FastQCFastQC Report
Fri 14 Jul 2023
SRR9002961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9002961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95591247
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT1547184016.185414967962494No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1299592813.595311713006527No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG63563336.649492709306323No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCAAGATCGGAAGAGCACACGT42772714.474542527936685No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCT41285334.31894459960335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCA19260442.0148748556444716No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACACG14268741.4926826930084927No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG10332361.0808897597078109No Hit
AACCAACTTAGAACTGGTACGGACAGGGGGAAAGATCGGAAGAGCACACG10128321.0595447091510377No Hit
TGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTG9681331.0127841516702885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGTCT8245170.8625444545147527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTC8186200.8563754796503491No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTGA7478170.7823069825629537Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGACCAGCGAATCCCTGGTGTTGTATGTAGATCGGAAGAGCACACGTCT5716960.5980631260098531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCAC5493790.5747168461982717No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTAGATCGGAAGAGCAC4977360.5206920252855369No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCAAGATCGGAAGAGCACACG4927410.5154666514602535No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGAAGATCGGAAGAGCACACGTC4727970.4946028165110138No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACACG4626730.4840118886617307No Hit
GATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACGT4276080.44732965979615263No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACAC4175490.43680672980445584No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTCAGATCGGAAGAGC3914030.40945485312060004No Hit
CAACCAACTTAGAACTGGTACGGACAGGGGGAAAGATCGGAAGAGCACAC3634220.38018334461103953No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACG3314960.3467848892064354No Hit
AGACGTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACA3197230.3344689080162329No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGAGATCGGAAGAGCACACGTCT3185800.3332731918436005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCAAGGATGTTTTCATTAATCAGGAACAGATCGGAAGAGCACACGTCTG3168900.33150524754635746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCAACTTAGAACTGGTACGGACAGGGGGAATAGATCGGAAGAGCACAC3150730.3296044458965997No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACGT3076900.32188093539568535No Hit
ATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACGTC2746550.2873223319285708No Hit
GCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA2688670.2812673842407349Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCGGCAACAGCTCAAATTTGAAATCTGGCAGATCGGAAGAGCACACGT2344940.2453090710282292No Hit
GCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTCAGATCGGAAGAGCA2120730.22185399464450967No Hit
CGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTCT2080740.2176705572216251Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCAGATCGGAAGAGCACA2066400.21617041987118338No Hit
AACTATGCCGATTAGGGATCGGACGGTGAGATCGGAAGAGCACACGTCTG1897670.19851922216267354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGACCAGCGAATCCCTGGTGTTGTATGTAGATCGGAAGAGCACACGTC1882790.19696259428439092No Hit
AGACGTATAAGAATAGGCTCCGGCCTCGTATCTTAAGATCGGAAGAGCAC1826670.19109176387248092No Hit
CGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGTCTG1803080.18862396470254228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACACGT1634210.17095812130162924No Hit
AATGGCTCAGTGAGGCTTCCGGACTGGCCAGATCGGAAGAGCACACGTCT1527200.1597635817011572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTACTCAGACGGAAAAATTCGCCCAAAAGATCGGAAGAGCACACGTCT1507710.15772469209445505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGGCGAGCATGCCTGTTCGAGCGTCAGATCGGAAGAGCACACGTCTG1421340.1486893460025686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGATTCATAATAACTTCTCGAATCGCAGATCGGAAGAGCACACGTC1404150.14689106419963324No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACAC1390510.14546415531120752No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCTCGAGATCGGAAGAGCAC1387980.14519948672706404No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACAC1387720.14517228758402953No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACGTC1380300.1443960658866601No Hit
CGTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACACGT1378500.1442077641271904No Hit
GTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCACACGTCTGAA1334110.13956403351449112Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGGCGAGCATGCCTGTTCGAGCGTCAGATCGGAAGAGCACACGTCTGA1192200.12471853202207939Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACG1185000.1239653249842007No Hit
GTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACACGTC1176290.12305415369254467No Hit
TAGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGC1140270.11928602626137935No Hit
GAAGCGGCAACAGCTCAAATTTGAAATCTGGCAGATCGGAAGAGCACACG1134310.11866253821335754No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCCAAGATCGGAAGAGCACACGT1072350.11218077320405706No Hit
AACCAACTTAGAACTGGTACGGACAGGGGGAAGATCGGAAGAGCACACGT1068510.11177906278385509No Hit
AGCGGAGGAAAAGAAACCAACAGGGATTGCCCAGATCGGAAGAGCACACG1056360.11050802590743482No Hit
GGGAATGCTGCTCAAAATGGGAGGTAAGATCGGAAGAGCACACGTCTGAA1040900.10889072301776752Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGCTGAGGGTAAGCCCTCCTTCGCCTAGATTTCAGATCGGAAGAGCACA1032120.1079722288799099No Hit
CCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCACA1007730.10542074003909584No Hit
ATCGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACAC1002480.10487152657397596No Hit
GCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTAGATCGGAAGAGCACA1000390.10465288730881395No Hit
ATGCTCGAATGTACTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG976290.10213173597369224No Hit
AGCGGCAACAGCTCAAATTTGAAATCTGGCAGATCGGAAGAGCACACGTC963740.10081885426183425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)