGCCGGGCGGCGTCGGCTTTGGAATTCTCGGG2418880.2379548701267113No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGTGGAATTCTCGGG2368640.23301256101044016No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGTGGAATTCTCGGG2350770.2312546178594098No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCG2236080.21997210526894126No Hit
TCGACAACCCGCAGTTTTATCCGGTAAAGCGTGGAATTCTCGG2195760.21600566610556438No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG2186750.21511931648101018No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGGT2139690.21048983664399348No Hit
TACCCGCCGGGCAGCTTCCGGGAAACCAAAGTGGAATTCTCGG2104030.2069818202609077No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGGT2077530.20437490960045415No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGG2065830.2032239339503671No Hit
AGTGCCGTTCCGAAGGGACGGGCGATGGCTGGAATTCTCGGGT2021660.1988787549363206No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGG2016720.19839278743961716No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGT2007400.19747594187903506No Hit
TCGTGGCGGCGACGACCCATTCGAACGTCTGTGGAATTCTCGG1944820.1913196977608772No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGTGGAATTCTCGG1861560.18312907958769375No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGTGGAATTCTCGGGT1821790.1792167461172697No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGG1815800.17862748593402003No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGT1800070.17708006311556967No Hit
CCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT1726850.16987711977374298No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGTGGAATTCTCGGGTG1707990.16802178637539755No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGT1704670.16769518473793696No Hit
AAAAGGGGGCCGCCCTCTCGCCCGTCACGCTGGAATTCTCGGG1577520.15518693226711933No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTTGGAATTCTCGGGTG1535690.1510719483830902No Hit
AGATACCGTCGTAGTTCCGACCATAAACGTGGAATTCTCGGGT1525770.15009607843019593No Hit
AAATACATGCCGACGGGCGCTGACCCCCCTTGGAATTCTCGGG1518650.149395655641425No Hit
TGGTAATTCTAGAGCTAATACATGCCGACTGGAATTCTCGGGT1511210.1486637531767543No Hit
TTACCCTACGCCGGCAGGCGCGGGTAACCCGTGGAATTCTCGG1502540.1478108507078436No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGG1473360.14494029776172915No Hit
ACGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT1458710.14349911884944067No Hit
ACCGGGGTCCGGTGCGGAGAGCCATTCGTGGAATTCTCGGGTG1439720.14163099683276095No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTTGGAATTCTCGG1430680.14074169598859113No Hit
ATGTCCGGGGCTGCACGCGCGCTACACTGTGGAATTCTCGGGT1411290.13883422437703663No Hit
TCAGAATCCCGCCCAGGCGGAACGATACGTGGAATTCTCGGGT1402890.13800788288466645No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGG1398570.13758290726001893No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGTG1394360.13716875277396198No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGT1379040.1356616632902583No Hit
ATTGAGCAATAACAGGTCTGTGATGCCCTTTGGAATTCTCGGG1368000.13457561447171462No Hit
AAAACTAACCAGGATTCCCTCAGTAACGGCGTGGAATTCTCGG1360050.13379354127357856No Hit
TCGCCGAAGGAGCCTCGGTTGGCCCCGGATTGGAATTCTCGGG1352750.133075411167114No Hit
ATTTCTGCCCAGTGCTCTGAATGTCAAAGTGGAATTCTCGGGT1330050.1308423216579708No Hit
TCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGGTG1312460.12911192322335277No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGG1303400.1282206548994392No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGTGGAATTCTCGGG1252670.12323014252944647No Hit
TCGAGTGCCGTTCCGAAGGGACGGGCGATGTGGAATTCTCGGG1248080.12277860592825847No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG1220780.1200929960780554No Hit
ATGAAGAACGCAGCTAGCTGCGAGAATTTGGAATTCTCGGGTG1181640.11624263821955912No Hit
AACGAGGAATTCCCAGTAAGTGCGGGTCATTGGAATTCTCGGG1175900.11567797153310616No Hit
TTGAAACTGCGAATGGCTCATTAAATCAGTTTGGAATTCTCGG1174060.11549696339668222No Hit
TCGCGCTACACTGACTGGCTCAGCGTGTGCCTGGAATTCTCGG1170190.1151162560662688No Hit
TCGCGACCCCGGGGGCCGGGCGGCGTCGGCTTGGAATTCTCGG1144900.11262837793031145No Hit
ATTGATCATCGACACTTCGAACGCACTTGTGGAATTCTCGGGT1133810.11153741041240844No Hit
CCGGGGGGGCGTCGTCACGCGCTCTCCCTCCTGGAATTCTCGG1125640.11073369493709126No Hit
TACTCTGGCATGCTAACTAGTTACGCGACCCTGGAATTCTCGG1118780.11005884938498896No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGG1091550.10738012571388895No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGT1071520.10540969475053483No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGT1063080.10457941829867719No Hit
GCAAAAGCTCGCTTGATCTTGATTTTCAGTGGAATTCTCGGGT1060320.10430790609404125No Hit
ACCCTACGCCGGCAGGCGCGGGTAACCCGTTGTGGAATTCTCG1058410.10412001177851422No Hit
AAATGCGAGTGCCTAGTGGGCCACTTTTGGTGGAATTCTCGGG1055580.10384161339477523No Hit
TCCGCGGCGCGGGAAATGTGGCGTACGGAAGTGGAATTCTCGG1036770.10199119869578915No Hit
CTAATACATGCCGACGGGCGCTGACCCCCCTTGGAATTCTCGG1031300.10145309298587667No Hit
GGGGGGCCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCG1029820.10130749948484001No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCTGGAATTCTCG1029540.10127995476842769No Hit
TAACCCCATTCGTGATGGGGATCGGGGATTGTGGAATTCTCGG1027790.10110780029085056No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCCTGGAATTCTCGGG1019360.10027850757886476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)