FastQCFastQC Report
Fri 14 Jul 2023
SRR9005126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9005126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49805294
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTC26902625.401558316270556No Hit
GAGAGTCGAACCTGATAGTATGGAGCAGATCGGAAGAGCACACGTCTGAA22663294.550377716874836Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGT20723344.1608709307086915No Hit
GTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAGT8591271.7249712450246752No Hit
GAGAGTCGAACCTGATAGTATGGAAGATCGGAAGAGCACACGTCTGAACT6577281.3205985693006852Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATGTTACGGCATGTGTGGGGTTGTAGGACCACAGATCGGAAGAGCACAC6516701.3084352036954143No Hit
AGTGACGTGGAGACGGAGGCGTGACAGCGCCGCGGACTGACGGAATAGTC5391091.0824331244786949No Hit
GAGAGTCGAACCTGATAGTATGGAGCGTTAGATCGGAAGAGCACACGTCT4775870.9589081032229224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAACCTTCGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAA4766830.9570930351299602No Hit
TGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAGTA4643720.9323747792754722No Hit
GAGAGTCGAACCTGATAGTATGGAGCGTTCTTCGGAACAATCCAATAGTG4619990.9276102255314466No Hit
ATGGTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGT4041250.8114097268455036No Hit
GGAGTGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAG3904660.7839849314010674No Hit
AGGAACCTTACCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3893350.781714088466178Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCC3532510.7092639589678961No Hit
GAGAGTCGAACCTGATAGTATGGAGCGAGATCGGAAGAGCACACGTCTGA2925660.5874194819530631Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTACCCGTACCGCAAACGGACACACGTAGTCGGGTTGAATATACTAAG2761840.5545273962241846No Hit
GAGAGTCGAACCTGATAGTATGGAGTAGATCGGAAGAGCACACGTCTGAA2691480.5404003839431206Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTGACGTGGAGACGGAGGCGTGACAGCGCCGCGGACTGACGGAATAGT2684080.5389145981148109No Hit
AATGGGCGCAGGCCTGAACCAGCCAAGTAGCGTGAAGGATGACTGCCCTA2406470.4831755435476397No Hit
CTGATGAATAGAATCCTCTGGAAAGTGGAGATCGGAAGAGCACACGTCTG2287920.4593728530143804Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGTTACGGCATGTGTGGGGTTGTAGGACCAAGATCGGAAGAGCACACG2237270.4492032513652063No Hit
AGCAAGGGCGCATGGCGGATGCCTTGGCTCTCGGAGGCGATGAAGGACGT2203600.44244292584639694No Hit
GGAGTGACGTGGAGACGGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1973730.3962891976905106Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGAGTCGAACCTGATAGTATGGAGATCGGAAGAGCACACGTCTGAACTC1915940.38468601349888626Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTG1825080.366442972909667No Hit
CGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCCGTTG1728340.3470193349325475No Hit
ATGTTACGGCATGTGTGGGGTTGTAGGACCACAGATCGGAAGAGCACACG1688590.33903825565209994No Hit
TGGTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTA1614490.3241603191821335No Hit
AGCGCCGCGGACTGACGGAATAGTCCGTTGAAGGGTGTAGATGTTGATCA1604960.32224686797351304No Hit
CAAAAGCCTCTCTCAGTTCGGATCGAAGTCTGCAACCCGACTTCGTGAAG1425560.28622660073043643No Hit
GGTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAG1407090.2825181596157228No Hit
TACAATGAAGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTT1373160.275705630811054No Hit
CCACGTGTGGAATTTTGTATGTACCATATGAATAAGGATCGGCTAACTCC1355300.27211966663624154No Hit
AGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCAA1316370.26430322848812016No Hit
AGAGTCGAACCTGATAGTATGGAGCAGATCGGAAGAGCACACGTCTGAAC1313730.2637731643547772Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCCGTTGA1304040.2618275880471662No Hit
GAGAGTCGAACCTGATAGTATGGAGCGTAGATCGGAAGAGCACACGTCTG1284790.25796253707487404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCCGATTGCGAAGGCAGCTCACTGGACTGCAACTGACACTGATGCTC1235100.24798568601964283No Hit
GAGTGACGTGGAGACGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1216590.24426921363018156Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTATTAAAGAATTTCGGTTATCGATGGGGATGCGTTCCATTAGGCAGTTG1193490.2396311524634309No Hit
AAACGGACACACGTAGTCGGGTTGAATATACTAAGGCGCTTGAGTGATTC1155870.23207773856329408No Hit
ACAAGCGGAGGAACATGTGGTTTAATTCGAAGATCGGAAGAGCACACGTC1127860.22645383842127306No Hit
GCGACCGGCGCACGGGTGAGTAACACGTATCCAACCTGCCGATAACTCGA1111900.22324935979697258No Hit
GAGAGTCGAACCTGATAGTATGGAGAGATCGGAAGAGCACACGTCTGAAC1102640.2213901196928985Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCATGGTCTTGTTATTAAAGAATTTCGGTTATCGATGGGGATGCGTTCC1048820.2105840395199755No Hit
GTGACGTGGAGACGGAGGCGTGACAGCGCCGCGGACTGACGGAATAGTCC1047410.2103009370851219No Hit
ATCCAATAGTGCGTGTAAGCATACTCCCAAGAAAATCCGCTAAACTAGAT1038400.20849189244822047No Hit
TCTGATGAATAGAATCCTCTGGAAAGTGGAGATCGGAAGAGCACACGTCT951710.19108611225144057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCC923830.18548831375234928No Hit
ACAAGCGGAGGAACATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCT918520.18442216202960274Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAGA915030.18372143330787286No Hit
GAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCA906090.18192644340177974No Hit
AATCCAATAGTGCGTGTAAGCATACTCCCAAGAAAATCCGCTAAACTAGA878070.17630053544107177No Hit
ACTGGAGGACCGAACCGATAAGCGTTGAAAAGCTTCCGGATGAACTGAGG870120.17470431958498228No Hit
GTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTAGATC856170.1719034125167497No Hit
AATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAGTATCG835220.16769703236768366No Hit
AATGATCGGTAGGGGAGCATTCCACTCTGCGTCGAAGGTGAAGCGTGAGC826620.16597030829694528No Hit
AAGTCTTGATAAACTCCGAATGCGCATTAGTTCTATCAGATCGGAAGAGC819690.1645788899469201No Hit
ATTTAGGTGCAGCCTTGTGAGTTACTAATGTGAGGTAGAGCGACTGATAA794130.15944690538319078No Hit
CAAGCGGAGGAACATGTGGTTTAATTCGAAGATCGGAAGAGCACACGTCT785920.15779848624124174Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAACATGTGGTTTAATTCGAAGATCGGAAGAGCACACGTCTGAACTCC778030.15621431729727367Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCAAGACATCTGATGAATAGAATCCTCTGGAAAGTGGAGATCGGAAG768540.15430889736340075No Hit
ATGGTGTAATGATCTGGACACTGTCTCAGATCGGAAGAGCACACGTCTGA751920.15097190270576458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGACGTGGAGACGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT746770.14993787608200845Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGAACCTTACCCGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC734650.14750439983347957Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCAAGACATCTGATGAATAGAATCCTCTGGAAAGTGGAGATCGGAAGA720620.14468743021575176No Hit
ACGTGTGGAATTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC706170.14178613221317396Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACGTGTGGAATTTTGTATGTACCATATGAATAAGGATCGGCTAACTCCG701500.1408484808863893No Hit
TTGATGAGTAGAATCCTCTGGAAAGTTGAGATCGGAAGAGCACACGTCTG691110.13876235727069494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGACGGGAGTCTGAACCAGCCAAGTAGCGTGAAGGATGACTGCCCTA689450.1384290593686687No Hit
CAGTAAGCGGCCAAGCGAAAGCATTAAGTATTCCACCTGGGGAGTACGCC685500.1376359709873412No Hit
AAGGTACCCGTACCGCAAACGGACACACGTAGTCGGGTTGAATATACTAA648710.13024920603821755No Hit
GAGAGTCGAACCTGATAGTATGGAACGTTCTTCGGAACAATCCAATAGTG647250.12995606450992941No Hit
TGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCCG646760.12985768139427106No Hit
GAGTGACGTGGAGACGGAGCAGTGACAGATCGGAAGAGCACACGTCTGAA638150.1281289495048458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGACCGGCGCACGGGTGAGTAACACGTATCCAACCTGCCGATAACTCG625010.12549067575025258No Hit
CCATTAAAGTGGCACGCGAGCTGGGTTCAGAACGTAGATCGGAAGAGCAC615730.1236274200088047No Hit
GTGTAATGATCTGGACACTGTCTCAGATCGGAAGAGCACACGTCTGAACT612290.12293673038050935Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCGAACCTGATAGTATGGAGCGTTCTTCGGAACAATCCAATAGTGCGT600170.12050325413198043No Hit
CATGTTACGGCATGTGTGGGGTTGTAGGACCATAGATCGGAAGAGCACAC598180.12010369821328633No Hit
GGAGTGACGTGGAGACGGAGCAGTGACAGATCGGAAGAGCACACGTCTGA592090.11888093663296115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGAAGGAATGTCATCCATCTTTGATGGAAGCTAACGCAGGGAACTGAA591080.11867814694558373No Hit
AAACCTTCGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAAC583960.11724858004050734No Hit
AGTGACGTGGAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC583690.1171943689359609TruSeq Adapter, Index 5 (100% over 35bp)
TAATCCTTAAGGTACCCGTACCGCAAACGGACACACGTAGTCGGGTTGAA583440.11714417346878829No Hit
AAGTCTTGATAAACTCCGAATGCGCAGATCGGAAGAGCACACGTCTGAAC573930.11523473789754157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACGTGTGGAATTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA570570.11456011081874147Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTATTAAAGAATTTCGGTTATCGATGGGGATGCGTTCCATTAGGCAGTT567900.11402402322933784No Hit
ATGATCGGTAGGGGAGCATTCCACTCTGCGTCGAAGGTGAAGCGTGAGCT553750.11118295978736717No Hit
GAGAGTCGAACCTGATAGTATGGAGAAGATCGGAAGAGCACACGTCTGAA552780.11098820137473739Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGATGAGTAGAATCCTCTGGAAAGTTGAACCATAGACGGTGATAGTCCG547740.10997626075653724No Hit
AGGACTCTAGAGAGACTGCCGTCGTAAGATGTGAGGAAGGTGGGGATGAC541440.10871133498378707No Hit
AGTGACGTGGAGACGGAGCAGTGACAGATCGGAAGAGCACACGTCTGAAC541230.10866917079136205Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCGTCGAGTCGGAAGGAGTAGACGCGAAACCAAGTGATCTACCCTTG533570.1071311816771928No Hit
TTTGATGAGTAGAATCCTCTGGAAAGTTGAACCATAGACGGTGATAGTCC532690.10695449363274515No Hit
ACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCCGTT531730.10676174303880226No Hit
AGGAACCTTACCCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA520480.10450294701603409Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTGACAGCGCCGCGGACTGACGGAATAGTCCGTTGAAGGGTGTAGATGT519240.10425397749885786No Hit
AGGAACATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCTGAACTCCA519020.10420980548774593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGGAGTCTGAACCAGCC518400.10408532072915783No Hit
ACTAAGTTGAACTAACGAAGTCAGATTGCTAAGACAGCTAGGATGTTGGC512980.10299708300085528No Hit
CAAGCGGAGGAACATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCTG506800.10175625105234797Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCACGTGTGGAATTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC499020.10019416811393583Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAGTCGAACCTGATAGTATGGAAGATCGGAAGAGCACACGTCTGAACTC498390.10006767553666082Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)