FastQCFastQC Report
Fri 14 Jul 2023
SRR9002962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9002962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97486737
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1755873518.011409080191083No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGT1122098611.510269340536036No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCT58255775.975763656957767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG47207794.842483342118631No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACACG26890242.7583485536088874No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTGA23011502.3604749433761434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGATTCATAATAACTTCTCGAATCGCAAGATCGGAAGAGCACACGT20153852.0673427606875383No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACAC14643001.502050478928226No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCA14004011.4365041267100775No Hit
TGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTG13928781.4287871795319194Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGACCAGCGAATCCCTGGTGTTGTATGTAGATCGGAAGAGCACACGTCT9460150.9704037996471253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTC8105990.831496698879151No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCAAGATCGGAAGAGCACACG6401560.6566595823183619No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACG6013990.6169034050242137No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACGTC4781280.4904544091982482No Hit
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTAGATCGGAAGAGCAC4461130.4576140444622739No Hit
AGACGTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACA4392230.4505464163807226No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACGT4039310.41434456873861725No Hit
GCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTAGATCGGAAGAGCAC3960070.4062162835545517No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGTCT3930550.4031881793315126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACACG3892410.39927585226285706No Hit
AACCAACTTAGAACTGGTACGGACAGGGGGAAAGATCGGAAGAGCACACG3455420.35445026742458313No Hit
GACGACCAGCGAATCCCTGGTGTTGTATGTAGATCGGAAGAGCACACGTC3097560.3177416841841778No Hit
CGTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACACGT2783520.28552807137241654No Hit
GATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACGT2663950.27326281317631956No Hit
GTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACACGTC2489080.2553249884648411No Hit
CGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTCT2460930.2524374161789824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTCAGATCGGAAGAGC2348890.24094457074709555No Hit
GCCAAGGATGTTTTCATTAATCAGGAACAGATCGGAAGAGCACACGTCTG2305520.23649576044380274Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGAGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA2134200.21892208783231712Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTTAGATCGGAAGAGCACACG2131130.21860717319936557No Hit
ATCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGTC1880250.19287239042578685No Hit
CGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACACGT1771470.18171394945755545No Hit
ATCTCTTGGTTCTGGCATCGATGAAGAACGAGATCGGAAGAGCACACGTC1762770.18082152036743213No Hit
CATCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGT1554940.1595027229191187No Hit
ATCGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGT1506700.1545543574814695No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCTCGAGATCGGAAGAGCAC1482780.1521006903739121No Hit
CATCGAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACG1474020.15120210660040861No Hit
GACATTGTCAGGTGGGGAGTTTGGCTGGGAGATCGGAAGAGCACACGTCT1392930.1428840520121214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTCAGATCGGAAGAGCA1340870.1375438383992686No Hit
GAATCTTTGAACGCACATTGCGCTCGCCAGATCGGAAGAGCACACGTCTG1315250.13491578859593997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTATAAGAATAGGCTCCGGCCTCGTATCTTAAGATCGGAAGAGCAC1292170.13254828705570482No Hit
GAATGGCTCAGTGAGGCTTCCGGACTGGCCAGATCGGAAGAGCACACGTC1275420.13083010461207661No Hit
ACGACCAGCGAATCCCAGGTGTTGTATGTAGATCGGAAGAGCACACGTCT1266480.12991305678843268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAATCATCGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCA1262850.12954069844393293No Hit
ATGCTCGAATGTACTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG1250080.12823077666452207No Hit
GGGTACTCAGACGGAAAAATTCGCCCAAAAGATCGGAAGAGCACACGTCT1235180.12670236362511547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTATAAGAATAGGCTCCGGCCTCGTATCTTAGATCGGAAGAGCACAC1198880.12297878018011825No Hit
GCGAAAGGGTCCTGTAAGCAGTAGAGTAGATCGGAAGAGCACACGTCTGA1192080.12228124939703336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTATAAGAATAGGCTCCGGCCTCGTATCTTAAGATCGGAAGAGCACACG1180690.1211128853353662No Hit
GGTGATTCATAATAACTTCTCGAATCGCATAGATCGGAAGAGCACACGTC1152980.11827044739429529No Hit
CGAATCTTTGAACGCACATTGCGCTCGCAGATCGGAAGAGCACACGTCTG1122980.11519310570421491Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAAGAATAGGCTCCGGCCTCGTATCTTAGAGATCGGAAGAGCACACG1113300.11420015011888232No Hit
GTTACGATCTGCTGAGGGTAAGCCCTCCTTCGCCTAGATCGGAAGAGCAC1097140.11254248872849237No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCAGATCGGAAGAGCACACGTCTGA1078980.11067967122543039Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGAATCTTTGAACGCACATTGCGCTCGCCAGAGATCGGAAGAGCACAC1037490.10642370766804925No Hit
CTGCTGAGGGTAAGCCCTCCTTCGCCTAGATTTCAGATCGGAAGAGCACA1033120.10597544156186088No Hit
TCGAATCTTTGAACGCACATTGCGCTCGCCAAGATCGGAAGAGCACACGT1019380.10456601906780405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)