FastQCFastQC Report
Fri 14 Jul 2023
SRR9005127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9005127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15102379
Total Bases755.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTC7061994.676077855018736No Hit
GAGAGTCGAACCTGATAGTATGGAGCAGATCGGAAGAGCACACGTCTGAA6933884.5912501599913496Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGT5762273.8154717213758174No Hit
GTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAGT2473071.6375367086205426No Hit
CATGTTACGGCATGTGTGGGGTTGTAGGACCACAGATCGGAAGAGCACAC2245611.486924675907021No Hit
GAGAGTCGAACCTGATAGTATGGAAGATCGGAAGAGCACACGTCTGAACT2073201.3727638539596974Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGAGTCGAACCTGATAGTATGGAGCGTTCTTCGGAACAATCCAATAGTG1997951.3229372670358757No Hit
CAAACCTTCGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAA1747741.1572613824616638No Hit
GAGAGTCGAACCTGATAGTATGGAGCGTTAGATCGGAAGAGCACACGTCT1681791.1135927657490254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAGTA1395950.9243245716453017No Hit
AGGAACCTTACCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1219520.8075019174131439Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTACCCGTACCGCAAACGGACACACGTAGTCGGGTTGAATATACTAAG1198230.7934048006608759No Hit
ATGGTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGT1145070.7582050483569509No Hit
AGTGACGTGGAGACGGAGGCGTGACAGCGCCGCGGACTGACGGAATAGTC1108050.7336923540324342No Hit
GGAGTGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAG1071940.7097822137823452No Hit
GTGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCC1042570.6903349465670277No Hit
CATGTTACGGCATGTGTGGGGTTGTAGGACCAAGATCGGAAGAGCACACG809980.5363260980273373No Hit
GAGAGTCGAACCTGATAGTATGGAGCGAGATCGGAAGAGCACACGTCTGA781640.5175608425665916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGGCGCAGGCCTGAACCAGCCAAGTAGCGTGAAGGATGACTGCCCTA725790.4805799139327651No Hit
AGCGCCGCGGACTGACGGAATAGTCCGTTGAAGGGTGTAGATGTTGATCA630980.41780172514542246No Hit
CGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCCGTTG605720.40107588347504725No Hit
ATGTTACGGCATGTGTGGGGTTGTAGGACCACAGATCGGAAGAGCACACG604470.40024819930687744No Hit
GAGTGACGTGGAGACGGAGGCGTGACAGCGCCGCGGACTGACGGAATAGT590550.39103110841013855No Hit
AGCAAGGGCGCATGGCGGATGCCTTGGCTCTCGGAGGCGATGAAGGACGT584410.3869655237760885No Hit
GAGAGTCGAACCTGATAGTATGGAGCGTAGATCGGAAGAGCACACGTCTG559820.3706833208198523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGAGTCGAACCTGATAGTATGGAGTAGATCGGAAGAGCACACGTCTGAA546390.361790682117036Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTG544700.36067165312167043No Hit
GTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCCGTTGA515030.34102574170599215No Hit
GGAGTGACGTGGAGACGGAGCAGATCGGAAGAGCACACGTCTGAACTCCA512190.3391452432759104Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGACCGGCGCACGGGTGAGTAACACGTATCCAACCTGCCGATAACTCGA501250.33190135143608834No Hit
AACTCCGATTGCGAAGGCAGCTCACTGGACTGCAACTGACACTGATGCTC496270.3286038577100998No Hit
TGGTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTA459690.30438250821277896No Hit
AGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCAA439370.2909276743750107No Hit
CAAAAGCCTCTCTCAGTTCGGATCGAAGTCTGCAACCCGACTTCGTGAAG433600.28710708425473896No Hit
AGAGTCGAACCTGATAGTATGGAGCAGATCGGAAGAGCACACGTCTGAAC430510.2850610489910232Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGGAGGACCGAACCGATAAGCGTTGAAAAGCTTCCGGATGAACTGAGG427040.2827633977401839No Hit
ACAAGCGGAGGAACATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCT422330.27964468379452Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACAATGAAGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTT420780.2786183554259895No Hit
CCACGTGTGGAATTTTGTATGTACCATATGAATAAGGATCGGCTAACTCC415430.27507586718622273No Hit
GGTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAG405320.26838155763406546No Hit
CTGATGAATAGAATCCTCTGGAAAGTGGAGATCGGAAGAGCACACGTCTG388670.2573568045140438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGACGTGGAGACGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAG388370.25715816031368305Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAAGCGGAGGAACATGTGGTTTAATTCGAAGATCGGAAGAGCACACGTC382520.2532845984066484No Hit
ATCCAATAGTGCGTGTAAGCATACTCCCAAGAAAATCCGCTAAACTAGAT381370.2525231289719322No Hit
AAACGGACACACGTAGTCGGGTTGAATATACTAAGGCGCTTGAGTGATTC370420.24527261565876474No Hit
GTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAGA348290.2306192951454867No Hit
AATCCAATAGTGCGTGTAAGCATACTCCCAAGAAAATCCGCTAAACTAGA343610.22752044561985896No Hit
CAGTAAGCGGCCAAGCGAAAGCATTAAGTATTCCACCTGGGGAGTACGCC332590.22022358199327405No Hit
GAGAGTCGAACCTGATAGTATGGAGATCGGAAGAGCACACGTCTGAACTC322020.21322468466723027Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCATTAAAGTGGCACGCGAGCTGGGTTCAGAACGTAGATCGGAAGAGCAC309260.20477568467855298No Hit
GAGAGTCGAACCTGATAGTATGGAGAGATCGGAAGAGCACACGTCTGAAC305350.20218668860051783Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGACCGGCGCACGGGTGAGTAACACGTATCCAACCTGCCGATAACTCG298370.1975649002054577No Hit
TTATTAAAGAATTTCGGTTATCGATGGGGATGCGTTCCATTAGGCAGTTG293250.19417470585263422No Hit
GGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCC292070.19339337199788192No Hit
GAGAGTCGAACCTGATAGTATGGAACGTTCTTCGGAACAATCCAATAGTG290310.19222799268909885No Hit
GAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCA285910.18931454441714116No Hit
CGCATGGTCTTGTTATTAAAGAATTTCGGTTATCGATGGGGATGCGTTCC281950.18669244097237925No Hit
ATTTAGGTGCAGCCTTGTGAGTTACTAATGTGAGGTAGAGCGACTGATAA278900.18467289160204495No Hit
AAGGTACCCGTACCGCAAACGGACACACGTAGTCGGGTTGAATATACTAA254160.16829136654562835No Hit
GTGACGTGGAGACGGAGGCGTGACAGCGCCGCGGACTGACGGAATAGTCC250880.16611952328835078No Hit
AAGTCTTGATAAACTCCGAATGCGCATTAGTTCTATCAGATCGGAAGAGC249680.1653249464869078No Hit
AGGAACATGTGGTTTAATTCGAAGATCGGAAGAGCACACGTCTGAACTCC248520.16455685557884622Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGACAGCGCCGCGGACTGACGGAATAGTCCGTTGAAGGGTGTAGATGT247970.1641926745448515No Hit
AATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTGTAGTATCG246320.1631001314428674No Hit
CAAGCGGAGGAACATGTGGTTTAATTCGAAGATCGGAAGAGCACACGTCT243630.16131895511296598Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGATCGGTAGGGGAGCATTCCACTCTGCGTCGAAGGTGAAGCGTGAGC242860.16080910166537338No Hit
AGGGCGTCGAGTCGGAAGGAGTAGACGCGAAACCAAGTGATCTACCCTTG237450.15722688458553452No Hit
AAACCTTCGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAAC234560.15531327878872594No Hit
AGGAACCTTACCCGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC231720.15343278035864416Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAATCCTTAAGGTACCCGTACCGCAAACGGACACACGTAGTCGGGTTGAA226860.1502147443128No Hit
AAGTCTTGATAAACTCCGAATGCGCAGATCGGAAGAGCACACGTCTGAAC221880.14691725058681152Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CATTAAAGTGGCACGCGAGCTGGGTTCAGAACGTAGATCGGAAGAGCACA214240.14185844495095773No Hit
AGTGACGTGGAGACGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT212780.1408917098425354Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTCGAACCTGATAGTATGGAGCGTTCTTCGGAACAATCCAATAGTGCGT209460.13869338069187642No Hit
TGGCGACCGGCGCACGGGTGAGTAACACGTATCCAACCTGCCGATAACTC206520.13674666752834108No Hit
AGTAAGCGGCCAAGCGAAAGCATTAAGTATTCCACCTGGGGAGTACGCCG206250.1365678877480164No Hit
CACGTGTGGAATTTTGTATGTACCATATGAATAAGGATCGGCTAACTCCG204420.1353561581258158No Hit
GAGTGACGTGGAGACGGAGCAGTGACAGATCGGAAGAGCACACGTCTGAA203390.1346741463712439Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGTGTAATGATCTGGACACTGTCTCAGATCGGAAGAGCACACGTCTGA201370.1333366087554815Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCAAGACATCTGATGAATAGAATCCTCTGGAAAGTGGAGATCGGAAGA200640.13285324120127034No Hit
CAAGCGGAGGAACATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCTG197670.1308866636176989Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGTGACGTGGAGACGGAGCAGTGACAGATCGGAAGAGCACACGTCTGA197190.1305688328971217Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGGAGGACCGAACCGATAAGCGTTGAAAAGCTTCCGGATGAACTGAG194510.12879427804056565No Hit
CTCGCAAGACATCTGATGAATAGAATCCTCTGGAAAGTGGAGATCGGAAG193990.12844996142660703No Hit
AATGGACGGGAGTCTGAACCAGCCAAGTAGCGTGAAGGATGACTGCCCTA192050.1271653955976075No Hit
ATGATCGGTAGGGGAGCATTCCACTCTGCGTCGAAGGTGAAGCGTGAGCT189740.12563583525482971No Hit
GAGAGTCGAACCTGATAGTATGGAGCAGATCGGAAGAGCACACGTCTAAA185110.12257009309592878Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCTGATGAATAGAATCCTCTGGAAAGTGGAGATCGGAAGAGCACACGTCT184850.12239793478894948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCCGTT179480.11884220360249204No Hit
GTGTAATGATCTGGACACTGTCTCAGATCGGAAGAGCACACGTCTGAACT178920.11847140109515196Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGATGAGTAGAATCCTCTGGAAAGTTGAGATCGGAAGAGCACACGTCTG178050.11789533291410578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACTCTAGAGAGACTGCCGTCGTAAGATGTGAGGAAGGTGGGGATGAC176420.11681603275881236No Hit
GGGCGCAGAGAATAGGTCCAGGCAACTGTTTAACAAAAACACAGGGCTGT176400.11680278981212167No Hit
GAGAGTCGAACCTGATAGTATGGAACAGATCGGAAGAGCACACGTCTGAA174170.11532620125610674Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAACATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCTGAACTCCA173790.11507458526898312Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACGTGGAGACGGAGCAGTGACAGCGCCGCGGACTGACGGAATAGTCCG169550.11226708057055117No Hit
ATAAGGGCGCTTGACTGGGAGACTAACAAGTCGATCAGGTAGGAAACTAG164600.10898945126459879No Hit
TCTGAAGGAATGTCATCCATCTTTGATGGAAGCTAACGCAGGGAACTGAA162730.10775123574901675No Hit
CCACGTGTGGAATTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC159380.1055330421783217Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTGACGTGGAGACGGAGCAGTGACAGATCGGAAGAGCACACGTCTGAAC158650.10504967462411055Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACGTAGTCGGGTTGAATATACTAAGGCGCTTGAGTGATTCACGGTTAA157330.10417564014252324No Hit
ACTAAGTTGAACTAACGAAGTCAGATTGCTAAGACAGCTAGGATGTTGGC157300.10415577572248716No Hit
AGAGTCGAACCTGATAGTATGGAAGATCGGAAGAGCACACGTCTGAACTC156800.10382470205521925Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGAACCTTACCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC153220.10145421459758096Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGTAATGATCTGGACACTGTCTCAACCGTGAGCTCAGTGAAATTAGATC153040.10133502807736451No Hit
GGGCGCAGAGAATAGGTCCAGGCAACTGTTTAACAAAAACAGATCGGAAG151370.10022924202868966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)