FastQCFastQC Report
Fri 14 Jul 2023
SRR8919683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8919683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87510149
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length43
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG25961702.9667073244270217No Hit
TCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT21689662.478530804467034No Hit
ACCCCGCCCCGAGTGTTACAGCCCCCCGGCTGGAATTCTCGGG17119331.9562679524177247No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG14702371.6800759875291722No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGG11264191.2871867010533828No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG10369781.1849802701170122No Hit
TCGGGTCTTCCCGGAGTCGGGTTGCTTGGGTGGAATTCTCGGG7495240.8564995129879164No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGT7398770.8454756487730355No Hit
TACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGG5061650.5784071970897913No Hit
TTGGTAATTCTAGAGCTAATACATGCCGACTGGAATTCTCGGG4462880.5099842762237784No Hit
CGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTG4360670.4983044880885759No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGT4265140.48738803998608204No Hit
TACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTCGG4187610.47852849616334214No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGG3660220.4182623434911532No Hit
GCGACCCCGGGGGCCGGGCGGCGTCGGCTTGGAATTCTCGGGT3587580.4099615919977465No Hit
TACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCG3331950.3807501230514417No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGGTGGAATTCTCGGG3132400.3579470536611702No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGG3069060.35070903604563625No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGGCTGGAATTCTCGG3044190.3478670799657763No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGGT2999530.342763671902787No Hit
TGGTAATTCTAGAGCTAATACATGCCGACTGGAATTCTCGGGT2948120.3368889247348899No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG2838390.32434980770059024No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGT2745680.31375560793525786No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGTGGAATTCTCGGGT2728770.31182326063688914No Hit
TCGCCGCGCTCTCCCCCCGGCCTCTCCCCTTGGAATTCTCGGG2685850.30691868665427596No Hit
TACCCCGCCCCGAGTGTTACAGCCCCCCGGCTGGAATTCTCGG2532190.289359580452777No Hit
TCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGGTG2408210.27519208086367214No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGTGGAATTCTCGG2377290.2716587764008949No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGTGGAATTCTCGGG2339310.2673187083706142No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCG2277660.2602738112124572No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTTGGAATTCTCGG2272280.2596590253777308No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCG2242120.2562125679845431No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTGGAATTCTCGGG2228940.25470645696192334No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG2221970.25390997791581865No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTTGGAATTCTCGGGTG2191030.2503743880038417No Hit
TCGACAACCCGCAGTTTTATCCGGTAAAGCGTGGAATTCTCGG2167180.24764898983316783No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCG2138570.244379654752959No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGTG2136780.24417510704958348No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGTGGAATTCTCGGG2112420.24139142992431656No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGG2086760.23845919860106737No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGG2041950.2333386496690801No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGG2027360.2316714144778796No Hit
TCGGGTCTTCCCGGAGTCGGGTTGCTTGGTGGAATTCTCGGGT2007360.22938596527815303No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGTGGAATTCTCGGGT1980430.22630860793072127No Hit
CCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT1914400.21876319739782413No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGG1881220.2149716371754778No Hit
TTGGGGGGGACGCGTGCATTTATCAGATTGGAATTCTCGGGTG1878110.21461624982492034No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGT1854260.21189085165424643No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGG1823570.20838382985726603No Hit
ATACCGTCGTAGTTCCGACCATAAACGATGTGGAATTCTCGGG1803960.2061429469169342No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGTGGAATTCTCGG1786330.20412832344737522No Hit
GCGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT1781940.20362666734803522No Hit
TATACCGTCGTAGTTCCGACCATAAACGATGTGGAATTCTCGG1712210.1956584487131887No Hit
GGGGGGCCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCG1650780.1886386914962286No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGTGGAATTCTCGGGTG1649210.1884592837340501No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGT1645070.18798619574970668No Hit
AGTGCCGTTCCGAAGGGACGGGCGATGGCTGGAATTCTCGGGT1594120.18216401391340337No Hit
CCGGGGGGGCGTCGTCACGCGCTCTCCCTCCTGGAATTCTCGG1577770.18029565919262688No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGGT1561310.17841473450125198No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG1548310.17692919252142972No Hit
AAATACATGCCGACGGGCGCTGACCCCCCTTGGAATTCTCGGG1526360.17442091202472984No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGT1483620.16953690708491423No Hit
GGGGGGGGGCGTCGTCACGCGCTCTCCCTCCCCTTGGAATTCT1468890.16785367374931565No Hit
GGGGGGGGCGTCGTCACGCGCTCTCCCTCCCCTTGGAATTCTC1451390.1658539056995549No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGT1419460.1622051860521915No Hit
AAATTGGAATGAGTCCACTTTAAATCCTTTTGGAATTCTCGGG1403910.16042824929940414No Hit
TCGGGGGGGCGTCGTCACGCGCTCTCCCTCCCCTTGGAATTCT1392530.15912782870475972No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGG1356110.1549660257120577No Hit
CTAATACATGCCGACGGGCGCTGACCCCCCTTGGAATTCTCGG1355510.1548974622360659No Hit
AAAAGGGGGCCGCCCTCTCGCCCGTCACGCTGGAATTCTCGGG1349890.15425525101094273No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGTGGAATTCTCGGG1331190.15211835600919843No Hit
TTTTATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTCGGG1326790.15161555718525857No Hit
GGGGGCCCCGAGGTGGGATCCCGAGGCCTCTCCTGGAATTCTC1322270.1510990456661204No Hit
TTGGGGGGGACGCGTGCATTTATCAGATCTGGAATTCTCGGGT1315910.15037227282060736No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGG1280470.14632245683869194No Hit
GGGGGGCCCCGAGGTGGGATCCCGAGGCCTCTCTGGAATTCTC1279960.1462641778840989No Hit
TGCGCTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGG1263860.14442439127831905No Hit
ACCGGGGTCCGGTGCGGAGAGCCATTCGTGGAATTCTCGGGTG1260470.1440370076389654No Hit
ACCCCGCCCCGAGTGTTACAGCCCCCCGGTGGAATTCTCGGGT1247440.14254803748534356No Hit
TCTAGATAACCTCGGGCCGATCGCACGCCCTTGGAATTCTCGG1241610.14188182904362326No Hit
TTGAAACTGCGAATGGCTCATTAAATCAGTTTGGAATTCTCGG1228450.1403780034702032No Hit
AGATACCGTCGTAGTTCCGACCATAAACGTGGAATTCTCGGGT1201780.13733035696236787No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTGGAATTCTCGGG1197150.13680127547263118No Hit
TTTTTATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTCGG1171740.13389761226437863No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA1168690.13354908126142032Illumina Paired End PCR Primer 2 (100% over 22bp)
GCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGGTG1139320.1301928991116219No Hit
TAATTGGAATGAGTCCACTTTAAATCCTTTTGGAATTCTCGGG1126930.1287770633323913No Hit
TTTGGTAATTCTAGAGCTAATACATGCCGACTGGAATTCTCGG1123510.12838625151923808No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCG1102390.12597281716432684No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGT1084480.12392619740597174No Hit
ACCCTACGCCGGCAGGCGCGGGTAACCCGTTGTGGAATTCTCG1072730.1225834960011324No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG1057720.12086826637673763No Hit
TCGCCGAAGGAGCCTCGGTTGGCCCCGGATTGGAATTCTCGGG1031410.11786175795449737No Hit
CGGGGGGGCGTCGTCACGCGCTCTCCCTCCCCTTGGAATTCTC998080.1140530568631531No Hit
TTAATTGGAATGAGTCCACTTTAAATCCTTTTGGAATTCTCGG986360.11271378363211335No Hit
TAGTTGGATCTTGGGAGCGGGCGGGCGGTTGGAATTCTCGGGT986140.11268864369091634No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGG977620.11171504233183284No Hit
TAACCTATTCTCAAACTTTAAATGGGTAAGTGGAATTCTCGGG972490.111128824612103No Hit
TGAAACTGCGAATGGCTCATTAAATCAGTTTGGAATTCTCGGG966900.11049004156077943No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGG935000.1068447500872156No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTCGGGTG930580.10633966581407603No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGG922010.10536035083199322No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCTGGAATTCTCGGG915660.10463472071108003No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTGGAATTCTCGGG895510.10233213064235555No Hit
TGGGGCCCCGAGGTGGGATCCCGAGGCCTCTCCTGGAATTCTC889660.10166363675143553No Hit
ACGGGGGCCCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT886160.1012636831414834No Hit
CGCTTCTGGCGCCAAGCGCCCGTCCGCGCTGGAATTCTCGGGT881250.10070260536295055No Hit
GCGGGTCTTCCCGGAGTCGGGTTGCTTGGGTGGAATTCTCGGG879120.10045920502317965No Hit
TGGCGCTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGG876290.10013581396141834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)